Novel insight into stem cell trafficking in dystrophic muscles

Andrea Farini,1,* Chiara Villa,1,* Adrian Manescu,2 Fabrizio Fiori,2 Alessandra Giuliani,2 Paola Razini,1 Clementina Sitzia,1 Giulia Del Fraro,1 Marzia Belicchi,1 Mirella Meregalli,1 Franco Rustichelli,2 Yvan Torrente1 1Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS C&am...

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Autores principales:	Farini A, Villa C, Manescu A, Fiori F, Giuliani A, Razini P, Sitzia C, Del Fraro G, Belicchi M, Meregalli M, Rustichelli F, Torrente Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d66290fc749640ee8477222e138b6f47
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spelling	oai:doaj.org-article:d66290fc749640ee8477222e138b6f472021-12-02T08:07:37ZNovel insight into stem cell trafficking in dystrophic muscles1176-91141178-2013https://doaj.org/article/d66290fc749640ee8477222e138b6f472012-06-01T00:00:00Zhttp://www.dovepress.com/novel-insight-into-stem-cell-trafficking-in-dystrophic-muscles-a10170https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Andrea Farini,1,* Chiara Villa,1,* Adrian Manescu,2 Fabrizio Fiori,2 Alessandra Giuliani,2 Paola Razini,1 Clementina Sitzia,1 Giulia Del Fraro,1 Marzia Belicchi,1 Mirella Meregalli,1 Franco Rustichelli,2 Yvan Torrente1 1Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS C&agrave; Granda Ospedale Maggiore Policlinico di Milano, Centro Dino Ferrari, Universit&agrave; di Milano, Milano, Italy; 2Universit&agrave; Politecnica delle Marche, Di.S.C.O. - Sezione di Biologia, Biochimica e Fisica Ancona, Italy*These authors contributed equally to this paperAbstract: Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications.Keywords: iron nanoparticles, micro-CT, CD133+ stem cells, dystrophic musclesFarini AVilla CManescu AFiori FGiuliani ARazini PSitzia CDel Fraro GBelicchi MMeregalli MRustichelli FTorrente YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3059-3067 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Farini A Villa C Manescu A Fiori F Giuliani A Razini P Sitzia C Del Fraro G Belicchi M Meregalli M Rustichelli F Torrente Y Novel insight into stem cell trafficking in dystrophic muscles
description	Andrea Farini,1,* Chiara Villa,1,* Adrian Manescu,2 Fabrizio Fiori,2 Alessandra Giuliani,2 Paola Razini,1 Clementina Sitzia,1 Giulia Del Fraro,1 Marzia Belicchi,1 Mirella Meregalli,1 Franco Rustichelli,2 Yvan Torrente1 1Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS C&agrave; Granda Ospedale Maggiore Policlinico di Milano, Centro Dino Ferrari, Universit&agrave; di Milano, Milano, Italy; 2Universit&agrave; Politecnica delle Marche, Di.S.C.O. - Sezione di Biologia, Biochimica e Fisica Ancona, Italy*These authors contributed equally to this paperAbstract: Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications.Keywords: iron nanoparticles, micro-CT, CD133+ stem cells, dystrophic muscles
format	article
author	Farini A Villa C Manescu A Fiori F Giuliani A Razini P Sitzia C Del Fraro G Belicchi M Meregalli M Rustichelli F Torrente Y
author_facet	Farini A Villa C Manescu A Fiori F Giuliani A Razini P Sitzia C Del Fraro G Belicchi M Meregalli M Rustichelli F Torrente Y
author_sort	Farini A
title	Novel insight into stem cell trafficking in dystrophic muscles
title_short	Novel insight into stem cell trafficking in dystrophic muscles
title_full	Novel insight into stem cell trafficking in dystrophic muscles
title_fullStr	Novel insight into stem cell trafficking in dystrophic muscles
title_full_unstemmed	Novel insight into stem cell trafficking in dystrophic muscles
title_sort	novel insight into stem cell trafficking in dystrophic muscles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/d66290fc749640ee8477222e138b6f47
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Novel insight into stem cell trafficking in dystrophic muscles

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