Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients

Obstructive sleep apnea (OSA) is a disease with great cardiovascular risk. Interleukin-8 (IL-8), an important chemokine for monocyte chemotactic migration, was studied under intermittent hypoxia condition and in OSA patients. Monocytic THP-1 cells were used to investigate the effect of intermittent...

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Autores principales:	Li-Pang Chuang, Huang-Pin Wu, Li-Ang Lee, Li-Chung Chiu, Shih-Wei Lin, Han-Chung Hu, Kuo-Chin Kao, Ning-Hung Chen, Jung-Wei Tsai, Jong-Hwei Su Pang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chemotaxis interleukin 8 intermittent hypoxia monocyte obstructive sleep apnea Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d66761e806e140f19eca4828509a7bba
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spelling	oai:doaj.org-article:d66761e806e140f19eca4828509a7bba2021-11-11T16:52:15ZElevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients10.3390/ijms2221113961422-00671661-6596https://doaj.org/article/d66761e806e140f19eca4828509a7bba2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11396https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Obstructive sleep apnea (OSA) is a disease with great cardiovascular risk. Interleukin-8 (IL-8), an important chemokine for monocyte chemotactic migration, was studied under intermittent hypoxia condition and in OSA patients. Monocytic THP-1 cells were used to investigate the effect of intermittent hypoxia on the regulation of IL-8 by an intermittent hypoxic culture system. The secreted protein and mRNA levels were studied by means of enzyme-linked immunosorbent assay and RT/real-time PCR. The chemotactic migration of monocytes toward a conditioned medium containing IL-8 was performed by means of the transwell filter migration assay. Peripheral venous blood was collected from 31 adult OSA patients and RNA was extracted from the monocytes for the analysis of IL-8 expression. The result revealed that intermittent hypoxia enhanced the monocytic THP-1 cells to actively express IL-8 at both the secreted protein and mRNA levels, which subsequently increased the migration ability of monocytes toward IL-8. The ERK, PI3K and PKC pathways were demonstrated to contribute to the activation of IL-8 expression by intermittent hypoxia. In addition, increased monocytic IL-8 expression was found in OSA patients, with disease severity dependence and diurnal changes. This study concluded the monocytic IL-8 gene expression can be activated by intermittent hypoxia and increased in OSA patients.Li-Pang ChuangHuang-Pin WuLi-Ang LeeLi-Chung ChiuShih-Wei LinHan-Chung HuKuo-Chin KaoNing-Hung ChenJung-Wei TsaiJong-Hwei Su PangMDPI AGarticlechemotaxisinterleukin 8intermittent hypoxiamonocyteobstructive sleep apneaBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11396, p 11396 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemotaxis interleukin 8 intermittent hypoxia monocyte obstructive sleep apnea Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	chemotaxis interleukin 8 intermittent hypoxia monocyte obstructive sleep apnea Biology (General) QH301-705.5 Chemistry QD1-999 Li-Pang Chuang Huang-Pin Wu Li-Ang Lee Li-Chung Chiu Shih-Wei Lin Han-Chung Hu Kuo-Chin Kao Ning-Hung Chen Jung-Wei Tsai Jong-Hwei Su Pang Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
description	Obstructive sleep apnea (OSA) is a disease with great cardiovascular risk. Interleukin-8 (IL-8), an important chemokine for monocyte chemotactic migration, was studied under intermittent hypoxia condition and in OSA patients. Monocytic THP-1 cells were used to investigate the effect of intermittent hypoxia on the regulation of IL-8 by an intermittent hypoxic culture system. The secreted protein and mRNA levels were studied by means of enzyme-linked immunosorbent assay and RT/real-time PCR. The chemotactic migration of monocytes toward a conditioned medium containing IL-8 was performed by means of the transwell filter migration assay. Peripheral venous blood was collected from 31 adult OSA patients and RNA was extracted from the monocytes for the analysis of IL-8 expression. The result revealed that intermittent hypoxia enhanced the monocytic THP-1 cells to actively express IL-8 at both the secreted protein and mRNA levels, which subsequently increased the migration ability of monocytes toward IL-8. The ERK, PI3K and PKC pathways were demonstrated to contribute to the activation of IL-8 expression by intermittent hypoxia. In addition, increased monocytic IL-8 expression was found in OSA patients, with disease severity dependence and diurnal changes. This study concluded the monocytic IL-8 gene expression can be activated by intermittent hypoxia and increased in OSA patients.
format	article
author	Li-Pang Chuang Huang-Pin Wu Li-Ang Lee Li-Chung Chiu Shih-Wei Lin Han-Chung Hu Kuo-Chin Kao Ning-Hung Chen Jung-Wei Tsai Jong-Hwei Su Pang
author_facet	Li-Pang Chuang Huang-Pin Wu Li-Ang Lee Li-Chung Chiu Shih-Wei Lin Han-Chung Hu Kuo-Chin Kao Ning-Hung Chen Jung-Wei Tsai Jong-Hwei Su Pang
author_sort	Li-Pang Chuang
title	Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
title_short	Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
title_full	Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
title_fullStr	Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
title_full_unstemmed	Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients
title_sort	elevated monocytic interleukin-8 expression under intermittent hypoxia condition and in obstructive sleep apnea patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d66761e806e140f19eca4828509a7bba
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Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients

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