Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease

Abstract. Wiraswati SM, Nawangsih AA, Rusmana I, Wahyudi AT. 2020. Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease. Biodiversitas 21: 1273-1278. In vitro analysis of rice phyllosphere bacteria can be developed as biocontrol agents of blast dise...

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Autores principales:	SRI MARTINA WIRASWATI, ABDJAD ASIH NAWANGSIH, IMAN RUSMANA, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacillus, bioactive compounds, bioautography, pyricularia oryzae, tlc Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d6686377dc364e27a3a3b38d518f8385
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spelling	oai:doaj.org-article:d6686377dc364e27a3a3b38d518f83852021-11-22T00:34:40ZRice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease1412-033X2085-472210.13057/biodiv/d210401https://doaj.org/article/d6686377dc364e27a3a3b38d518f83852020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5204https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wiraswati SM, Nawangsih AA, Rusmana I, Wahyudi AT. 2020. Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease. Biodiversitas 21: 1273-1278. In vitro analysis of rice phyllosphere bacteria can be developed as biocontrol agents of blast disease using their antifungal activity. To prove the efficacy of bacterial isolates in reducing the severity of blast disease, a greenhouse experiment was conducted. Furthermore, antifungal compounds produced by potential bacterial isolate were also identified using Thin Layer Chromatography (TLC) and Liquid Chromatography-Mass Spectrometry/ Mass Spectrometry (LC-MS/MS) analysis. The application of seven rice phyllosphere bacterial isolates significantly reduced the severity of blast disease in rice (var: Ciherang). Surprisingly, the isolate STGG 14 is the most effective with 70.83% of blast disease reduction. Isolate STGG 14 has been identified as Bacillus subtilis subsp. subtilis which is known as various bioactive compounds producer. It was confirmed through identification of antifungal compounds produced by isolate STGG 14. The bioautography test shows that crude bioactive compounds from isolate STGG 14 could inhibit P. oryzae race 173. On the further analysis using LC-MS/MS, four bioactive compounds isolated from isolate STGG 14 were identified as moracin C, psoralen, 4,6-dimethyl-3 (4’-hydroxyphenyl) coumarin and xanthotoxin. All identified compounds except moracin C, belong to the coumarin group. According to the previous studies, among all compounds, psoralen and xanthotoxin displayed antifungal activity against several fungal pathogens.SRI MARTINA WIRASWATIABDJAD ASIH NAWANGSIHIMAN RUSMANAAris Tri WahyudiMBI & UNS Soloarticlebacillus, bioactive compounds, bioautography, pyricularia oryzae, tlcBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus, bioactive compounds, bioautography, pyricularia oryzae, tlc Biology (General) QH301-705.5
spellingShingle	bacillus, bioactive compounds, bioautography, pyricularia oryzae, tlc Biology (General) QH301-705.5 SRI MARTINA WIRASWATI ABDJAD ASIH NAWANGSIH IMAN RUSMANA Aris Tri Wahyudi Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
description	Abstract. Wiraswati SM, Nawangsih AA, Rusmana I, Wahyudi AT. 2020. Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease. Biodiversitas 21: 1273-1278. In vitro analysis of rice phyllosphere bacteria can be developed as biocontrol agents of blast disease using their antifungal activity. To prove the efficacy of bacterial isolates in reducing the severity of blast disease, a greenhouse experiment was conducted. Furthermore, antifungal compounds produced by potential bacterial isolate were also identified using Thin Layer Chromatography (TLC) and Liquid Chromatography-Mass Spectrometry/ Mass Spectrometry (LC-MS/MS) analysis. The application of seven rice phyllosphere bacterial isolates significantly reduced the severity of blast disease in rice (var: Ciherang). Surprisingly, the isolate STGG 14 is the most effective with 70.83% of blast disease reduction. Isolate STGG 14 has been identified as Bacillus subtilis subsp. subtilis which is known as various bioactive compounds producer. It was confirmed through identification of antifungal compounds produced by isolate STGG 14. The bioautography test shows that crude bioactive compounds from isolate STGG 14 could inhibit P. oryzae race 173. On the further analysis using LC-MS/MS, four bioactive compounds isolated from isolate STGG 14 were identified as moracin C, psoralen, 4,6-dimethyl-3 (4’-hydroxyphenyl) coumarin and xanthotoxin. All identified compounds except moracin C, belong to the coumarin group. According to the previous studies, among all compounds, psoralen and xanthotoxin displayed antifungal activity against several fungal pathogens.
format	article
author	SRI MARTINA WIRASWATI ABDJAD ASIH NAWANGSIH IMAN RUSMANA Aris Tri Wahyudi
author_facet	SRI MARTINA WIRASWATI ABDJAD ASIH NAWANGSIH IMAN RUSMANA Aris Tri Wahyudi
author_sort	SRI MARTINA WIRASWATI
title	Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
title_short	Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
title_full	Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
title_fullStr	Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
title_full_unstemmed	Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
title_sort	rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d6686377dc364e27a3a3b38d518f8385
work_keys_str_mv	AT srimartinawiraswati ricephyllospherebacteriaproducingantifungalcompoundsasbiologicalcontrolagentsofblastdisease AT abdjadasihnawangsih ricephyllospherebacteriaproducingantifungalcompoundsasbiologicalcontrolagentsofblastdisease AT imanrusmana ricephyllospherebacteriaproducingantifungalcompoundsasbiologicalcontrolagentsofblastdisease AT aristriwahyudi ricephyllospherebacteriaproducingantifungalcompoundsasbiologicalcontrolagentsofblastdisease
_version_	1718418611936165888

Rice phyllosphere bacteria producing antifungal compounds as biological control agents of blast disease

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