Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage

Abstract Gut microbiota disorder will lead to intestinal damage. This study evaluated the influence of total diterpenoids extracted from Euphorbia pekinensis (TDEP) on gut microbiota and intestinal mucosal barrier after long‐term administration, and the correlations between gut microbiota and intest...

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Autores principales:	Kuilong Wang, Xiaofen Xu, Aikebaier Maimaiti, Min Hao, Xianan Sang, Qiyuan Shan, Xin Wu, Gang Cao
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	diterpenoids Euphorbia pekinensis gut microbiota intestinal mucosal intestinal toxicity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d66c575c43124eac84f5f0102ea1e28a
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spelling	oai:doaj.org-article:d66c575c43124eac84f5f0102ea1e28a2021-11-16T13:45:54ZGut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage2052-170710.1002/prp2.765https://doaj.org/article/d66c575c43124eac84f5f0102ea1e28a2021-10-01T00:00:00Zhttps://doi.org/10.1002/prp2.765https://doaj.org/toc/2052-1707Abstract Gut microbiota disorder will lead to intestinal damage. This study evaluated the influence of total diterpenoids extracted from Euphorbia pekinensis (TDEP) on gut microbiota and intestinal mucosal barrier after long‐term administration, and the correlations between gut microbiota and intestinal mucosal barrier were analysed by Spearman correlation analysis. Mice were randomly divided to control group, TDEP groups (4, 8, 16 mg/kg), TDEP (16 mg/kg) + antibiotic group. Two weeks after intragastric administration, inflammatory factors (TNF‐α, IL‐6, IL‐1β) and LPS in serum, short chain fatty acids (SCFAs) in feces were tested by Enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography (HPLC), respectively. The expression of tight junction (TJ) protein in colon was measured by western blotting. Furthermore, the effects of TDEP on gut microbiota community in mice have been investigated by 16SrDNA high‐throughput sequencing. The results showed TDEP significantly increased the levels of inflammatory factors in dose‐dependent manners, and decreased the expression of TJ protein and SCFAs, and the composition of gut microbiota of mice in TDEP group was significantly different from that of control group. When antibiotics were added, the diversity of gut microbiota was significantly reduced, and the colon injury was more serious. Finally, through correlation analysis, we have found nine key bacteria (Barnesiella, Muribaculaceae_unclassified, Alloprevotella, Candidatus_Arthromitus, Enterorhabdus, Alistipes, Bilophila, Mucispirillum, Ruminiclostridium) that may be related to colon injury caused by TDEP. Taken together, the disturbance of gut microbiota caused by TDEP may aggravate the colon injury, and its possible mechanism may be related to the decrease of SCFAs in feces, disrupted the expression of TJ protein in colon and increasing the contents of inflammatory factors.Kuilong WangXiaofen XuAikebaier MaimaitiMin HaoXianan SangQiyuan ShanXin WuGang CaoWileyarticlediterpenoidsEuphorbia pekinensisgut microbiotaintestinal mucosalintestinal toxicityTherapeutics. PharmacologyRM1-950ENPharmacology Research & Perspectives, Vol 9, Iss 5, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diterpenoids Euphorbia pekinensis gut microbiota intestinal mucosal intestinal toxicity Therapeutics. Pharmacology RM1-950
spellingShingle	diterpenoids Euphorbia pekinensis gut microbiota intestinal mucosal intestinal toxicity Therapeutics. Pharmacology RM1-950 Kuilong Wang Xiaofen Xu Aikebaier Maimaiti Min Hao Xianan Sang Qiyuan Shan Xin Wu Gang Cao Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
description	Abstract Gut microbiota disorder will lead to intestinal damage. This study evaluated the influence of total diterpenoids extracted from Euphorbia pekinensis (TDEP) on gut microbiota and intestinal mucosal barrier after long‐term administration, and the correlations between gut microbiota and intestinal mucosal barrier were analysed by Spearman correlation analysis. Mice were randomly divided to control group, TDEP groups (4, 8, 16 mg/kg), TDEP (16 mg/kg) + antibiotic group. Two weeks after intragastric administration, inflammatory factors (TNF‐α, IL‐6, IL‐1β) and LPS in serum, short chain fatty acids (SCFAs) in feces were tested by Enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography (HPLC), respectively. The expression of tight junction (TJ) protein in colon was measured by western blotting. Furthermore, the effects of TDEP on gut microbiota community in mice have been investigated by 16SrDNA high‐throughput sequencing. The results showed TDEP significantly increased the levels of inflammatory factors in dose‐dependent manners, and decreased the expression of TJ protein and SCFAs, and the composition of gut microbiota of mice in TDEP group was significantly different from that of control group. When antibiotics were added, the diversity of gut microbiota was significantly reduced, and the colon injury was more serious. Finally, through correlation analysis, we have found nine key bacteria (Barnesiella, Muribaculaceae_unclassified, Alloprevotella, Candidatus_Arthromitus, Enterorhabdus, Alistipes, Bilophila, Mucispirillum, Ruminiclostridium) that may be related to colon injury caused by TDEP. Taken together, the disturbance of gut microbiota caused by TDEP may aggravate the colon injury, and its possible mechanism may be related to the decrease of SCFAs in feces, disrupted the expression of TJ protein in colon and increasing the contents of inflammatory factors.
format	article
author	Kuilong Wang Xiaofen Xu Aikebaier Maimaiti Min Hao Xianan Sang Qiyuan Shan Xin Wu Gang Cao
author_facet	Kuilong Wang Xiaofen Xu Aikebaier Maimaiti Min Hao Xianan Sang Qiyuan Shan Xin Wu Gang Cao
author_sort	Kuilong Wang
title	Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
title_short	Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
title_full	Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
title_fullStr	Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
title_full_unstemmed	Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage
title_sort	gut microbiota disorder caused by diterpenoids extracted from euphorbia pekinensis aggravates intestinal mucosal damage
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/d66c575c43124eac84f5f0102ea1e28a
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Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage

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