A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells

Jianmeng Zhu,1,* Yiping Li,1,* Lei Li,2 Jian Wang,1 Hongqin Wang,1 Wenzhong Hong,1 Ke Hao,3 Yadan Xue,3 Bingyu Chen,1,3 Zhen Wang1,3 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Ha...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhu J, Li Y, Li L, Wang J, Wang H, Hong W, Hao K, Xue Y, Chen B, Wang Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	tetramer graphene hemin major histocompatibility complex multimer cytotoxic T lymphocytes hepatocellular carcinoma. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d67114c9163f46ff91f7bf2511dfc6b9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d67114c9163f46ff91f7bf2511dfc6b9
record_format	dspace
spelling	oai:doaj.org-article:d67114c9163f46ff91f7bf2511dfc6b92021-12-02T07:25:22ZA novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells1178-2013https://doaj.org/article/d67114c9163f46ff91f7bf2511dfc6b92018-09-01T00:00:00Zhttps://www.dovepress.com/a-novel-absorption-spectrometric-method-based-on-graphene-nanomaterial-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jianmeng Zhu,1,* Yiping Li,1,* Lei Li,2 Jian Wang,1 Hongqin Wang,1 Wenzhong Hong,1 Ke Hao,3 Yadan Xue,3 Bingyu Chen,1,3 Zhen Wang1,3 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang Province, China; 2Department of Pathophysiology, School of Basic Medical Science, Southern Medical University, Guangzhou, Zhejiang, China; 3Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China *These authors contributed equally to this work Introduction: Detection of antigen-specific cytotoxic T lymphocytes (CTLs) is the foundation for understanding hepatocellular carcinoma immune pathology and hepatocellular carcinoma immunotherapy. However, the classical method for labeling CTLs, major histocompatibility complex (MHC)–peptide tetramer, has drawbacks and needs further improvement. Materials and methods: Here, as a new detection probe, a graphene-based MHC–peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO–streptavidin complex. Biotinylated MHC–peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine. Results: Using HRGO/MHC–peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients. Conclusion: HRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells. Keywords: tetramer, graphene, hemin, major histocompatibility complex multimer, cytotoxic T lymphocytes, hepatocellular carcinomaZhu JLi YLi LWang JWang HHong WHao KXue YChen BWang ZDove Medical Pressarticletetramergrapheneheminmajor histocompatibility complex multimercytotoxic T lymphocyteshepatocellular carcinoma.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5523-5536 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	tetramer graphene hemin major histocompatibility complex multimer cytotoxic T lymphocytes hepatocellular carcinoma. Medicine (General) R5-920
spellingShingle	tetramer graphene hemin major histocompatibility complex multimer cytotoxic T lymphocytes hepatocellular carcinoma. Medicine (General) R5-920 Zhu J Li Y Li L Wang J Wang H Hong W Hao K Xue Y Chen B Wang Z A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
description	Jianmeng Zhu,1,* Yiping Li,1,* Lei Li,2 Jian Wang,1 Hongqin Wang,1 Wenzhong Hong,1 Ke Hao,3 Yadan Xue,3 Bingyu Chen,1,3 Zhen Wang1,3 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang Province, China; 2Department of Pathophysiology, School of Basic Medical Science, Southern Medical University, Guangzhou, Zhejiang, China; 3Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China *These authors contributed equally to this work Introduction: Detection of antigen-specific cytotoxic T lymphocytes (CTLs) is the foundation for understanding hepatocellular carcinoma immune pathology and hepatocellular carcinoma immunotherapy. However, the classical method for labeling CTLs, major histocompatibility complex (MHC)–peptide tetramer, has drawbacks and needs further improvement. Materials and methods: Here, as a new detection probe, a graphene-based MHC–peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO–streptavidin complex. Biotinylated MHC–peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine. Results: Using HRGO/MHC–peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients. Conclusion: HRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells. Keywords: tetramer, graphene, hemin, major histocompatibility complex multimer, cytotoxic T lymphocytes, hepatocellular carcinoma
format	article
author	Zhu J Li Y Li L Wang J Wang H Hong W Hao K Xue Y Chen B Wang Z
author_facet	Zhu J Li Y Li L Wang J Wang H Hong W Hao K Xue Y Chen B Wang Z
author_sort	Zhu J
title	A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
title_short	A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
title_full	A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
title_fullStr	A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
title_full_unstemmed	A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells
title_sort	novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific t lymphocyte cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/d67114c9163f46ff91f7bf2511dfc6b9
work_keys_str_mv	AT zhuj anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT liy anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT lil anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangj anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangh anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT hongw anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT haok anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT xuey anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT chenb anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangz anovelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT zhuj novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT liy novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT lil novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangj novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangh novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT hongw novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT haok novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT xuey novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT chenb novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells AT wangz novelabsorptionspectrometricmethodbasedongraphenenanomaterialsfordetectionofhepatocellularcarcinomaspecifictlymphocytecells
_version_	1718399427809378304

A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells

Ejemplares similares