Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm

Neural stem cells (NSCs) are multipotent cells capable of self-renewal and differentiation into different nervous system cells. Mouse NSCs (mNSCs) are useful tools for studying neurogenesis and the therapeutic applications of neurodegenerative diseases in mammals. Formyl peptide receptor 2 (FPR2), e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang Woo Kwon, Sungwon Bae, Yeon Suk Jo, Youngsuk Seo, Jong Hyuk Yoon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	neural stem cells formyl peptide receptor 2 migration expansion WKYMVm Science Q
Acceso en línea:	https://doaj.org/article/d67c843cb90b4676be97a8c2ceb12c04
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d67c843cb90b4676be97a8c2ceb12c04
record_format	dspace
spelling	oai:doaj.org-article:d67c843cb90b4676be97a8c2ceb12c042021-11-25T18:11:34ZStimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm10.3390/life111112482075-1729https://doaj.org/article/d67c843cb90b4676be97a8c2ceb12c042021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1248https://doaj.org/toc/2075-1729Neural stem cells (NSCs) are multipotent cells capable of self-renewal and differentiation into different nervous system cells. Mouse NSCs (mNSCs) are useful tools for studying neurogenesis and the therapeutic applications of neurodegenerative diseases in mammals. Formyl peptide receptor 2 (FPR2), expressed in the central nervous system and brain, is involved in the migration and differentiation of murine embryonic-derived NSCs. In this study, we explored the effect of FPR2 activation in adult mNSCs using the synthetic peptide Trp-Lys-Tyr-Met-Val-D-Met-NH2 (WKYMVm), an agonist of FPR2. After isolation of NSCs from the subventricular zone of the adult mouse brain, they were cultured in two culture systems—neurospheres or adherent monolayers—to demonstrate the expression of NSC markers and phenotypes. Under different conditions, mNSCs differentiated into neurons and glial cells such as astrocytes, microglia, and oligodendrocytes. Treatment with WKYMVm stimulated the chemotactic migration of mNSCs. Moreover, WKYMVm-treated mNSCs were found to promote proliferation; this result was confirmed by the expansion of mNSCs in Matrigel and the increase in the number of Ki67-positive cells. Incubation of mNSCs with WKYMVm in a supplement-free medium enhanced the survival rate of the mNSCs. Together, these results suggest that WKYMVm-induced activation of FPR2 stimulates cellular responses in adult NSCs.Yang Woo KwonSungwon BaeYeon Suk JoYoungsuk SeoJong Hyuk YoonMDPI AGarticleneural stem cellsformyl peptide receptor 2migrationexpansionWKYMVmScienceQENLife, Vol 11, Iss 1248, p 1248 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neural stem cells formyl peptide receptor 2 migration expansion WKYMVm Science Q
spellingShingle	neural stem cells formyl peptide receptor 2 migration expansion WKYMVm Science Q Yang Woo Kwon Sungwon Bae Yeon Suk Jo Youngsuk Seo Jong Hyuk Yoon Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
description	Neural stem cells (NSCs) are multipotent cells capable of self-renewal and differentiation into different nervous system cells. Mouse NSCs (mNSCs) are useful tools for studying neurogenesis and the therapeutic applications of neurodegenerative diseases in mammals. Formyl peptide receptor 2 (FPR2), expressed in the central nervous system and brain, is involved in the migration and differentiation of murine embryonic-derived NSCs. In this study, we explored the effect of FPR2 activation in adult mNSCs using the synthetic peptide Trp-Lys-Tyr-Met-Val-D-Met-NH2 (WKYMVm), an agonist of FPR2. After isolation of NSCs from the subventricular zone of the adult mouse brain, they were cultured in two culture systems—neurospheres or adherent monolayers—to demonstrate the expression of NSC markers and phenotypes. Under different conditions, mNSCs differentiated into neurons and glial cells such as astrocytes, microglia, and oligodendrocytes. Treatment with WKYMVm stimulated the chemotactic migration of mNSCs. Moreover, WKYMVm-treated mNSCs were found to promote proliferation; this result was confirmed by the expansion of mNSCs in Matrigel and the increase in the number of Ki67-positive cells. Incubation of mNSCs with WKYMVm in a supplement-free medium enhanced the survival rate of the mNSCs. Together, these results suggest that WKYMVm-induced activation of FPR2 stimulates cellular responses in adult NSCs.
format	article
author	Yang Woo Kwon Sungwon Bae Yeon Suk Jo Youngsuk Seo Jong Hyuk Yoon
author_facet	Yang Woo Kwon Sungwon Bae Yeon Suk Jo Youngsuk Seo Jong Hyuk Yoon
author_sort	Yang Woo Kwon
title	Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
title_short	Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
title_full	Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
title_fullStr	Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
title_full_unstemmed	Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
title_sort	stimulation of the migration and expansion of adult mouse neural stem cells by the fpr2-specific peptide wkymvm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d67c843cb90b4676be97a8c2ceb12c04
work_keys_str_mv	AT yangwookwon stimulationofthemigrationandexpansionofadultmouseneuralstemcellsbythefpr2specificpeptidewkymvm AT sungwonbae stimulationofthemigrationandexpansionofadultmouseneuralstemcellsbythefpr2specificpeptidewkymvm AT yeonsukjo stimulationofthemigrationandexpansionofadultmouseneuralstemcellsbythefpr2specificpeptidewkymvm AT youngsukseo stimulationofthemigrationandexpansionofadultmouseneuralstemcellsbythefpr2specificpeptidewkymvm AT jonghyukyoon stimulationofthemigrationandexpansionofadultmouseneuralstemcellsbythefpr2specificpeptidewkymvm
_version_	1718411527426408448

Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm

Ejemplares similares