DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS

Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the st...

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Autores principales:	E. V. Grigorova, L. V. Rychkova, E. I. Ivanova, U. M. Nemchenko, M. V. Savelkaeva
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	klebsiella spp. Science Q
Acceso en línea:	https://doaj.org/article/d67e015c998543b3804b81c59c2c99c0
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spelling	oai:doaj.org-article:d67e015c998543b3804b81c59c2c99c02021-11-23T06:14:41ZDETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS2541-94202587-959610.29413/ABS.2018-3.5.9https://doaj.org/article/d67e015c998543b3804b81c59c2c99c02018-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/708https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological study composition of the intestinal contents was carried out according to the Industry standard “Protocol of management of patients. Intestinal dysbiosis” (2003). Identification was carried out according to generally accepted schemes using commercial test systems for biochemical identification of bacteria. Statistical data processing was performed using licensed applications “MS Office Excel 2003 for Windows 7”. The data on the quantitative and qualitative changes in the composition microbiota in the comparison groups were obtained. The results of detection genetic determinants of pathogenicity in the samples of Klebsiella of two species show that among the strains of Klebsiella spp., vegetating in the intestines of children as a component of an allochthonous microbiota, a sufficiently high and virulent potential can be concentrated. Detection of pathogenicity genes in bacteria of the genus Klebsiella will expand and deepen the problem of finding the structures of adaptation of strains of bacteria that cause FGID in children of the first year of life.E. V. GrigorovaL. V. RychkovaE. I. IvanovaU. M. NemchenkoM. V. SavelkaevaScientific Сentre for Family Health and Human Reproduction Problemsarticleklebsiella spp.ScienceQRUActa Biomedica Scientifica, Vol 3, Iss 5, Pp 60-65 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	klebsiella spp. Science Q
spellingShingle	klebsiella spp. Science Q E. V. Grigorova L. V. Rychkova E. I. Ivanova U. M. Nemchenko M. V. Savelkaeva DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
description	Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological study composition of the intestinal contents was carried out according to the Industry standard “Protocol of management of patients. Intestinal dysbiosis” (2003). Identification was carried out according to generally accepted schemes using commercial test systems for biochemical identification of bacteria. Statistical data processing was performed using licensed applications “MS Office Excel 2003 for Windows 7”. The data on the quantitative and qualitative changes in the composition microbiota in the comparison groups were obtained. The results of detection genetic determinants of pathogenicity in the samples of Klebsiella of two species show that among the strains of Klebsiella spp., vegetating in the intestines of children as a component of an allochthonous microbiota, a sufficiently high and virulent potential can be concentrated. Detection of pathogenicity genes in bacteria of the genus Klebsiella will expand and deepen the problem of finding the structures of adaptation of strains of bacteria that cause FGID in children of the first year of life.
format	article
author	E. V. Grigorova L. V. Rychkova E. I. Ivanova U. M. Nemchenko M. V. Savelkaeva
author_facet	E. V. Grigorova L. V. Rychkova E. I. Ivanova U. M. Nemchenko M. V. Savelkaeva
author_sort	E. V. Grigorova
title	DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
title_short	DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
title_full	DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
title_fullStr	DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
title_full_unstemmed	DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS
title_sort	detection of genetic determinants of pathogenicity of strains of klebsiella spp. isolated from the intestinal biotope of children with functional gastrointestinal disorders
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/d67e015c998543b3804b81c59c2c99c0
work_keys_str_mv	AT evgrigorova detectionofgeneticdeterminantsofpathogenicityofstrainsofklebsiellasppisolatedfromtheintestinalbiotopeofchildrenwithfunctionalgastrointestinaldisorders AT lvrychkova detectionofgeneticdeterminantsofpathogenicityofstrainsofklebsiellasppisolatedfromtheintestinalbiotopeofchildrenwithfunctionalgastrointestinaldisorders AT eiivanova detectionofgeneticdeterminantsofpathogenicityofstrainsofklebsiellasppisolatedfromtheintestinalbiotopeofchildrenwithfunctionalgastrointestinaldisorders AT umnemchenko detectionofgeneticdeterminantsofpathogenicityofstrainsofklebsiellasppisolatedfromtheintestinalbiotopeofchildrenwithfunctionalgastrointestinaldisorders AT mvsavelkaeva detectionofgeneticdeterminantsofpathogenicityofstrainsofklebsiellasppisolatedfromtheintestinalbiotopeofchildrenwithfunctionalgastrointestinaldisorders
_version_	1718416977273290752

DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS

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