Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival
Random flaps are widely used in tissue reconstruction, attributed to the lack of vascular axial limitation. Nevertheless, the distal end of the flap is prone to necrosis due to the lack of blood supply. Notoginseng triterpenes (NTs) are the active components extracted from Panax notoginseng, reducin...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:d68307aa29174d1286f158abce3cc61c2021-11-19T07:11:48ZNotoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival2296-418510.3389/fbioe.2021.771066https://doaj.org/article/d68307aa29174d1286f158abce3cc61c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.771066/fullhttps://doaj.org/toc/2296-4185Random flaps are widely used in tissue reconstruction, attributed to the lack of vascular axial limitation. Nevertheless, the distal end of the flap is prone to necrosis due to the lack of blood supply. Notoginseng triterpenes (NTs) are the active components extracted from Panax notoginseng, reducing oxygen consumption and improving the body’s tolerance to hypoxia. However, their role in random flap survival has not been elucidated. In this study, we used a mouse random skin flap model to verify that NT can promote cell proliferation and migration and that increasing blood perfusion can effectively improve the survival area of a skin flap. Our study also showed that the autophagy of random flaps after NT treatment was activated through the Beclin-1/VPS34/LC3 signaling pathway, and the therapeutic effect of NT significantly decreased after VPS34 IN inhibited autophagy. In conclusion, we have demonstrated that NT can significantly improve the survival rate of random flaps through the Beclin-1/VPS34/LC3 signaling pathway, suggesting that it might be a promising clinical treatment option.Zhiyang HuangXiaobin LuoYifan ZhangYibo YingXiong CaiWenjie LuJuan ZhaoYutian WangWenwei LinYurong TuZiyue XiangQiuji WuShengwu YangSipin ZhuXiaoyang LiFrontiers Media S.A.articlerandom flapnotoginseng triterpenesoxidative stressautophagyangiogenesisBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
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DOAJ |
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| topic |
random flap notoginseng triterpenes oxidative stress autophagy angiogenesis Biotechnology TP248.13-248.65 |
| spellingShingle |
random flap notoginseng triterpenes oxidative stress autophagy angiogenesis Biotechnology TP248.13-248.65 Zhiyang Huang Xiaobin Luo Yifan Zhang Yibo Ying Xiong Cai Wenjie Lu Juan Zhao Yutian Wang Wenwei Lin Yurong Tu Ziyue Xiang Qiuji Wu Shengwu Yang Sipin Zhu Xiaoyang Li Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| description |
Random flaps are widely used in tissue reconstruction, attributed to the lack of vascular axial limitation. Nevertheless, the distal end of the flap is prone to necrosis due to the lack of blood supply. Notoginseng triterpenes (NTs) are the active components extracted from Panax notoginseng, reducing oxygen consumption and improving the body’s tolerance to hypoxia. However, their role in random flap survival has not been elucidated. In this study, we used a mouse random skin flap model to verify that NT can promote cell proliferation and migration and that increasing blood perfusion can effectively improve the survival area of a skin flap. Our study also showed that the autophagy of random flaps after NT treatment was activated through the Beclin-1/VPS34/LC3 signaling pathway, and the therapeutic effect of NT significantly decreased after VPS34 IN inhibited autophagy. In conclusion, we have demonstrated that NT can significantly improve the survival rate of random flaps through the Beclin-1/VPS34/LC3 signaling pathway, suggesting that it might be a promising clinical treatment option. |
| format |
article |
| author |
Zhiyang Huang Xiaobin Luo Yifan Zhang Yibo Ying Xiong Cai Wenjie Lu Juan Zhao Yutian Wang Wenwei Lin Yurong Tu Ziyue Xiang Qiuji Wu Shengwu Yang Sipin Zhu Xiaoyang Li |
| author_facet |
Zhiyang Huang Xiaobin Luo Yifan Zhang Yibo Ying Xiong Cai Wenjie Lu Juan Zhao Yutian Wang Wenwei Lin Yurong Tu Ziyue Xiang Qiuji Wu Shengwu Yang Sipin Zhu Xiaoyang Li |
| author_sort |
Zhiyang Huang |
| title |
Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| title_short |
Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| title_full |
Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| title_fullStr |
Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| title_full_unstemmed |
Notoginseng Triterpenes Inhibited Autophagy in Random Flaps via the Beclin-1/VPS34/LC3 Signaling Pathway to Improve Tissue Survival |
| title_sort |
notoginseng triterpenes inhibited autophagy in random flaps via the beclin-1/vps34/lc3 signaling pathway to improve tissue survival |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/d68307aa29174d1286f158abce3cc61c |
| work_keys_str_mv |
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| _version_ |
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