Dried blood spot sampling for hepatitis B virus serology and molecular testing.

<h4>Background aims</h4>Dried blood spots (DBS) on filter paper have been successfully used to diagnose and monitor several infectious diseases. The aim was to investigate the performance of DBS in hepatitis B virus (HBV) diagnosis using commercial tests in comparison to standard methods...

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Autores principales:	Sofiane Mohamed, Audrey Raimondo, Guillaume Pénaranda, Claire Camus, Denis Ouzan, Sophie Ravet, Marc Bourlière, Hacène Khiri, Patrick Dukan, Daniel Olive, Philippe Halfon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d68e0929fd2d425a9b8373e8a87ce12a
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spelling	oai:doaj.org-article:d68e0929fd2d425a9b8373e8a87ce12a2021-11-18T07:49:22ZDried blood spot sampling for hepatitis B virus serology and molecular testing.1932-620310.1371/journal.pone.0061077https://doaj.org/article/d68e0929fd2d425a9b8373e8a87ce12a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613788/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background aims</h4>Dried blood spots (DBS) on filter paper have been successfully used to diagnose and monitor several infectious diseases. The aim was to investigate the performance of DBS in hepatitis B virus (HBV) diagnosis using commercial tests in comparison to standard methods.<h4>Methods</h4>Paired DBS and plasma samples were collected from 200 patients: 100 patients with HBsAg negative status and 100 patients with HBsAg positive status. In the latter patient, HBeAg reactivity was tested. Ten samples of anti-HBs were collected from people vaccinated against HBV. We also studied 50 patients with positive HBV DNA viral load in plasma and 10 HBV DNA negative patients. HBV genotypes and gene polymerase mutations were determined in 10 randomly selected HBV-infected patients. The DBS sample consisted of 50 µL of whole blood, i.e. a 12-mm paper card.<h4>Results</h4>The sensitivity thresholds of HBsAg and anti-HBs antibody were 0.30 ± 0.08 IU/mL and 18.11 ± 6.05 IU/mL, respectively, for DBS with 98% sensitivity and 100% specificity. Sensitivity was 98% and specificity 100% for the detection of HBV DNA on a blotter, considering an HBV DNA threshold of 914.1 ± 157.8 IU/ml. Ten patients had an HBeAg positive status in plasma, all were detected positive using DBS. HBV genotyping and mutation detection were successfully performed on DBS, with full concordance between the 10 paired DBS and plasma samples.<h4>Conclusion</h4>This study shows DBS is a reliable alternative to plasma specimens for quantifying and detecting HBsAg, anti-HBs, HBeAg and genotyping. DBS may increase the opportunities for HBV testing and treatment follow-up in hard-to-reach individuals.Sofiane MohamedAudrey RaimondoGuillaume PénarandaClaire CamusDenis OuzanSophie RavetMarc BourlièreHacène KhiriPatrick DukanDaniel OlivePhilippe HalfonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61077 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sofiane Mohamed Audrey Raimondo Guillaume Pénaranda Claire Camus Denis Ouzan Sophie Ravet Marc Bourlière Hacène Khiri Patrick Dukan Daniel Olive Philippe Halfon Dried blood spot sampling for hepatitis B virus serology and molecular testing.
description	<h4>Background aims</h4>Dried blood spots (DBS) on filter paper have been successfully used to diagnose and monitor several infectious diseases. The aim was to investigate the performance of DBS in hepatitis B virus (HBV) diagnosis using commercial tests in comparison to standard methods.<h4>Methods</h4>Paired DBS and plasma samples were collected from 200 patients: 100 patients with HBsAg negative status and 100 patients with HBsAg positive status. In the latter patient, HBeAg reactivity was tested. Ten samples of anti-HBs were collected from people vaccinated against HBV. We also studied 50 patients with positive HBV DNA viral load in plasma and 10 HBV DNA negative patients. HBV genotypes and gene polymerase mutations were determined in 10 randomly selected HBV-infected patients. The DBS sample consisted of 50 µL of whole blood, i.e. a 12-mm paper card.<h4>Results</h4>The sensitivity thresholds of HBsAg and anti-HBs antibody were 0.30 ± 0.08 IU/mL and 18.11 ± 6.05 IU/mL, respectively, for DBS with 98% sensitivity and 100% specificity. Sensitivity was 98% and specificity 100% for the detection of HBV DNA on a blotter, considering an HBV DNA threshold of 914.1 ± 157.8 IU/ml. Ten patients had an HBeAg positive status in plasma, all were detected positive using DBS. HBV genotyping and mutation detection were successfully performed on DBS, with full concordance between the 10 paired DBS and plasma samples.<h4>Conclusion</h4>This study shows DBS is a reliable alternative to plasma specimens for quantifying and detecting HBsAg, anti-HBs, HBeAg and genotyping. DBS may increase the opportunities for HBV testing and treatment follow-up in hard-to-reach individuals.
format	article
author	Sofiane Mohamed Audrey Raimondo Guillaume Pénaranda Claire Camus Denis Ouzan Sophie Ravet Marc Bourlière Hacène Khiri Patrick Dukan Daniel Olive Philippe Halfon
author_facet	Sofiane Mohamed Audrey Raimondo Guillaume Pénaranda Claire Camus Denis Ouzan Sophie Ravet Marc Bourlière Hacène Khiri Patrick Dukan Daniel Olive Philippe Halfon
author_sort	Sofiane Mohamed
title	Dried blood spot sampling for hepatitis B virus serology and molecular testing.
title_short	Dried blood spot sampling for hepatitis B virus serology and molecular testing.
title_full	Dried blood spot sampling for hepatitis B virus serology and molecular testing.
title_fullStr	Dried blood spot sampling for hepatitis B virus serology and molecular testing.
title_full_unstemmed	Dried blood spot sampling for hepatitis B virus serology and molecular testing.
title_sort	dried blood spot sampling for hepatitis b virus serology and molecular testing.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d68e0929fd2d425a9b8373e8a87ce12a
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Dried blood spot sampling for hepatitis B virus serology and molecular testing.

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