Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases

Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were t...

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Autores principales:	EL Hassouni Youssef, Bourhia Mohammed, Bari Ahmed, Ullah Riaz, Mahmood Hafiz Majid, Ali Syed Saeed, Ibenmoussa Samir, Brahim Admou
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	systemic autoimmune diseases antinuclear antibodies immunoblot immunodot indirect immunofluorescence Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d68f5789f83e40d495587ea21a97978d
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spelling	oai:doaj.org-article:d68f5789f83e40d495587ea21a97978d2021-12-05T14:10:43ZEvaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases2391-542010.1515/chem-2020-0101https://doaj.org/article/d68f5789f83e40d495587ea21a97978d2021-03-01T00:00:00Zhttps://doi.org/10.1515/chem-2020-0101https://doaj.org/toc/2391-5420Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were tested positive for antinuclear antibodies. The patients were initially screened for antinuclear antibodies using indirect immunofluorescence. Then, the identification of specific autoantibodies such as anti-extractable nuclear antigens (ENAs) was carried out using the immunoblot and immunodot techniques. The results showed that immunoblot and immunodot did not present a significant difference in their sensitivity against anti-SSA/52, SSB, CENP-B, PCNA, U1-snRNP, Jo-1, Pm-scl, and Mi-2 (p > 0.05). However, the two techniques showed a significant difference in their sensitivity toward autoantibodies anti-DNAn, anti-histone, anti-SmD1, and anti-ds-DNA (p < 0.05). The immunoblot data were in complete accordance with the immunodot data (100%) regarding the detection of autoantibodies such as anti SSA/52, SSB, CENP-B, PCNA, U1-snRP, Jo-1, Pm-scl, and Mi-2, 80% regarding SmD1, and 75% concerning ds-DNA. We should certainly pay closer attention to the efficiency of the techniques used in the diagnosis of autoimmune diseases.EL Hassouni YoussefBourhia MohammedBari AhmedUllah RiazMahmood Hafiz MajidAli Syed SaeedIbenmoussa SamirBrahim AdmouDe Gruyterarticlesystemic autoimmune diseasesantinuclear antibodiesimmunoblotimmunodotindirect immunofluorescenceChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 237-244 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	systemic autoimmune diseases antinuclear antibodies immunoblot immunodot indirect immunofluorescence Chemistry QD1-999
spellingShingle	systemic autoimmune diseases antinuclear antibodies immunoblot immunodot indirect immunofluorescence Chemistry QD1-999 EL Hassouni Youssef Bourhia Mohammed Bari Ahmed Ullah Riaz Mahmood Hafiz Majid Ali Syed Saeed Ibenmoussa Samir Brahim Admou Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
description	Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were tested positive for antinuclear antibodies. The patients were initially screened for antinuclear antibodies using indirect immunofluorescence. Then, the identification of specific autoantibodies such as anti-extractable nuclear antigens (ENAs) was carried out using the immunoblot and immunodot techniques. The results showed that immunoblot and immunodot did not present a significant difference in their sensitivity against anti-SSA/52, SSB, CENP-B, PCNA, U1-snRNP, Jo-1, Pm-scl, and Mi-2 (p > 0.05). However, the two techniques showed a significant difference in their sensitivity toward autoantibodies anti-DNAn, anti-histone, anti-SmD1, and anti-ds-DNA (p < 0.05). The immunoblot data were in complete accordance with the immunodot data (100%) regarding the detection of autoantibodies such as anti SSA/52, SSB, CENP-B, PCNA, U1-snRP, Jo-1, Pm-scl, and Mi-2, 80% regarding SmD1, and 75% concerning ds-DNA. We should certainly pay closer attention to the efficiency of the techniques used in the diagnosis of autoimmune diseases.
format	article
author	EL Hassouni Youssef Bourhia Mohammed Bari Ahmed Ullah Riaz Mahmood Hafiz Majid Ali Syed Saeed Ibenmoussa Samir Brahim Admou
author_facet	EL Hassouni Youssef Bourhia Mohammed Bari Ahmed Ullah Riaz Mahmood Hafiz Majid Ali Syed Saeed Ibenmoussa Samir Brahim Admou
author_sort	EL Hassouni Youssef
title	Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
title_short	Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
title_full	Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
title_fullStr	Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
title_full_unstemmed	Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
title_sort	evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/d68f5789f83e40d495587ea21a97978d
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Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases

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