Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans

Abstract LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional ‘LIM code’ that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Isle...

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Autores principales:	Mugdha Bhati, Estelle Llamosas, David A. Jacques, Cy M. Jeffries, Siavoush Dastmalchi, Nina Ripin, Hannah R. Nicholas, Jacqueline M. Matthews
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d69119c3e92447f7a819a9bdea605bf6
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spelling	oai:doaj.org-article:d69119c3e92447f7a819a9bdea605bf62021-12-02T16:06:46ZInteractions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans10.1038/s41598-017-04587-82045-2322https://doaj.org/article/d69119c3e92447f7a819a9bdea605bf62017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04587-8https://doaj.org/toc/2045-2322Abstract LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional ‘LIM code’ that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Islet1 (ISL1), is essential for the development of motor neurons. Using yeast two-hybrid analysis we showed that the Caenorhabditis elegans orthologs of LHX3 and ISL1, CEH-14 and LIM-7 can physically interact. Structural characterisation of a complex comprising the LIM domains from CEH-14 and a LIM-interaction domain from LIM-7 showed that these nematode proteins assemble to form a structure that closely resembles that of their vertebrate counterparts. However, mutagenic analysis across the interface indicates some differences in the mechanisms of binding. We also demonstrate, using fluorescent reporter constructs, that the two C. elegans proteins are co-expressed in a small subset of neurons. These data show that the propensity for LHX3 and Islet proteins to interact is conserved from C. elegans to mammals, raising the possibility that orthologous cell specific LIM-HD-containing transcription factor complexes play similar roles in the development of neuronal cells across diverse species.Mugdha BhatiEstelle LlamosasDavid A. JacquesCy M. JeffriesSiavoush DastmalchiNina RipinHannah R. NicholasJacqueline M. MatthewsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mugdha Bhati Estelle Llamosas David A. Jacques Cy M. Jeffries Siavoush Dastmalchi Nina Ripin Hannah R. Nicholas Jacqueline M. Matthews Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
description	Abstract LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional ‘LIM code’ that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Islet1 (ISL1), is essential for the development of motor neurons. Using yeast two-hybrid analysis we showed that the Caenorhabditis elegans orthologs of LHX3 and ISL1, CEH-14 and LIM-7 can physically interact. Structural characterisation of a complex comprising the LIM domains from CEH-14 and a LIM-interaction domain from LIM-7 showed that these nematode proteins assemble to form a structure that closely resembles that of their vertebrate counterparts. However, mutagenic analysis across the interface indicates some differences in the mechanisms of binding. We also demonstrate, using fluorescent reporter constructs, that the two C. elegans proteins are co-expressed in a small subset of neurons. These data show that the propensity for LHX3 and Islet proteins to interact is conserved from C. elegans to mammals, raising the possibility that orthologous cell specific LIM-HD-containing transcription factor complexes play similar roles in the development of neuronal cells across diverse species.
format	article
author	Mugdha Bhati Estelle Llamosas David A. Jacques Cy M. Jeffries Siavoush Dastmalchi Nina Ripin Hannah R. Nicholas Jacqueline M. Matthews
author_facet	Mugdha Bhati Estelle Llamosas David A. Jacques Cy M. Jeffries Siavoush Dastmalchi Nina Ripin Hannah R. Nicholas Jacqueline M. Matthews
author_sort	Mugdha Bhati
title	Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
title_short	Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
title_full	Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
title_fullStr	Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
title_full_unstemmed	Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
title_sort	interactions between lhx3- and isl1-family lim-homeodomain transcription factors are conserved in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d69119c3e92447f7a819a9bdea605bf6
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Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans

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