ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN

In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examine...

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Autores principales:	A. A. Lebedenko, T. P. Shkurat, E. V. Mashkina, O. E. Semernik, T. K. Dreyzina, E. B. Tyurina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	asthma genetics children diagnostics metalloproteinase gene polymorphism Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d69b467f45254e98b75735dcb3687d72
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spelling	oai:doaj.org-article:d69b467f45254e98b75735dcb3687d722021-11-18T08:03:47ZASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN1563-06252313-741X10.15789/1563-0625-2018-6-905-912https://doaj.org/article/d69b467f45254e98b75735dcb3687d722018-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1678https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XIn the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease.A. A. LebedenkoT. P. ShkuratE. V. MashkinaO. E. SemernikT. K. DreyzinaE. B. TyurinaSPb RAACIarticleasthmageneticschildrendiagnosticsmetalloproteinasegene polymorphismImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 6, Pp 905-912 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	asthma genetics children diagnostics metalloproteinase gene polymorphism Immunologic diseases. Allergy RC581-607
spellingShingle	asthma genetics children diagnostics metalloproteinase gene polymorphism Immunologic diseases. Allergy RC581-607 A. A. Lebedenko T. P. Shkurat E. V. Mashkina O. E. Semernik T. K. Dreyzina E. B. Tyurina ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
description	In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease.
format	article
author	A. A. Lebedenko T. P. Shkurat E. V. Mashkina O. E. Semernik T. K. Dreyzina E. B. Tyurina
author_facet	A. A. Lebedenko T. P. Shkurat E. V. Mashkina O. E. Semernik T. K. Dreyzina E. B. Tyurina
author_sort	A. A. Lebedenko
title	ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
title_short	ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
title_full	ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
title_fullStr	ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
title_full_unstemmed	ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
title_sort	association of matrix metalloproteinases gene polymorphism with clinical manifestations of bronchial asthma in children
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/d69b467f45254e98b75735dcb3687d72
work_keys_str_mv	AT aalebedenko associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren AT tpshkurat associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren AT evmashkina associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren AT oesemernik associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren AT tkdreyzina associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren AT ebtyurina associationofmatrixmetalloproteinasesgenepolymorphismwithclinicalmanifestationsofbronchialasthmainchildren
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ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN

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