RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

Abstract Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a p...

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Autores principales:	Shulin Cao, Yi He, Chaofeng Hao, Yan Xu, Hongchang Zhang, Chenfang Wang, Huiquan Liu, Jin-Rong Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d69b9be944fb403697ef40b83ee4f189
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spelling	oai:doaj.org-article:d69b9be944fb403697ef40b83ee4f1892021-12-02T11:41:10ZRNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum10.1038/s41598-017-04960-72045-2322https://doaj.org/article/d69b9be944fb403697ef40b83ee4f1892017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04960-7https://doaj.org/toc/2045-2322Abstract Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a protein with a major facilitator superfamily (MFS) domain. Here, we characterized the functions of AMD1 and its UAG to UGG editing event. The amd1 deletion mutant was normal in growth and conidiation but defective in ascospore discharge due to the premature breakdown of its ascus wall in older perithecia, which is consistent with the specific expression of AMD1 at later stages of sexual development. Expression of the wild-type or edited allele of AMD1 but not un-editable allele rescued the defects of amd1 in ascospore discharge. Furthermore, Amd1-GFP localized to the ascus membrane and Amd1 orthologs are only present in ascocarp-forming fungi that physically discharge ascospores. Interestingly, deletion of AMD1 results in the up-regulation of a number of genes related to transporter activity and membrane functions. Overall, these results indicated that Amd1 may play a critical role in maintaining ascus wall integrity during ascus maturation, and A-to-I editing of its transcripts is important for ascospore discharge in F. graminearum.Shulin CaoYi HeChaofeng HaoYan XuHongchang ZhangChenfang WangHuiquan LiuJin-Rong XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shulin Cao Yi He Chaofeng Hao Yan Xu Hongchang Zhang Chenfang Wang Huiquan Liu Jin-Rong Xu RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
description	Abstract Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a protein with a major facilitator superfamily (MFS) domain. Here, we characterized the functions of AMD1 and its UAG to UGG editing event. The amd1 deletion mutant was normal in growth and conidiation but defective in ascospore discharge due to the premature breakdown of its ascus wall in older perithecia, which is consistent with the specific expression of AMD1 at later stages of sexual development. Expression of the wild-type or edited allele of AMD1 but not un-editable allele rescued the defects of amd1 in ascospore discharge. Furthermore, Amd1-GFP localized to the ascus membrane and Amd1 orthologs are only present in ascocarp-forming fungi that physically discharge ascospores. Interestingly, deletion of AMD1 results in the up-regulation of a number of genes related to transporter activity and membrane functions. Overall, these results indicated that Amd1 may play a critical role in maintaining ascus wall integrity during ascus maturation, and A-to-I editing of its transcripts is important for ascospore discharge in F. graminearum.
format	article
author	Shulin Cao Yi He Chaofeng Hao Yan Xu Hongchang Zhang Chenfang Wang Huiquan Liu Jin-Rong Xu
author_facet	Shulin Cao Yi He Chaofeng Hao Yan Xu Hongchang Zhang Chenfang Wang Huiquan Liu Jin-Rong Xu
author_sort	Shulin Cao
title	RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
title_short	RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
title_full	RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
title_fullStr	RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
title_full_unstemmed	RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum
title_sort	rna editing of the amd1 gene is important for ascus maturation and ascospore discharge in fusarium graminearum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d69b9be944fb403697ef40b83ee4f189
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RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

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