First report of elevated monocyte-platelet aggregates in healthy children.

Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggr...

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Autores principales:	Christina Yip, Vera Ignjatovic, Chantal Attard, Paul Monagle, Matthew D Linden
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6a0a8fc1fe347c4825c7ef001d0e1d7
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spelling	oai:doaj.org-article:d6a0a8fc1fe347c4825c7ef001d0e1d72021-11-18T07:40:17ZFirst report of elevated monocyte-platelet aggregates in healthy children.1932-620310.1371/journal.pone.0067416https://doaj.org/article/d6a0a8fc1fe347c4825c7ef001d0e1d72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826296/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children.Christina YipVera IgnjatovicChantal AttardPaul MonagleMatthew D LindenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67416 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christina Yip Vera Ignjatovic Chantal Attard Paul Monagle Matthew D Linden First report of elevated monocyte-platelet aggregates in healthy children.
description	Platelets are subcellular fragments which circulate in blood and have well established roles in thrombosis and haemostasis in adults. Upon activation, platelets undergo granule exocytosis and express P-Selectin on the cell membrane which binds a ligand on monocytes, leading to monocyte-platelet aggregation. Elevated circulating monocyte-platelet aggregates in adults are linked to atherothrombosis, but have not been investigated in children where thrombosis is less common. This study aimed to measure monocyte-platelet aggregate formation in children using whole blood flow cytometry. Monocyte-platelet aggregates as well as activation and granule exocytosis of platelets were measured in healthy adults (n = 15, median age 28 years) and healthy children (n = 28, median age 7 years). Monocyte-platelet aggregates in healthy children were elevated compared to healthy adults (37.8±4.4% vs 15.5±1.9% respectively, p<0.01). However, this was not accompanied by any difference in platelet activation (PAC-1 binding 6.8±1.5% vs 6.3±2.0% respectively, p = ns) or granule exocytosis (P-selectin expression 4.4±0.5% vs 3.1±0.5% respectively, p = ns). Despite comparable numbers of platelets bound per monocyte (GPIb MFI 117.3±13.7 vs 130.9±28.6 respectively, p = ns), surface P-selectin expression per platelet-bound monocyte was lower in children compared to adults. We therefore provide the first data of elevated monocyte-platelet aggregates in healthy children.
format	article
author	Christina Yip Vera Ignjatovic Chantal Attard Paul Monagle Matthew D Linden
author_facet	Christina Yip Vera Ignjatovic Chantal Attard Paul Monagle Matthew D Linden
author_sort	Christina Yip
title	First report of elevated monocyte-platelet aggregates in healthy children.
title_short	First report of elevated monocyte-platelet aggregates in healthy children.
title_full	First report of elevated monocyte-platelet aggregates in healthy children.
title_fullStr	First report of elevated monocyte-platelet aggregates in healthy children.
title_full_unstemmed	First report of elevated monocyte-platelet aggregates in healthy children.
title_sort	first report of elevated monocyte-platelet aggregates in healthy children.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d6a0a8fc1fe347c4825c7ef001d0e1d7
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First report of elevated monocyte-platelet aggregates in healthy children.

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