A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency

Abstract Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine and are frequently used in preclinical mouse models for both mechanistic studies and screening of new cell...

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Autores principales:	Courtney M. Caroti, Hyunhee Ahn, Hector F. Salazar, Giji Joseph, Sitara B. Sankar, Nick J. Willett, Levi B. Wood, W. Robert Taylor, Alicia N. Lyle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6a770956930470a9d617a16565719a9
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spelling	oai:doaj.org-article:d6a770956930470a9d617a16565719a92021-12-02T15:05:42ZA Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency10.1038/s41598-017-13477-y2045-2322https://doaj.org/article/d6a770956930470a9d617a16565719a92017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13477-yhttps://doaj.org/toc/2045-2322Abstract Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine and are frequently used in preclinical mouse models for both mechanistic studies and screening of new cell based therapies. Current methods to culture murine MSCs (mMSCs) select for rapidly dividing colonies and require long-term expansion. These methods thus require months of culture to generate sufficient cell numbers for feasibility studies in a lab setting and the cell populations often have reduced proliferation and differentiation potential, or have become immortalized cells. Here we describe a simple and reproducible method to generate mMSCs by utilizing hypoxia and basic fibroblast growth factor supplementation. Cells produced using these conditions were generated 2.8 times faster than under traditional methods and the mMSCs showed decreased senescence and maintained their multipotency and differentiation potential until passage 11 and beyond. Our method for mMSC isolation and expansion will significantly improve the utility of this critical cell source in pre-clinical studies for the investigation of MSC mechanisms, therapies, and cell manufacturing strategies.Courtney M. CarotiHyunhee AhnHector F. SalazarGiji JosephSitara B. SankarNick J. WillettLevi B. WoodW. Robert TaylorAlicia N. LyleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Courtney M. Caroti Hyunhee Ahn Hector F. Salazar Giji Joseph Sitara B. Sankar Nick J. Willett Levi B. Wood W. Robert Taylor Alicia N. Lyle A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
description	Abstract Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine and are frequently used in preclinical mouse models for both mechanistic studies and screening of new cell based therapies. Current methods to culture murine MSCs (mMSCs) select for rapidly dividing colonies and require long-term expansion. These methods thus require months of culture to generate sufficient cell numbers for feasibility studies in a lab setting and the cell populations often have reduced proliferation and differentiation potential, or have become immortalized cells. Here we describe a simple and reproducible method to generate mMSCs by utilizing hypoxia and basic fibroblast growth factor supplementation. Cells produced using these conditions were generated 2.8 times faster than under traditional methods and the mMSCs showed decreased senescence and maintained their multipotency and differentiation potential until passage 11 and beyond. Our method for mMSC isolation and expansion will significantly improve the utility of this critical cell source in pre-clinical studies for the investigation of MSC mechanisms, therapies, and cell manufacturing strategies.
format	article
author	Courtney M. Caroti Hyunhee Ahn Hector F. Salazar Giji Joseph Sitara B. Sankar Nick J. Willett Levi B. Wood W. Robert Taylor Alicia N. Lyle
author_facet	Courtney M. Caroti Hyunhee Ahn Hector F. Salazar Giji Joseph Sitara B. Sankar Nick J. Willett Levi B. Wood W. Robert Taylor Alicia N. Lyle
author_sort	Courtney M. Caroti
title	A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
title_short	A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
title_full	A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
title_fullStr	A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
title_full_unstemmed	A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency
title_sort	novel technique for accelerated culture of murine mesenchymal stem cells that allows for sustained multipotency
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d6a770956930470a9d617a16565719a9
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A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency

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