Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.

Hepatocellular carcinoma (HCC) is one of the most common cancer related deaths worldwide. One of the main challenges in cancer treatment is drug delivery to target cancer cells specifically. Preclinical evaluation of intratumoral drugs in orthotopic liver cancer mouse models is difficult, as percuta...

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Autores principales:	Gaétan Bour, Fernand Martel, Laurent Goffin, Bernard Bayle, Jacques Gangloff, Marc Aprahamian, Jacques Marescaux, Jean-Marc Egly
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6a7cad97c56439da39067b30ff15684
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spelling	oai:doaj.org-article:d6a7cad97c56439da39067b30ff156842021-11-25T06:01:15ZDesign and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.1932-620310.1371/journal.pone.0106675https://doaj.org/article/d6a7cad97c56439da39067b30ff156842014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106675https://doaj.org/toc/1932-6203Hepatocellular carcinoma (HCC) is one of the most common cancer related deaths worldwide. One of the main challenges in cancer treatment is drug delivery to target cancer cells specifically. Preclinical evaluation of intratumoral drugs in orthotopic liver cancer mouse models is difficult, as percutaneous injection hardly can be precisely performed manually. In the present study we have characterized a hepatoma model developing a single tumor nodule by implantation of Hep55.1C cells in the liver of syngeneic C57BL/6J mice. Tumor evolution was followed up by µCT imaging, and at the histological and molecular levels. This orthotopic, poorly differentiated mouse HCC model expressing fibrosis, inflammation and cancer markers was used to assess the efficacy of drugs. We took advantage of the high precision of a previously developed robotized system for automated, image-guided intratumoral needle insertion, to administer every week in the tumor of the Hep55.1C mouse model. A significant tumor growth inhibition was observed using our robotized system, whereas manual intraperitoneal administration had no effect, by comparison to untreated control mice.Gaétan BourFernand MartelLaurent GoffinBernard BayleJacques GangloffMarc AprahamianJacques MarescauxJean-Marc EglyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106675 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gaétan Bour Fernand Martel Laurent Goffin Bernard Bayle Jacques Gangloff Marc Aprahamian Jacques Marescaux Jean-Marc Egly Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
description	Hepatocellular carcinoma (HCC) is one of the most common cancer related deaths worldwide. One of the main challenges in cancer treatment is drug delivery to target cancer cells specifically. Preclinical evaluation of intratumoral drugs in orthotopic liver cancer mouse models is difficult, as percutaneous injection hardly can be precisely performed manually. In the present study we have characterized a hepatoma model developing a single tumor nodule by implantation of Hep55.1C cells in the liver of syngeneic C57BL/6J mice. Tumor evolution was followed up by µCT imaging, and at the histological and molecular levels. This orthotopic, poorly differentiated mouse HCC model expressing fibrosis, inflammation and cancer markers was used to assess the efficacy of drugs. We took advantage of the high precision of a previously developed robotized system for automated, image-guided intratumoral needle insertion, to administer every week in the tumor of the Hep55.1C mouse model. A significant tumor growth inhibition was observed using our robotized system, whereas manual intraperitoneal administration had no effect, by comparison to untreated control mice.
format	article
author	Gaétan Bour Fernand Martel Laurent Goffin Bernard Bayle Jacques Gangloff Marc Aprahamian Jacques Marescaux Jean-Marc Egly
author_facet	Gaétan Bour Fernand Martel Laurent Goffin Bernard Bayle Jacques Gangloff Marc Aprahamian Jacques Marescaux Jean-Marc Egly
author_sort	Gaétan Bour
title	Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
title_short	Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
title_full	Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
title_fullStr	Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
title_full_unstemmed	Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
title_sort	design and development of a robotized system coupled to µct imaging for intratumoral drug evaluation in a hcc mouse model.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d6a7cad97c56439da39067b30ff15684
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Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.

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