Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence

Abstract Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observ...

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Autores principales:	Christin Schneider, Lisa Gebhardt, Stephanie Arndt, Sigrid Karrer, Julia L. Zimmermann, Michael J. M. Fischer, Anja-Katrin Bosserhoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6ac0c68457e4130a56c318724b91166
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spelling	oai:doaj.org-article:d6ac0c68457e4130a56c318724b911662021-12-02T11:41:24ZCold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence10.1038/s41598-018-28443-52045-2322https://doaj.org/article/d6ac0c68457e4130a56c318724b911662018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28443-5https://doaj.org/toc/2045-2322Abstract Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observed increased cytoplasmic calcium after CAP treatment, which also occurred in the absence of extracellular calcium, indicating the majority of the calcium increase originates from intracellular stores. Application of previously CAP-exposed extracellular solutions also induced cytoplasmic calcium elevations. A substantial fraction of this effect remained when the application was delayed for one hour, indicating the chemical stability of the activating agent(s). Addition of ryanodine and cyclosporin A indicate the involvement of the endoplasmatic reticulum and the mitochondria. Inhibition of the cytoplasmic calcium elevation by the intracellular chelator BAPTA blocked CAP-induced senescence. This finding helps to understand the molecular influence and the mode of action of CAP on tumor cells.Christin SchneiderLisa GebhardtStephanie ArndtSigrid KarrerJulia L. ZimmermannMichael J. M. FischerAnja-Katrin BosserhoffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christin Schneider Lisa Gebhardt Stephanie Arndt Sigrid Karrer Julia L. Zimmermann Michael J. M. Fischer Anja-Katrin Bosserhoff Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
description	Abstract Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observed increased cytoplasmic calcium after CAP treatment, which also occurred in the absence of extracellular calcium, indicating the majority of the calcium increase originates from intracellular stores. Application of previously CAP-exposed extracellular solutions also induced cytoplasmic calcium elevations. A substantial fraction of this effect remained when the application was delayed for one hour, indicating the chemical stability of the activating agent(s). Addition of ryanodine and cyclosporin A indicate the involvement of the endoplasmatic reticulum and the mitochondria. Inhibition of the cytoplasmic calcium elevation by the intracellular chelator BAPTA blocked CAP-induced senescence. This finding helps to understand the molecular influence and the mode of action of CAP on tumor cells.
format	article
author	Christin Schneider Lisa Gebhardt Stephanie Arndt Sigrid Karrer Julia L. Zimmermann Michael J. M. Fischer Anja-Katrin Bosserhoff
author_facet	Christin Schneider Lisa Gebhardt Stephanie Arndt Sigrid Karrer Julia L. Zimmermann Michael J. M. Fischer Anja-Katrin Bosserhoff
author_sort	Christin Schneider
title	Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
title_short	Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
title_full	Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
title_fullStr	Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
title_full_unstemmed	Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence
title_sort	cold atmospheric plasma causes a calcium influx in melanoma cells triggering cap-induced senescence
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d6ac0c68457e4130a56c318724b91166
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Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence

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