Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.

In both the pharmaceutical and agricultural fields, RNA-based products have capitalized upon the mechanism of RNA interference for targeted reduction of gene expression to improve phenotypes and traits. Reduction in gene expression by RNAi is the result of a small interfering RNA (siRNA) molecule bi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peizhen Yang, Ericka Havecker, Matthew Bauer, Carl Diehl, Bill Hendrix, Paul Hoffer, Timothy Boyle, John Bradley, Amy Caruano-Yzermans, Jill Deikman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6b92fba9dea4747ac97c7f203ecb861
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d6b92fba9dea4747ac97c7f203ecb861
record_format	dspace
spelling	oai:doaj.org-article:d6b92fba9dea4747ac97c7f203ecb8612021-12-02T20:08:28ZBeyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.1932-620310.1371/journal.pone.0256863https://doaj.org/article/d6b92fba9dea4747ac97c7f203ecb8612021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256863https://doaj.org/toc/1932-6203In both the pharmaceutical and agricultural fields, RNA-based products have capitalized upon the mechanism of RNA interference for targeted reduction of gene expression to improve phenotypes and traits. Reduction in gene expression by RNAi is the result of a small interfering RNA (siRNA) molecule binding to an ARGONAUTE (AGO) protein and directing the effector complex to a homologous region of a target gene's mRNA. siRNAs properties that govern RNA-AGO association have been studied in detail. The siRNA 5' nucleotide (nt) identity has been demonstrated in plants to be an important property responsible for directing association of endogenous small RNAs with different AGO effector proteins. However, it has not been investigated whether the 5' nt identity is an efficacious determinant for topically-applied chemically synthesized siRNAs. In this study, we employed a sandpaper abrasion method to study the silencing efficacies of topically-applied 21 base-pair siRNA duplexes. The MAGNESIUM CHELATASE and GREEN FLUORESCENT PROTEIN genes were selected as endogenous and transgenic gene targets, respectively, to assess the molecular and phenotypic effects of gene silencing. Collections of siRNA variants with different 5' nt identities and different pairing states between the 5' antisense nt and its match in the sense strand of the siRNA duplex were tested for their silencing efficacy. Our results suggest a flexibility in the 5' nt requirement for topically applied siRNA duplexes in planta and highlight the similarity of 5' thermodynamic rules governing topical siRNA efficacy across plants and animals.Peizhen YangEricka HaveckerMatthew BauerCarl DiehlBill HendrixPaul HofferTimothy BoyleJohn BradleyAmy Caruano-YzermansJill DeikmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256863 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peizhen Yang Ericka Havecker Matthew Bauer Carl Diehl Bill Hendrix Paul Hoffer Timothy Boyle John Bradley Amy Caruano-Yzermans Jill Deikman Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
description	In both the pharmaceutical and agricultural fields, RNA-based products have capitalized upon the mechanism of RNA interference for targeted reduction of gene expression to improve phenotypes and traits. Reduction in gene expression by RNAi is the result of a small interfering RNA (siRNA) molecule binding to an ARGONAUTE (AGO) protein and directing the effector complex to a homologous region of a target gene's mRNA. siRNAs properties that govern RNA-AGO association have been studied in detail. The siRNA 5' nucleotide (nt) identity has been demonstrated in plants to be an important property responsible for directing association of endogenous small RNAs with different AGO effector proteins. However, it has not been investigated whether the 5' nt identity is an efficacious determinant for topically-applied chemically synthesized siRNAs. In this study, we employed a sandpaper abrasion method to study the silencing efficacies of topically-applied 21 base-pair siRNA duplexes. The MAGNESIUM CHELATASE and GREEN FLUORESCENT PROTEIN genes were selected as endogenous and transgenic gene targets, respectively, to assess the molecular and phenotypic effects of gene silencing. Collections of siRNA variants with different 5' nt identities and different pairing states between the 5' antisense nt and its match in the sense strand of the siRNA duplex were tested for their silencing efficacy. Our results suggest a flexibility in the 5' nt requirement for topically applied siRNA duplexes in planta and highlight the similarity of 5' thermodynamic rules governing topical siRNA efficacy across plants and animals.
format	article
author	Peizhen Yang Ericka Havecker Matthew Bauer Carl Diehl Bill Hendrix Paul Hoffer Timothy Boyle John Bradley Amy Caruano-Yzermans Jill Deikman
author_facet	Peizhen Yang Ericka Havecker Matthew Bauer Carl Diehl Bill Hendrix Paul Hoffer Timothy Boyle John Bradley Amy Caruano-Yzermans Jill Deikman
author_sort	Peizhen Yang
title	Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
title_short	Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
title_full	Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
title_fullStr	Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
title_full_unstemmed	Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.
title_sort	beyond identity: understanding the contribution of the 5' nucleotide of the antisense strand to rnai activity.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d6b92fba9dea4747ac97c7f203ecb861
work_keys_str_mv	AT peizhenyang beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT erickahavecker beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT matthewbauer beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT carldiehl beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT billhendrix beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT paulhoffer beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT timothyboyle beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT johnbradley beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT amycaruanoyzermans beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity AT jilldeikman beyondidentityunderstandingthecontributionofthe5nucleotideoftheantisensestrandtornaiactivity
_version_	1718375211161616384

Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.

Ejemplares similares