Molecular typing of Rickettsia akari

Rickettsia akari, an obligately intracellular bacterium, is the causative agent of the cosmopolitan urban disease rickettsialpox. R. akari is an atypical representative of spotted fever group rickettsiae (SFG) as it is associated with rodent mites rather than ticks or fleas; however, only limited in...

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Autores principales:	M. E. Eremeeva, M. M. Sturgeon, J. K. Willard, S. E. Karpathy, A. Madan, G. A. Dasch
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	rickettsialpox mite liponyssoides sanguineus rickettsia akari rickettsiae spotted fever group rickettsiae tandem repeats intergenic regions genotyping multilocus sequence typing Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/d6c12dac50ef440fbbbc630ff788a041
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spelling	oai:doaj.org-article:d6c12dac50ef440fbbbc630ff788a0412021-11-22T07:09:54ZMolecular typing of Rickettsia akari2220-76192313-739810.15789/2220-7619-MTO-1295https://doaj.org/article/d6c12dac50ef440fbbbc630ff788a0412020-08-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1295https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Rickettsia akari, an obligately intracellular bacterium, is the causative agent of the cosmopolitan urban disease rickettsialpox. R. akari is an atypical representative of spotted fever group rickettsiae (SFG) as it is associated with rodent mites rather than ticks or fleas; however, only limited information is available about the degree of genetic variability found among isolates of R. akari. We examined 13 isolates of R. akari from humans, rodents and mites in the USA, the former Soviet Union, and the former Yugoslavia made between 1946 and 2003 for diversity in their tandem repeat regions (TR) and intergenic regions (IGR). The 1.23 Mb genome of R. akari strain Hartford CWPP was analyzed using Tandem Repeat Finder software (http://tandem.bu.edu) and 374 different TRs were identified, with size variation from 1 to 483 bp and with TR copy numbers ranging between 21 and 1.9, respectively. No size polymorphisms were detected among the 11 TR regions examined from 5 open reading frames and 6 IGR. Eighteen non-TR IGR’s were amplified and sequenced for the same isolates comprising a total of 5.995 bp (0.49%) of the Hartford CWPP strain chromosome. Three single nucleotide polymorphism (SNP) sites were detected in two IGR’s which permitted separation of the five R. akari isolates from Ukraine SSR from the other eight isolates. In conclusion, this is the first study reporting genetic heterogeneity among R. akari isolates of different geographic origins. Further exploration of this genetic diversity is needed to understand better the geographic distribution of R. akari and the epidemiology of rickettsialpox. The potential of mites as hosts for other rickettsial agents also needs further investigation.M. E. EremeevaM. M. SturgeonJ. K. WillardS. E. KarpathyA. MadanG. A. DaschSankt-Peterburg : NIIÈM imeni Pasteraarticlerickettsialpoxmiteliponyssoides sanguineusrickettsia akaririckettsiaespotted fever group rickettsiaetandem repeatsintergenic regionsgenotypingmultilocus sequence typingInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 3, Pp 497-505 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	rickettsialpox mite liponyssoides sanguineus rickettsia akari rickettsiae spotted fever group rickettsiae tandem repeats intergenic regions genotyping multilocus sequence typing Infectious and parasitic diseases RC109-216
spellingShingle	rickettsialpox mite liponyssoides sanguineus rickettsia akari rickettsiae spotted fever group rickettsiae tandem repeats intergenic regions genotyping multilocus sequence typing Infectious and parasitic diseases RC109-216 M. E. Eremeeva M. M. Sturgeon J. K. Willard S. E. Karpathy A. Madan G. A. Dasch Molecular typing of Rickettsia akari
description	Rickettsia akari, an obligately intracellular bacterium, is the causative agent of the cosmopolitan urban disease rickettsialpox. R. akari is an atypical representative of spotted fever group rickettsiae (SFG) as it is associated with rodent mites rather than ticks or fleas; however, only limited information is available about the degree of genetic variability found among isolates of R. akari. We examined 13 isolates of R. akari from humans, rodents and mites in the USA, the former Soviet Union, and the former Yugoslavia made between 1946 and 2003 for diversity in their tandem repeat regions (TR) and intergenic regions (IGR). The 1.23 Mb genome of R. akari strain Hartford CWPP was analyzed using Tandem Repeat Finder software (http://tandem.bu.edu) and 374 different TRs were identified, with size variation from 1 to 483 bp and with TR copy numbers ranging between 21 and 1.9, respectively. No size polymorphisms were detected among the 11 TR regions examined from 5 open reading frames and 6 IGR. Eighteen non-TR IGR’s were amplified and sequenced for the same isolates comprising a total of 5.995 bp (0.49%) of the Hartford CWPP strain chromosome. Three single nucleotide polymorphism (SNP) sites were detected in two IGR’s which permitted separation of the five R. akari isolates from Ukraine SSR from the other eight isolates. In conclusion, this is the first study reporting genetic heterogeneity among R. akari isolates of different geographic origins. Further exploration of this genetic diversity is needed to understand better the geographic distribution of R. akari and the epidemiology of rickettsialpox. The potential of mites as hosts for other rickettsial agents also needs further investigation.
format	article
author	M. E. Eremeeva M. M. Sturgeon J. K. Willard S. E. Karpathy A. Madan G. A. Dasch
author_facet	M. E. Eremeeva M. M. Sturgeon J. K. Willard S. E. Karpathy A. Madan G. A. Dasch
author_sort	M. E. Eremeeva
title	Molecular typing of Rickettsia akari
title_short	Molecular typing of Rickettsia akari
title_full	Molecular typing of Rickettsia akari
title_fullStr	Molecular typing of Rickettsia akari
title_full_unstemmed	Molecular typing of Rickettsia akari
title_sort	molecular typing of rickettsia akari
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/d6c12dac50ef440fbbbc630ff788a041
work_keys_str_mv	AT meeremeeva moleculartypingofrickettsiaakari AT mmsturgeon moleculartypingofrickettsiaakari AT jkwillard moleculartypingofrickettsiaakari AT sekarpathy moleculartypingofrickettsiaakari AT amadan moleculartypingofrickettsiaakari AT gadasch moleculartypingofrickettsiaakari
_version_	1718417946521370624

Molecular typing of Rickettsia akari

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