Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19
Abstract Gastrointestinal symptoms are common in COVID-19 patients but the nature of the gut immune response to SARS-CoV-2 remains poorly characterized, partly due to the difficulty of obtaining biopsy specimens from infected individuals. In lieu of tissue samples, we measured cytokines, inflammator...
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Nature Portfolio
2021
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oai:doaj.org-article:d6c31edc6052419cbb41e75506d8cb062021-12-02T17:45:03ZLimited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-1910.1038/s41598-021-92740-92045-2322https://doaj.org/article/d6c31edc6052419cbb41e75506d8cb062021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92740-9https://doaj.org/toc/2045-2322Abstract Gastrointestinal symptoms are common in COVID-19 patients but the nature of the gut immune response to SARS-CoV-2 remains poorly characterized, partly due to the difficulty of obtaining biopsy specimens from infected individuals. In lieu of tissue samples, we measured cytokines, inflammatory markers, viral RNA, microbiome composition, and antibody responses in stool samples from a cohort of 44 hospitalized COVID-19 patients. SARS-CoV-2 RNA was detected in stool of 41% of patients and more frequently in patients with diarrhea. Patients who survived had lower fecal viral RNA than those who died. Strains isolated from stool and nasopharynx of an individual were the same. Compared to uninfected controls, COVID-19 patients had higher fecal levels of IL-8 and lower levels of fecal IL-10. Stool IL-23 was higher in patients with more severe COVID-19 disease, and we found evidence of intestinal virus-specific IgA responses associated with more severe disease. We provide evidence for an ongoing humeral immune response to SARS-CoV-2 in the gastrointestinal tract, but little evidence of overt inflammation.Graham J. BrittonAlice Chen-LiawFrancesca CossariniAlexandra E. LivanosMatthew P. SpindlerTamar PlittJoseph EggersIlaria MognoAna S. Gonzalez-ReicheSophia SiuMichael TankelevichLauren Tal GrinspanRebekah E. DixonDivya JhaAdriana van de GuchteZenab KhanGustavo Martinez-DelgadoFatima AmanatDaisy A. HoaglandBenjamin R. tenOeverMarla C. DubinskyMiriam MeradHarm van BakelFlorian KrammerGerold BongersSaurabh MehandruJeremiah J. FaithNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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DOAJ |
| collection |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Graham J. Britton Alice Chen-Liaw Francesca Cossarini Alexandra E. Livanos Matthew P. Spindler Tamar Plitt Joseph Eggers Ilaria Mogno Ana S. Gonzalez-Reiche Sophia Siu Michael Tankelevich Lauren Tal Grinspan Rebekah E. Dixon Divya Jha Adriana van de Guchte Zenab Khan Gustavo Martinez-Delgado Fatima Amanat Daisy A. Hoagland Benjamin R. tenOever Marla C. Dubinsky Miriam Merad Harm van Bakel Florian Krammer Gerold Bongers Saurabh Mehandru Jeremiah J. Faith Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| description |
Abstract Gastrointestinal symptoms are common in COVID-19 patients but the nature of the gut immune response to SARS-CoV-2 remains poorly characterized, partly due to the difficulty of obtaining biopsy specimens from infected individuals. In lieu of tissue samples, we measured cytokines, inflammatory markers, viral RNA, microbiome composition, and antibody responses in stool samples from a cohort of 44 hospitalized COVID-19 patients. SARS-CoV-2 RNA was detected in stool of 41% of patients and more frequently in patients with diarrhea. Patients who survived had lower fecal viral RNA than those who died. Strains isolated from stool and nasopharynx of an individual were the same. Compared to uninfected controls, COVID-19 patients had higher fecal levels of IL-8 and lower levels of fecal IL-10. Stool IL-23 was higher in patients with more severe COVID-19 disease, and we found evidence of intestinal virus-specific IgA responses associated with more severe disease. We provide evidence for an ongoing humeral immune response to SARS-CoV-2 in the gastrointestinal tract, but little evidence of overt inflammation. |
| format |
article |
| author |
Graham J. Britton Alice Chen-Liaw Francesca Cossarini Alexandra E. Livanos Matthew P. Spindler Tamar Plitt Joseph Eggers Ilaria Mogno Ana S. Gonzalez-Reiche Sophia Siu Michael Tankelevich Lauren Tal Grinspan Rebekah E. Dixon Divya Jha Adriana van de Guchte Zenab Khan Gustavo Martinez-Delgado Fatima Amanat Daisy A. Hoagland Benjamin R. tenOever Marla C. Dubinsky Miriam Merad Harm van Bakel Florian Krammer Gerold Bongers Saurabh Mehandru Jeremiah J. Faith |
| author_facet |
Graham J. Britton Alice Chen-Liaw Francesca Cossarini Alexandra E. Livanos Matthew P. Spindler Tamar Plitt Joseph Eggers Ilaria Mogno Ana S. Gonzalez-Reiche Sophia Siu Michael Tankelevich Lauren Tal Grinspan Rebekah E. Dixon Divya Jha Adriana van de Guchte Zenab Khan Gustavo Martinez-Delgado Fatima Amanat Daisy A. Hoagland Benjamin R. tenOever Marla C. Dubinsky Miriam Merad Harm van Bakel Florian Krammer Gerold Bongers Saurabh Mehandru Jeremiah J. Faith |
| author_sort |
Graham J. Britton |
| title |
Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| title_short |
Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| title_full |
Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| title_fullStr |
Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| title_full_unstemmed |
Limited intestinal inflammation despite diarrhea, fecal viral RNA and SARS-CoV-2-specific IgA in patients with acute COVID-19 |
| title_sort |
limited intestinal inflammation despite diarrhea, fecal viral rna and sars-cov-2-specific iga in patients with acute covid-19 |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/d6c31edc6052419cbb41e75506d8cb06 |
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