New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.

Chromodomains are found in many regulators of chromatin structure, and most of them recognize methylated lysines on histones. Here, we investigate the role of the Drosophila melanogaster protein Corto's chromodomain. The Enhancer of Trithorax and Polycomb Corto is involved in both silencing and...

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Autores principales:	Anne Coléno-Costes, Suk Min Jang, Augustin de Vanssay, Julien Rougeot, Tahar Bouceba, Neel B Randsholt, Jean-Michel Gibert, Stéphane Le Crom, Emmanuèle Mouchel-Vielh, Sébastien Bloyer, Frédérique Peronnet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d6c5bd326447471bbebd43f4c6e1b0e4
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spelling	oai:doaj.org-article:d6c5bd326447471bbebd43f4c6e1b0e42021-11-18T06:20:38ZNew partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.1553-73901553-740410.1371/journal.pgen.1003006https://doaj.org/article/d6c5bd326447471bbebd43f4c6e1b0e42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23071455/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Chromodomains are found in many regulators of chromatin structure, and most of them recognize methylated lysines on histones. Here, we investigate the role of the Drosophila melanogaster protein Corto's chromodomain. The Enhancer of Trithorax and Polycomb Corto is involved in both silencing and activation of gene expression. Over-expression of the Corto chromodomain (CortoCD) in transgenic flies shows that it is a chromatin-targeting module, critical for Corto function. Unexpectedly, mass spectrometry analysis reveals that polypeptides pulled down by CortoCD from nuclear extracts correspond to ribosomal proteins. Furthermore, real-time interaction analyses demonstrate that CortoCD binds with high affinity RPL12 tri-methylated on lysine 3. Corto and RPL12 co-localize with active epigenetic marks on polytene chromosomes, suggesting that both are involved in fine-tuning transcription of genes in open chromatin. RNA-seq based transcriptomes of wing imaginal discs over-expressing either CortoCD or RPL12 reveal that both factors deregulate large sets of common genes, which are enriched in heat-response and ribosomal protein genes, suggesting that they could be implicated in dynamic coordination of ribosome biogenesis. Chromatin immunoprecipitation experiments show that Corto and RPL12 bind hsp70 and are similarly recruited on gene body after heat shock. Hence, Corto and RPL12 could be involved together in regulation of gene transcription. We discuss whether pseudo-ribosomal complexes composed of various ribosomal proteins might participate in regulation of gene expression in connection with chromatin regulators.Anne Coléno-CostesSuk Min JangAugustin de VanssayJulien RougeotTahar BoucebaNeel B RandsholtJean-Michel GibertStéphane Le CromEmmanuèle Mouchel-VielhSébastien BloyerFrédérique PeronnetPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 8, Iss 10, p e1003006 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Anne Coléno-Costes Suk Min Jang Augustin de Vanssay Julien Rougeot Tahar Bouceba Neel B Randsholt Jean-Michel Gibert Stéphane Le Crom Emmanuèle Mouchel-Vielh Sébastien Bloyer Frédérique Peronnet New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
description	Chromodomains are found in many regulators of chromatin structure, and most of them recognize methylated lysines on histones. Here, we investigate the role of the Drosophila melanogaster protein Corto's chromodomain. The Enhancer of Trithorax and Polycomb Corto is involved in both silencing and activation of gene expression. Over-expression of the Corto chromodomain (CortoCD) in transgenic flies shows that it is a chromatin-targeting module, critical for Corto function. Unexpectedly, mass spectrometry analysis reveals that polypeptides pulled down by CortoCD from nuclear extracts correspond to ribosomal proteins. Furthermore, real-time interaction analyses demonstrate that CortoCD binds with high affinity RPL12 tri-methylated on lysine 3. Corto and RPL12 co-localize with active epigenetic marks on polytene chromosomes, suggesting that both are involved in fine-tuning transcription of genes in open chromatin. RNA-seq based transcriptomes of wing imaginal discs over-expressing either CortoCD or RPL12 reveal that both factors deregulate large sets of common genes, which are enriched in heat-response and ribosomal protein genes, suggesting that they could be implicated in dynamic coordination of ribosome biogenesis. Chromatin immunoprecipitation experiments show that Corto and RPL12 bind hsp70 and are similarly recruited on gene body after heat shock. Hence, Corto and RPL12 could be involved together in regulation of gene transcription. We discuss whether pseudo-ribosomal complexes composed of various ribosomal proteins might participate in regulation of gene expression in connection with chromatin regulators.
format	article
author	Anne Coléno-Costes Suk Min Jang Augustin de Vanssay Julien Rougeot Tahar Bouceba Neel B Randsholt Jean-Michel Gibert Stéphane Le Crom Emmanuèle Mouchel-Vielh Sébastien Bloyer Frédérique Peronnet
author_facet	Anne Coléno-Costes Suk Min Jang Augustin de Vanssay Julien Rougeot Tahar Bouceba Neel B Randsholt Jean-Michel Gibert Stéphane Le Crom Emmanuèle Mouchel-Vielh Sébastien Bloyer Frédérique Peronnet
author_sort	Anne Coléno-Costes
title	New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
title_short	New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
title_full	New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
title_fullStr	New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
title_full_unstemmed	New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.
title_sort	new partners in regulation of gene expression: the enhancer of trithorax and polycomb corto interacts with methylated ribosomal protein l12 via its chromodomain.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d6c5bd326447471bbebd43f4c6e1b0e4
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New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.

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