Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.

Data on the distribution and genotype of Cryptosporidium species is limited in Ethiopia. This study examined the presence and genetic diversity of Cryptosporidium species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) he...

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Autores principales:	Ambachew W Hailu, Abraham Degarege, Haileeyesus Adamu, Damien Costa, Venceslas Villier, Abdelmounaim Mouhajir, Loic Favennec, Romy Razakandrainibe, Beyene Petros
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6c6a1f0eacc4d439ee93a17eb4ad971
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spelling	oai:doaj.org-article:d6c6a1f0eacc4d439ee93a17eb4ad9712021-12-02T20:10:43ZMolecular characterization of Cryptosporidium spp. from humans in Ethiopia.1932-620310.1371/journal.pone.0253186https://doaj.org/article/d6c6a1f0eacc4d439ee93a17eb4ad9712021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253186https://doaj.org/toc/1932-6203Data on the distribution and genotype of Cryptosporidium species is limited in Ethiopia. This study examined the presence and genetic diversity of Cryptosporidium species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) health centers in Wurgissa and Hawassa district, respectively, were examined for the presence of Cryptosporidium spp. using microscopy, nested PCR and real-time PCR. To detect infection with PCR, analysis of 18S ribosomal RNA was performed. Subtyping was performed by sequencing a fragment of GP60 gene. The overall prevalence of infection was 46% (n = 86) by microscope and PCR. When 48 (out of 86) PCR positive samples were genotyped, two species were identified: C. parvum (n = 40) and C. hominis (n = 8). When 15 of the 40 C. parvum isolates were subtyped, zoonotic subtypes of IIaA14G1R1 (n = 1), IIaA15G2R1 (n = 1), IIaA16G1R1 (n = 2), IIaA16G3R1 (n = 2), IIaA17G1R1 (n = 1), IIaA19G1R1 (n = 1), IIaA20G1R1 (n = 3), IIaA22G1R1 (n = 1), IIaA22G2R1 (n = 1), IIdA23G1 (n = 1) and IIdA24G1 (n = 1) were identified. When 6 of the 8 C. hominis isolates were subtyped, subtypes IaA20 (n = 5), and IdA21(n = 1) were identified. This study suggests that C. parvum and C. hominis are causes of cryptosporidiosis in human in the Wurgissa district and Hawassa in Ethiopia. Zoonotic transmission might be the main route of transmission.Ambachew W HailuAbraham DegaregeHaileeyesus AdamuDamien CostaVenceslas VillierAbdelmounaim MouhajirLoic FavennecRomy RazakandrainibeBeyene PetrosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253186 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ambachew W Hailu Abraham Degarege Haileeyesus Adamu Damien Costa Venceslas Villier Abdelmounaim Mouhajir Loic Favennec Romy Razakandrainibe Beyene Petros Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
description	Data on the distribution and genotype of Cryptosporidium species is limited in Ethiopia. This study examined the presence and genetic diversity of Cryptosporidium species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) health centers in Wurgissa and Hawassa district, respectively, were examined for the presence of Cryptosporidium spp. using microscopy, nested PCR and real-time PCR. To detect infection with PCR, analysis of 18S ribosomal RNA was performed. Subtyping was performed by sequencing a fragment of GP60 gene. The overall prevalence of infection was 46% (n = 86) by microscope and PCR. When 48 (out of 86) PCR positive samples were genotyped, two species were identified: C. parvum (n = 40) and C. hominis (n = 8). When 15 of the 40 C. parvum isolates were subtyped, zoonotic subtypes of IIaA14G1R1 (n = 1), IIaA15G2R1 (n = 1), IIaA16G1R1 (n = 2), IIaA16G3R1 (n = 2), IIaA17G1R1 (n = 1), IIaA19G1R1 (n = 1), IIaA20G1R1 (n = 3), IIaA22G1R1 (n = 1), IIaA22G2R1 (n = 1), IIdA23G1 (n = 1) and IIdA24G1 (n = 1) were identified. When 6 of the 8 C. hominis isolates were subtyped, subtypes IaA20 (n = 5), and IdA21(n = 1) were identified. This study suggests that C. parvum and C. hominis are causes of cryptosporidiosis in human in the Wurgissa district and Hawassa in Ethiopia. Zoonotic transmission might be the main route of transmission.
format	article
author	Ambachew W Hailu Abraham Degarege Haileeyesus Adamu Damien Costa Venceslas Villier Abdelmounaim Mouhajir Loic Favennec Romy Razakandrainibe Beyene Petros
author_facet	Ambachew W Hailu Abraham Degarege Haileeyesus Adamu Damien Costa Venceslas Villier Abdelmounaim Mouhajir Loic Favennec Romy Razakandrainibe Beyene Petros
author_sort	Ambachew W Hailu
title	Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
title_short	Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
title_full	Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
title_fullStr	Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
title_full_unstemmed	Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.
title_sort	molecular characterization of cryptosporidium spp. from humans in ethiopia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d6c6a1f0eacc4d439ee93a17eb4ad971
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Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.

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