Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

Abstract Dentin is primarily composed of hydroxyapatite crystals within a rich organic matrix. The organic matrix comprises collagenous structural components, within which a variety of bioactive molecules are sequestered. During caries progression, dentin is degraded by acids and enzymes derived fro...

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Autores principales:	Motoki Okamoto, Yusuke Takahashi, Shungo Komichi, Paul R. Cooper, Mikako Hayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6c77b640fd74a27a29c8c8a195254e9
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spelling	oai:doaj.org-article:d6c77b640fd74a27a29c8c8a195254e92021-12-02T11:41:14ZDentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases10.1038/s41598-018-29112-32045-2322https://doaj.org/article/d6c77b640fd74a27a29c8c8a195254e92018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29112-3https://doaj.org/toc/2045-2322Abstract Dentin is primarily composed of hydroxyapatite crystals within a rich organic matrix. The organic matrix comprises collagenous structural components, within which a variety of bioactive molecules are sequestered. During caries progression, dentin is degraded by acids and enzymes derived from various sources, which can release bioactive molecules with potential reparative activity towards the dentin-pulp complex. While these molecules’ repair activities in other tissues are already known, their biological effects are unclear in relation to degradation events during disease in the dentin-pulp complex. This study was undertaken to investigate the effects of dentin matrix components (DMCs) that are partially digested by matrix metalloproteinases (MMPs) in vitro and in vivo during wound healing of the dentin-pulp complex. DMCs were initially isolated from healthy dentin and treated with recombinant MMPs. Subsequently, their effects on the behaviour of primary pulp cells were investigated in vitro and in vivo. Digested DMCs modulated a range of pulp cell functions in vitro. In addition, DMCs partially digested with MMP-20 stimulated tertiary dentin formation in vivo, which exhibited a more regular tubular structure than that induced by treatment with other MMPs. Our results indicate that MMP-20 may be especially effective in stimulating wound healing of the dentin-pulp complex.Motoki OkamotoYusuke TakahashiShungo KomichiPaul R. CooperMikako HayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-17 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Motoki Okamoto Yusuke Takahashi Shungo Komichi Paul R. Cooper Mikako Hayashi Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
description	Abstract Dentin is primarily composed of hydroxyapatite crystals within a rich organic matrix. The organic matrix comprises collagenous structural components, within which a variety of bioactive molecules are sequestered. During caries progression, dentin is degraded by acids and enzymes derived from various sources, which can release bioactive molecules with potential reparative activity towards the dentin-pulp complex. While these molecules’ repair activities in other tissues are already known, their biological effects are unclear in relation to degradation events during disease in the dentin-pulp complex. This study was undertaken to investigate the effects of dentin matrix components (DMCs) that are partially digested by matrix metalloproteinases (MMPs) in vitro and in vivo during wound healing of the dentin-pulp complex. DMCs were initially isolated from healthy dentin and treated with recombinant MMPs. Subsequently, their effects on the behaviour of primary pulp cells were investigated in vitro and in vivo. Digested DMCs modulated a range of pulp cell functions in vitro. In addition, DMCs partially digested with MMP-20 stimulated tertiary dentin formation in vivo, which exhibited a more regular tubular structure than that induced by treatment with other MMPs. Our results indicate that MMP-20 may be especially effective in stimulating wound healing of the dentin-pulp complex.
format	article
author	Motoki Okamoto Yusuke Takahashi Shungo Komichi Paul R. Cooper Mikako Hayashi
author_facet	Motoki Okamoto Yusuke Takahashi Shungo Komichi Paul R. Cooper Mikako Hayashi
author_sort	Motoki Okamoto
title	Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
title_short	Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
title_full	Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
title_fullStr	Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
title_full_unstemmed	Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
title_sort	dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d6c77b640fd74a27a29c8c8a195254e9
work_keys_str_mv	AT motokiokamoto dentinogeniceffectsofextracteddentinmatrixcomponentsdigestedwithmatrixmetalloproteinases AT yusuketakahashi dentinogeniceffectsofextracteddentinmatrixcomponentsdigestedwithmatrixmetalloproteinases AT shungokomichi dentinogeniceffectsofextracteddentinmatrixcomponentsdigestedwithmatrixmetalloproteinases AT paulrcooper dentinogeniceffectsofextracteddentinmatrixcomponentsdigestedwithmatrixmetalloproteinases AT mikakohayashi dentinogeniceffectsofextracteddentinmatrixcomponentsdigestedwithmatrixmetalloproteinases
_version_	1718395429951897600

Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

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