LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

Accumulating signs have found that long noncoding RNAs (lncRNAs) contribute to hepatocellular carcinoma (HCC). Here, we probed the effect and mechanism of lncRNA DARS-AS1 in HCC. The profiles of DARS-AS1 and Cytoskeleton associated protein 2 (CKAP2) in 50 HCC tissues and non-tumor tissues were exami...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanqing Feng, Gang Wei, Linfei Zhang, Huadong Zhou, Wei Wang, Peng Guo, Caitao Cheng, Lei Ji, Qinghe Cai, Yong Feng, Huahua Tu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	hepatocellular carcinoma dars-as1 ckap2 fak-erk pathway mir-3200-5p metastasis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d6cccd44fdbd499bb6c86084e32240d8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d6cccd44fdbd499bb6c86084e32240d8
record_format	dspace
spelling	oai:doaj.org-article:d6cccd44fdbd499bb6c86084e32240d82021-11-04T15:51:53ZLncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis2165-59792165-598710.1080/21655979.2021.1982272https://doaj.org/article/d6cccd44fdbd499bb6c86084e32240d82021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982272https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Accumulating signs have found that long noncoding RNAs (lncRNAs) contribute to hepatocellular carcinoma (HCC). Here, we probed the effect and mechanism of lncRNA DARS-AS1 in HCC. The profiles of DARS-AS1 and Cytoskeleton associated protein 2 (CKAP2) in 50 HCC tissues and non-tumor tissues were examined by real-time quantitative polymerase chain reaction (RT-qPCR). DARS-AS1 and CKAP2 overexpression and/or knockdown cell models were established. The proliferation, apoptosis, invasion and epithelial-mesenchymal transition (EMT) were determined. CKAP2, and focal adhesion kinase (FAK)-extracellular signal-regulated kinase (ERK) was tested by Western blot (WB). The relationship between DARS-AS1 and CKAP2 was predicted by Bioinformatics, and the dual-luciferase reporter assay was applied to verify the targeting association between miR-3200-5p and DARS-AS1 and CKAP2. DARS-AS1 was overexpressed in HCC tissues (vs. that in non-tumor tissues) and was closely correlated with the patients’ tumor stage. DARS-AS1 facilitated HCC cell proliferation and hampered apoptosis. HCC cell migration and EMT were enhanced by DARS-AS1. DARS-AS1 up-regulated CKAP2, which aggravated HCC. Further investigation illustrated that either DARS-AS1 or CKAP2 activated FAK-ERK pathway, and miR-3200-5p was competitively restrained by DARS-AS1. miR-3200-5p exerted tumor-suppressive effects in HCC and inactivated CKAP2 and FAK-ERK pathway. All in all, this study corroborates that DARS-AS1 facilitates HCC proliferation and metastasis by regulating miR-3200-5p-mediated CKAP2, which provides a potential target for HCC diagnosis and treatment. Abbreviations: CCK-8: cell counting kit-8; CKAP2: Cytoskeleton associated protein 2; cDNA:complementary DNA; DAPI: 4ʹ,6-diamidino-2-phenylindole; DARS-AS1: DARS1 antisense RNA 1; DEPC: diethyl pyrocarbonate; DMEM-F12: Dulbecco’s minimal essential medium/Ham’s-F12; EMT: epithelial-mesenchymal transition; ERK: extracellular signal-regulated kinase; FAK: focal adhesion kinase; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HCC: hepatocellular carcinoma; HE: hematoxylin-eosin; IHC: Immunohistochemistry; LIHC: Liver hepatocellular carcinoma; lncRNAs: long noncoding RNAs; MIAT: lncRNA myocardial infarction-related transcripts; MT: Mutant; NC: negative control; PBS: phosphate-buffered saline; PMSF: Phenylmethylsulfonyl fluoride; PVDF: polyvinylidene difluoride; RT: room temperature; RT-qPCR: real-time quantitative polymerase chain reaction; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SPF: specific pathogen-free; TMAP: tumor-associated microtubule-associated protein; TUNEL: TdT-mediated dUTP nick end labeling; V: volume; WT: wild type.Yanqing FengGang WeiLinfei ZhangHuadong ZhouWei WangPeng GuoCaitao ChengLei JiQinghe CaiYong FengHuahua TuTaylor & Francis Grouparticlehepatocellular carcinomadars-as1ckap2fak-erk pathwaymir-3200-5pmetastasisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8217-8232 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatocellular carcinoma dars-as1 ckap2 fak-erk pathway mir-3200-5p metastasis Biotechnology TP248.13-248.65
spellingShingle	hepatocellular carcinoma dars-as1 ckap2 fak-erk pathway mir-3200-5p metastasis Biotechnology TP248.13-248.65 Yanqing Feng Gang Wei Linfei Zhang Huadong Zhou Wei Wang Peng Guo Caitao Cheng Lei Ji Qinghe Cai Yong Feng Huahua Tu LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
description	Accumulating signs have found that long noncoding RNAs (lncRNAs) contribute to hepatocellular carcinoma (HCC). Here, we probed the effect and mechanism of lncRNA DARS-AS1 in HCC. The profiles of DARS-AS1 and Cytoskeleton associated protein 2 (CKAP2) in 50 HCC tissues and non-tumor tissues were examined by real-time quantitative polymerase chain reaction (RT-qPCR). DARS-AS1 and CKAP2 overexpression and/or knockdown cell models were established. The proliferation, apoptosis, invasion and epithelial-mesenchymal transition (EMT) were determined. CKAP2, and focal adhesion kinase (FAK)-extracellular signal-regulated kinase (ERK) was tested by Western blot (WB). The relationship between DARS-AS1 and CKAP2 was predicted by Bioinformatics, and the dual-luciferase reporter assay was applied to verify the targeting association between miR-3200-5p and DARS-AS1 and CKAP2. DARS-AS1 was overexpressed in HCC tissues (vs. that in non-tumor tissues) and was closely correlated with the patients’ tumor stage. DARS-AS1 facilitated HCC cell proliferation and hampered apoptosis. HCC cell migration and EMT were enhanced by DARS-AS1. DARS-AS1 up-regulated CKAP2, which aggravated HCC. Further investigation illustrated that either DARS-AS1 or CKAP2 activated FAK-ERK pathway, and miR-3200-5p was competitively restrained by DARS-AS1. miR-3200-5p exerted tumor-suppressive effects in HCC and inactivated CKAP2 and FAK-ERK pathway. All in all, this study corroborates that DARS-AS1 facilitates HCC proliferation and metastasis by regulating miR-3200-5p-mediated CKAP2, which provides a potential target for HCC diagnosis and treatment. Abbreviations: CCK-8: cell counting kit-8; CKAP2: Cytoskeleton associated protein 2; cDNA:complementary DNA; DAPI: 4ʹ,6-diamidino-2-phenylindole; DARS-AS1: DARS1 antisense RNA 1; DEPC: diethyl pyrocarbonate; DMEM-F12: Dulbecco’s minimal essential medium/Ham’s-F12; EMT: epithelial-mesenchymal transition; ERK: extracellular signal-regulated kinase; FAK: focal adhesion kinase; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HCC: hepatocellular carcinoma; HE: hematoxylin-eosin; IHC: Immunohistochemistry; LIHC: Liver hepatocellular carcinoma; lncRNAs: long noncoding RNAs; MIAT: lncRNA myocardial infarction-related transcripts; MT: Mutant; NC: negative control; PBS: phosphate-buffered saline; PMSF: Phenylmethylsulfonyl fluoride; PVDF: polyvinylidene difluoride; RT: room temperature; RT-qPCR: real-time quantitative polymerase chain reaction; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SPF: specific pathogen-free; TMAP: tumor-associated microtubule-associated protein; TUNEL: TdT-mediated dUTP nick end labeling; V: volume; WT: wild type.
format	article
author	Yanqing Feng Gang Wei Linfei Zhang Huadong Zhou Wei Wang Peng Guo Caitao Cheng Lei Ji Qinghe Cai Yong Feng Huahua Tu
author_facet	Yanqing Feng Gang Wei Linfei Zhang Huadong Zhou Wei Wang Peng Guo Caitao Cheng Lei Ji Qinghe Cai Yong Feng Huahua Tu
author_sort	Yanqing Feng
title	LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
title_short	LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
title_full	LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
title_fullStr	LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
title_full_unstemmed	LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis
title_sort	lncrna dars-as1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the mir-3200-5p-cytoskeleton associated protein 2 (ckap2) axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d6cccd44fdbd499bb6c86084e32240d8
work_keys_str_mv	AT yanqingfeng lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT gangwei lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT linfeizhang lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT huadongzhou lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT weiwang lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT pengguo lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT caitaocheng lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT leiji lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT qinghecai lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT yongfeng lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis AT huahuatu lncrnadarsas1aggravatesthegrowthandmetastasisofhepatocellularcarcinomaviaregulatingthemir32005pcytoskeletonassociatedprotein2ckap2axis
_version_	1718444742782484480

LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

Ejemplares similares