The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor

Abstract To explore the pathogenesis of high myopia (HM) using quantitative proteomics. The aqueous humor of patients with simple nuclear cataract and nuclear cataract complicated with HM (hereinafter referred to as “C” and “HM” groups, respectively) were collected. The isobaric tags for relative an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kai Wen, Xianfeng Shao, Yahong Li, Yaoling Li, Yongtao Li, Qing Wang, Ruihong Su, Lujie Zhang, Yang Cai, Jing Sun, Yan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6cf4d3b9bd64aee8f440bad46c70dfc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d6cf4d3b9bd64aee8f440bad46c70dfc
record_format	dspace
spelling	oai:doaj.org-article:d6cf4d3b9bd64aee8f440bad46c70dfc2021-12-02T16:45:14ZThe plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor10.1038/s41598-021-88220-92045-2322https://doaj.org/article/d6cf4d3b9bd64aee8f440bad46c70dfc2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88220-9https://doaj.org/toc/2045-2322Abstract To explore the pathogenesis of high myopia (HM) using quantitative proteomics. The aqueous humor of patients with simple nuclear cataract and nuclear cataract complicated with HM (hereinafter referred to as “C” and “HM” groups, respectively) were collected. The isobaric tags for relative and absolute quantitation (iTRAQ)-based liquid chromatography–tandem mass spectrometry (LC–MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs). Bioinformatics was used to interpret the proteomic results. Furthermore, the plasminogen (PLG) protein was confirmed by enzyme-linked immunosorbent assay (ELISA) as the candidate biomarker for HM through a receiver operating characteristic curve analysis. The study showed 32 upregulated and 26 downregulated proteins. The gene ontology analysis demonstrated that 58 DEPs corresponded to 325 biological processes, 33 cell components, and 45 molecular functional annotations. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the upregulated DEPs were highly enriched in the coagulation and complement cascades, consistent with the gene set enrichment analysis. Our data suggested that some DEPs might be hallmarks of the development of HM. ELISA confirmed that the PLG expression levels were significantly upregulated in HM. This was a new study investigating alterations in protein levels and affected pathways in HM using iTRAQ-based quantitative proteomics. Our study provided a comprehensive dataset on overall protein changes and shed light on its potential molecular mechanism in human HM.Kai WenXianfeng ShaoYahong LiYaoling LiYongtao LiQing WangRuihong SuLujie ZhangYang CaiJing SunYan ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Wen Xianfeng Shao Yahong Li Yaoling Li Yongtao Li Qing Wang Ruihong Su Lujie Zhang Yang Cai Jing Sun Yan Zhang The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
description	Abstract To explore the pathogenesis of high myopia (HM) using quantitative proteomics. The aqueous humor of patients with simple nuclear cataract and nuclear cataract complicated with HM (hereinafter referred to as “C” and “HM” groups, respectively) were collected. The isobaric tags for relative and absolute quantitation (iTRAQ)-based liquid chromatography–tandem mass spectrometry (LC–MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs). Bioinformatics was used to interpret the proteomic results. Furthermore, the plasminogen (PLG) protein was confirmed by enzyme-linked immunosorbent assay (ELISA) as the candidate biomarker for HM through a receiver operating characteristic curve analysis. The study showed 32 upregulated and 26 downregulated proteins. The gene ontology analysis demonstrated that 58 DEPs corresponded to 325 biological processes, 33 cell components, and 45 molecular functional annotations. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the upregulated DEPs were highly enriched in the coagulation and complement cascades, consistent with the gene set enrichment analysis. Our data suggested that some DEPs might be hallmarks of the development of HM. ELISA confirmed that the PLG expression levels were significantly upregulated in HM. This was a new study investigating alterations in protein levels and affected pathways in HM using iTRAQ-based quantitative proteomics. Our study provided a comprehensive dataset on overall protein changes and shed light on its potential molecular mechanism in human HM.
format	article
author	Kai Wen Xianfeng Shao Yahong Li Yaoling Li Yongtao Li Qing Wang Ruihong Su Lujie Zhang Yang Cai Jing Sun Yan Zhang
author_facet	Kai Wen Xianfeng Shao Yahong Li Yaoling Li Yongtao Li Qing Wang Ruihong Su Lujie Zhang Yang Cai Jing Sun Yan Zhang
author_sort	Kai Wen
title	The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
title_short	The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
title_full	The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
title_fullStr	The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
title_full_unstemmed	The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor
title_sort	plasminogen protein is associated with high myopia as revealed by the itraq-based proteomic analysis of the aqueous humor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d6cf4d3b9bd64aee8f440bad46c70dfc
work_keys_str_mv	AT kaiwen theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT xianfengshao theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yahongli theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yaolingli theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yongtaoli theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT qingwang theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT ruihongsu theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT lujiezhang theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yangcai theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT jingsun theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yanzhang theplasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT kaiwen plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT xianfengshao plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yahongli plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yaolingli plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yongtaoli plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT qingwang plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT ruihongsu plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT lujiezhang plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yangcai plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT jingsun plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor AT yanzhang plasminogenproteinisassociatedwithhighmyopiaasrevealedbytheitraqbasedproteomicanalysisoftheaqueoushumor
_version_	1718383484077080576

The plasminogen protein is associated with high myopia as revealed by the iTRAQ-based proteomic analysis of the aqueous humor

Ejemplares similares