Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.

It is well known that exosomes could serve as anti-microbial immune factors in animals. However, despite growing evidences have shown that the homeostasis of the hemolymph microbiota was vital for immune regulation in crustaceans, the relationship between exosomes and hemolymph microbiota homeostasi...

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Autores principales:	Yi Gong, Xiaoyuan Wei, Wanwei Sun, Xin Ren, Jiao Chen, Jude Juventus Aweya, Hongyu Ma, Kok-Gan Chan, Yueling Zhang, Shengkang Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d6d5388b7c24422aaa85b398d0157f92
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spelling	oai:doaj.org-article:d6d5388b7c24422aaa85b398d0157f922021-12-02T20:00:17ZExosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.1553-73661553-737410.1371/journal.ppat.1009837https://doaj.org/article/d6d5388b7c24422aaa85b398d0157f922021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009837https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374It is well known that exosomes could serve as anti-microbial immune factors in animals. However, despite growing evidences have shown that the homeostasis of the hemolymph microbiota was vital for immune regulation in crustaceans, the relationship between exosomes and hemolymph microbiota homeostasis during pathogenic bacteria infection has not been addressed. Here, we reported that exosomes released from Vibrio parahaemolyticus-infected mud crabs (Scylla paramamosain) could help to maintain the homeostasis of hemolymph microbiota and have a protective effect on the mortality of the host during the infection process. We further confirmed that miR-224 was densely packaged in these exosomes, resulting in the suppression of HSP70 and disruption of the HSP70-TRAF6 complex, then the released TRAF6 further interacted with Ecsit to regulate the production of mitochondrial ROS (mROS) and the expression of Anti-lipopolysaccharide factors (ALFs) in recipient hemocytes, which eventually affected hemolymph microbiota homeostasis in response to the pathogenic bacteria infection in mud crab. To the best of our knowledge, this is the first document that reports the role of exosome in the hemolymph microbiota homeostasis modulation during pathogen infection, which reveals the crosstalk between exosomal miRNAs and innate immune response in crustaceans.Yi GongXiaoyuan WeiWanwei SunXin RenJiao ChenJude Juventus AweyaHongyu MaKok-Gan ChanYueling ZhangShengkang LiPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009837 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Yi Gong Xiaoyuan Wei Wanwei Sun Xin Ren Jiao Chen Jude Juventus Aweya Hongyu Ma Kok-Gan Chan Yueling Zhang Shengkang Li Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
description	It is well known that exosomes could serve as anti-microbial immune factors in animals. However, despite growing evidences have shown that the homeostasis of the hemolymph microbiota was vital for immune regulation in crustaceans, the relationship between exosomes and hemolymph microbiota homeostasis during pathogenic bacteria infection has not been addressed. Here, we reported that exosomes released from Vibrio parahaemolyticus-infected mud crabs (Scylla paramamosain) could help to maintain the homeostasis of hemolymph microbiota and have a protective effect on the mortality of the host during the infection process. We further confirmed that miR-224 was densely packaged in these exosomes, resulting in the suppression of HSP70 and disruption of the HSP70-TRAF6 complex, then the released TRAF6 further interacted with Ecsit to regulate the production of mitochondrial ROS (mROS) and the expression of Anti-lipopolysaccharide factors (ALFs) in recipient hemocytes, which eventually affected hemolymph microbiota homeostasis in response to the pathogenic bacteria infection in mud crab. To the best of our knowledge, this is the first document that reports the role of exosome in the hemolymph microbiota homeostasis modulation during pathogen infection, which reveals the crosstalk between exosomal miRNAs and innate immune response in crustaceans.
format	article
author	Yi Gong Xiaoyuan Wei Wanwei Sun Xin Ren Jiao Chen Jude Juventus Aweya Hongyu Ma Kok-Gan Chan Yueling Zhang Shengkang Li
author_facet	Yi Gong Xiaoyuan Wei Wanwei Sun Xin Ren Jiao Chen Jude Juventus Aweya Hongyu Ma Kok-Gan Chan Yueling Zhang Shengkang Li
author_sort	Yi Gong
title	Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
title_short	Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
title_full	Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
title_fullStr	Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
title_full_unstemmed	Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
title_sort	exosomal mir-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d6d5388b7c24422aaa85b398d0157f92
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Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.

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