Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation

Abstract Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the magnetic levitation system to facilitate biofabrication of in situ three...

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Autores principales:	Muge Anil-Inevi, Sena Yaman, Ahu Arslan Yildiz, Gulistan Mese, Ozden Yalcin-Ozuysal, H. Cumhur Tekin, Engin Ozcivici
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6d5a2a570c942d7b88bd9e6f2c86200
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spelling	oai:doaj.org-article:d6d5a2a570c942d7b88bd9e6f2c862002021-12-02T15:09:10ZBiofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation10.1038/s41598-018-25718-92045-2322https://doaj.org/article/d6d5a2a570c942d7b88bd9e6f2c862002018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25718-9https://doaj.org/toc/2045-2322Abstract Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the magnetic levitation system to facilitate biofabrication of in situ three dimensional (3D) cellular structures. Here, we optimized a magnetic levitation though negative magnetophoresis protocol appropriate for long term levitated cell culture and developed an in situ 3D cellular assembly model with controlled cluster size and cellular pattern under simulated weightlessness. The developed strategy outlines a potential basis for the study of weightlessness on 3D living structures and with the opportunity for real-time imaging that is not possible with current ground-based simulated weightlessness techniques. The low-cost technique presented here may offer a wide range of biomedical applications in several research fields, including mechanobiology, drug discovery and developmental biology.Muge Anil-IneviSena YamanAhu Arslan YildizGulistan MeseOzden Yalcin-OzuysalH. Cumhur TekinEngin OzciviciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muge Anil-Inevi Sena Yaman Ahu Arslan Yildiz Gulistan Mese Ozden Yalcin-Ozuysal H. Cumhur Tekin Engin Ozcivici Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
description	Abstract Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the magnetic levitation system to facilitate biofabrication of in situ three dimensional (3D) cellular structures. Here, we optimized a magnetic levitation though negative magnetophoresis protocol appropriate for long term levitated cell culture and developed an in situ 3D cellular assembly model with controlled cluster size and cellular pattern under simulated weightlessness. The developed strategy outlines a potential basis for the study of weightlessness on 3D living structures and with the opportunity for real-time imaging that is not possible with current ground-based simulated weightlessness techniques. The low-cost technique presented here may offer a wide range of biomedical applications in several research fields, including mechanobiology, drug discovery and developmental biology.
format	article
author	Muge Anil-Inevi Sena Yaman Ahu Arslan Yildiz Gulistan Mese Ozden Yalcin-Ozuysal H. Cumhur Tekin Engin Ozcivici
author_facet	Muge Anil-Inevi Sena Yaman Ahu Arslan Yildiz Gulistan Mese Ozden Yalcin-Ozuysal H. Cumhur Tekin Engin Ozcivici
author_sort	Muge Anil-Inevi
title	Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
title_short	Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
title_full	Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
title_fullStr	Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
title_full_unstemmed	Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation
title_sort	biofabrication of in situ self assembled 3d cell cultures in a weightlessness environment generated using magnetic levitation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d6d5a2a570c942d7b88bd9e6f2c86200
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Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation

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