Comparison of three qPCR-based commercial tests for detection of periodontal pathogens

Abstract In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three comm...

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Autores principales:	Fridus Van der Weijden, Mirella Rijnen, Cees Valkenburg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6ddd2dd56ec4264a7a883ebe7bd54d7
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spelling	oai:doaj.org-article:d6ddd2dd56ec4264a7a883ebe7bd54d72021-12-02T11:39:27ZComparison of three qPCR-based commercial tests for detection of periodontal pathogens10.1038/s41598-021-85305-32045-2322https://doaj.org/article/d6ddd2dd56ec4264a7a883ebe7bd54d72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85305-3https://doaj.org/toc/2045-2322Abstract In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three commercially available qPCR kits for detection and quantification of selected periodontal bacterial species were compared, using 100 clinical samples from patients with untreated periodontitis. The analysis involved two separate comparisons in which kit A (LabOral Diagnostics, The Netherlands) was compared with kit B (Advanced Dental Diagnostics, The Netherlands), and with kit C (OralDent diagnostics, The Netherlands). Analytic procedures for detection and quantification of selected periodontal bacterial species were carried out according to the instructions of the laboratories. Kit A detected target species more often, and absolute numbers of bacterial cells were higher than with kit B. A high degree of similarity was found between the test outcomes by kit A and kit C. All three kits performed satisfactory but small and significant differences exist between kits.Fridus Van der WeijdenMirella RijnenCees ValkenburgNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fridus Van der Weijden Mirella Rijnen Cees Valkenburg Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
description	Abstract In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three commercially available qPCR kits for detection and quantification of selected periodontal bacterial species were compared, using 100 clinical samples from patients with untreated periodontitis. The analysis involved two separate comparisons in which kit A (LabOral Diagnostics, The Netherlands) was compared with kit B (Advanced Dental Diagnostics, The Netherlands), and with kit C (OralDent diagnostics, The Netherlands). Analytic procedures for detection and quantification of selected periodontal bacterial species were carried out according to the instructions of the laboratories. Kit A detected target species more often, and absolute numbers of bacterial cells were higher than with kit B. A high degree of similarity was found between the test outcomes by kit A and kit C. All three kits performed satisfactory but small and significant differences exist between kits.
format	article
author	Fridus Van der Weijden Mirella Rijnen Cees Valkenburg
author_facet	Fridus Van der Weijden Mirella Rijnen Cees Valkenburg
author_sort	Fridus Van der Weijden
title	Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
title_short	Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
title_full	Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
title_fullStr	Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
title_full_unstemmed	Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
title_sort	comparison of three qpcr-based commercial tests for detection of periodontal pathogens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d6ddd2dd56ec4264a7a883ebe7bd54d7
work_keys_str_mv	AT fridusvanderweijden comparisonofthreeqpcrbasedcommercialtestsfordetectionofperiodontalpathogens AT mirellarijnen comparisonofthreeqpcrbasedcommercialtestsfordetectionofperiodontalpathogens AT ceesvalkenburg comparisonofthreeqpcrbasedcommercialtestsfordetectionofperiodontalpathogens
_version_	1718395731381846016

Comparison of three qPCR-based commercial tests for detection of periodontal pathogens

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