An investigation of fecal volatile organic metabolites in irritable bowel syndrome.

Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid o...

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Autores principales:	Iftikhar Ahmed, Rosemary Greenwood, Ben de Lacy Costello, Norman M Ratcliffe, Chris S Probert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6e2474676cd4438a4e43397e09a30ad
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spelling	oai:doaj.org-article:d6e2474676cd4438a4e43397e09a30ad2021-11-18T07:53:41ZAn investigation of fecal volatile organic metabolites in irritable bowel syndrome.1932-620310.1371/journal.pone.0058204https://doaj.org/article/d6e2474676cd4438a4e43397e09a30ad2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516449/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p<0.05), while aldehydes were more abundant in IBD (p<0.05). A predictive model, developed by multivariate analysis, separated IBS-D from active CD, UC and healthy controls with a sensitivity of 94%, 96% and 90%; and a specificity of 82%, 80% and 80% respectively (p<0.05). The understanding of the derivation of these VOMs may cast light on the etiology of IBS-D and IBD. These data show that fecal VOMs analyses could contribute to the diagnosis of IBS-D, for which there is no laboratory test, as well as IBD.Iftikhar AhmedRosemary GreenwoodBen de Lacy CostelloNorman M RatcliffeChris S ProbertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58204 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iftikhar Ahmed Rosemary Greenwood Ben de Lacy Costello Norman M Ratcliffe Chris S Probert An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
description	Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p<0.05), while aldehydes were more abundant in IBD (p<0.05). A predictive model, developed by multivariate analysis, separated IBS-D from active CD, UC and healthy controls with a sensitivity of 94%, 96% and 90%; and a specificity of 82%, 80% and 80% respectively (p<0.05). The understanding of the derivation of these VOMs may cast light on the etiology of IBS-D and IBD. These data show that fecal VOMs analyses could contribute to the diagnosis of IBS-D, for which there is no laboratory test, as well as IBD.
format	article
author	Iftikhar Ahmed Rosemary Greenwood Ben de Lacy Costello Norman M Ratcliffe Chris S Probert
author_facet	Iftikhar Ahmed Rosemary Greenwood Ben de Lacy Costello Norman M Ratcliffe Chris S Probert
author_sort	Iftikhar Ahmed
title	An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
title_short	An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
title_full	An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
title_fullStr	An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
title_full_unstemmed	An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
title_sort	investigation of fecal volatile organic metabolites in irritable bowel syndrome.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d6e2474676cd4438a4e43397e09a30ad
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An investigation of fecal volatile organic metabolites in irritable bowel syndrome.

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