A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds

Abstract We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation...

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Autores principales:	Frederic Strobl, Ernst H. K. Stelzer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d6e852fb38c74d2181d8df322ae11138
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spelling	oai:doaj.org-article:d6e852fb38c74d2181d8df322ae111382021-12-02T16:23:43ZA deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds10.1038/s41598-021-94288-02045-2322https://doaj.org/article/d6e852fb38c74d2181d8df322ae111382021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94288-0https://doaj.org/toc/2045-2322Abstract We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.Frederic StroblErnst H. K. StelzerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frederic Strobl Ernst H. K. Stelzer A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
description	Abstract We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
format	article
author	Frederic Strobl Ernst H. K. Stelzer
author_facet	Frederic Strobl Ernst H. K. Stelzer
author_sort	Frederic Strobl
title	A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_short	A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_full	A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_fullStr	A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_full_unstemmed	A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_sort	deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d6e852fb38c74d2181d8df322ae11138
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A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds

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