Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.

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Bibliographic Details
Main Authors:	Yingxiao Zhang, Qiurong Ren, Xu Tang, Shishi Liu, Aimee A. Malzahn, Jianping Zhou, Jiaheng Wang, Desuo Yin, Changtian Pan, Mingzhu Yuan, Lan Huang, Han Yang, Yuxin Zhao, Qing Fang, Xuelian Zheng, Li Tian, Yanhao Cheng, Ysa Le, Bailey McCoy, Lidiya Franklin, Jeremy D. Selengut, Stephen M. Mount, Qiudeng Que, Yong Zhang, Yiping Qi
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/d6eb08b3515f47118d396c6a80d7329c
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spelling	oai:doaj.org-article:d6eb08b3515f47118d396c6a80d7329c2021-12-02T14:25:31ZExpanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems10.1038/s41467-021-22330-w2041-1723https://doaj.org/article/d6eb08b3515f47118d396c6a80d7329c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22330-whttps://doaj.org/toc/2041-1723CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.Yingxiao ZhangQiurong RenXu TangShishi LiuAimee A. MalzahnJianping ZhouJiaheng WangDesuo YinChangtian PanMingzhu YuanLan HuangHan YangYuxin ZhaoQing FangXuelian ZhengLi TianYanhao ChengYsa LeBailey McCoyLidiya FranklinJeremy D. SelengutStephen M. MountQiudeng QueYong ZhangYiping QiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yingxiao Zhang Qiurong Ren Xu Tang Shishi Liu Aimee A. Malzahn Jianping Zhou Jiaheng Wang Desuo Yin Changtian Pan Mingzhu Yuan Lan Huang Han Yang Yuxin Zhao Qing Fang Xuelian Zheng Li Tian Yanhao Cheng Ysa Le Bailey McCoy Lidiya Franklin Jeremy D. Selengut Stephen M. Mount Qiudeng Que Yong Zhang Yiping Qi Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
description	CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.
format	article
author	Yingxiao Zhang Qiurong Ren Xu Tang Shishi Liu Aimee A. Malzahn Jianping Zhou Jiaheng Wang Desuo Yin Changtian Pan Mingzhu Yuan Lan Huang Han Yang Yuxin Zhao Qing Fang Xuelian Zheng Li Tian Yanhao Cheng Ysa Le Bailey McCoy Lidiya Franklin Jeremy D. Selengut Stephen M. Mount Qiudeng Que Yong Zhang Yiping Qi
author_facet	Yingxiao Zhang Qiurong Ren Xu Tang Shishi Liu Aimee A. Malzahn Jianping Zhou Jiaheng Wang Desuo Yin Changtian Pan Mingzhu Yuan Lan Huang Han Yang Yuxin Zhao Qing Fang Xuelian Zheng Li Tian Yanhao Cheng Ysa Le Bailey McCoy Lidiya Franklin Jeremy D. Selengut Stephen M. Mount Qiudeng Que Yong Zhang Yiping Qi
author_sort	Yingxiao Zhang
title	Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
title_short	Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
title_full	Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
title_fullStr	Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
title_full_unstemmed	Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
title_sort	expanding the scope of plant genome engineering with cas12a orthologs and highly multiplexable editing systems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d6eb08b3515f47118d396c6a80d7329c
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Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

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