Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.

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Autores principales: Yingxiao Zhang, Qiurong Ren, Xu Tang, Shishi Liu, Aimee A. Malzahn, Jianping Zhou, Jiaheng Wang, Desuo Yin, Changtian Pan, Mingzhu Yuan, Lan Huang, Han Yang, Yuxin Zhao, Qing Fang, Xuelian Zheng, Li Tian, Yanhao Cheng, Ysa Le, Bailey McCoy, Lidiya Franklin, Jeremy D. Selengut, Stephen M. Mount, Qiudeng Que, Yong Zhang, Yiping Qi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/d6eb08b3515f47118d396c6a80d7329c
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