EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS

BACKGROUND AND OBJECTIVE: According to the increasing resistance of Candida species to anticandidal drugs in some patients, several researches were led to find out new antifungal agents from natural sources. The aim of this study was to evaluate the anticandidal activity of honey experimentally.METH...

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Autores principales:	S Mahdavi Omran, GH Maliji, AA Sefidgar, MR Yosefi, M Haji Ahmadi, SJ Moosavi, M Shafii
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2008
Materias:	honey candida minimum inhibitory concentration (mic) minimum fungicidal concentration (mfc) Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d6fe34238eaa46339f20e8792115cb26
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spelling	oai:doaj.org-article:d6fe34238eaa46339f20e8792115cb262021-11-10T09:07:50ZEFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS1561-41072251-7170https://doaj.org/article/d6fe34238eaa46339f20e8792115cb262008-12-01T00:00:00Zhttp://jbums.org/article-1-3096-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: According to the increasing resistance of Candida species to anticandidal drugs in some patients, several researches were led to find out new antifungal agents from natural sources. The aim of this study was to evaluate the anticandidal activity of honey experimentally.METHODS: In the present study, physical, biochemical and microbial characterization of four types of honey from North of Iran were determined. Different dilution (15-75%) of honey, amphotericin B (0.008-16µg/ml) and fluconazole (0.06-128µg/ml) were used on two species of candida albicans and one species of candida dubliniensis. Micro plates were incubated at 25 °C and 37°C for 48 hours with shaking (150 rpm). 10µl of transparent wells were plated onto sabouraud dextrose agar plates and calculated minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The anti-candida activity of honey and drugs were analyzed by using t-Student, Mann-Whitney and ANOVA tests.FINDINGS: It was not grown any organisms on honey cultures. Different dilution of honey in 20-25% and 15-30% can inhibit candidal growth at 25 °C and 37 °C (MIC50). MFC for honey at 25 °C and 37 °C were 40-70% and 45-70%, respectively. The differences between anticandidal effects of honey were not statistically significant. Candidal growth was inhibited (50%) by amphotericin B in 0.03-0.125µg/ml and 0.03-0.06µg/ml at 25 °C and 37 °C, respectively. MFC for this drug and temperature above mentioned were 0.5 and 0.125 µg/mg, respectively. MIC for fluconazole at 25 °C and 37 °C were 2-4 µg/ml and 0.25-0.5 µg/ml, respectively fluconazole inhibited candida growth, totally (MFC) at 25 °C and 37 °C in 32-64 µg/ml and 16-32µg/ml, respectively.CONCLUSION: With regard to the anticandidal effects on honey, specially unheated old honey on candida dubliniensis at 37 °C, so honey could be considered as antifungal component in the treatment of experimental infections.S Mahdavi Omran,GH MalijiAA Sefidgar,MR YosefiM Haji AhmadiSJ Moosavi,M ShafiiBabol University of Medical Sciencesarticlehoneycandidaminimum inhibitory concentration (mic)minimum fungicidal concentration (mfc)MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 10, Iss 5, Pp 15-22 (2008)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	honey candida minimum inhibitory concentration (mic) minimum fungicidal concentration (mfc) Medicine R Medicine (General) R5-920
spellingShingle	honey candida minimum inhibitory concentration (mic) minimum fungicidal concentration (mfc) Medicine R Medicine (General) R5-920 S Mahdavi Omran, GH Maliji AA Sefidgar, MR Yosefi M Haji Ahmadi SJ Moosavi, M Shafii EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
description	BACKGROUND AND OBJECTIVE: According to the increasing resistance of Candida species to anticandidal drugs in some patients, several researches were led to find out new antifungal agents from natural sources. The aim of this study was to evaluate the anticandidal activity of honey experimentally.METHODS: In the present study, physical, biochemical and microbial characterization of four types of honey from North of Iran were determined. Different dilution (15-75%) of honey, amphotericin B (0.008-16µg/ml) and fluconazole (0.06-128µg/ml) were used on two species of candida albicans and one species of candida dubliniensis. Micro plates were incubated at 25 °C and 37°C for 48 hours with shaking (150 rpm). 10µl of transparent wells were plated onto sabouraud dextrose agar plates and calculated minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The anti-candida activity of honey and drugs were analyzed by using t-Student, Mann-Whitney and ANOVA tests.FINDINGS: It was not grown any organisms on honey cultures. Different dilution of honey in 20-25% and 15-30% can inhibit candidal growth at 25 °C and 37 °C (MIC50). MFC for honey at 25 °C and 37 °C were 40-70% and 45-70%, respectively. The differences between anticandidal effects of honey were not statistically significant. Candidal growth was inhibited (50%) by amphotericin B in 0.03-0.125µg/ml and 0.03-0.06µg/ml at 25 °C and 37 °C, respectively. MFC for this drug and temperature above mentioned were 0.5 and 0.125 µg/mg, respectively. MIC for fluconazole at 25 °C and 37 °C were 2-4 µg/ml and 0.25-0.5 µg/ml, respectively fluconazole inhibited candida growth, totally (MFC) at 25 °C and 37 °C in 32-64 µg/ml and 16-32µg/ml, respectively.CONCLUSION: With regard to the anticandidal effects on honey, specially unheated old honey on candida dubliniensis at 37 °C, so honey could be considered as antifungal component in the treatment of experimental infections.
format	article
author	S Mahdavi Omran, GH Maliji AA Sefidgar, MR Yosefi M Haji Ahmadi SJ Moosavi, M Shafii
author_facet	S Mahdavi Omran, GH Maliji AA Sefidgar, MR Yosefi M Haji Ahmadi SJ Moosavi, M Shafii
author_sort	S Mahdavi Omran,
title	EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
title_short	EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
title_full	EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
title_fullStr	EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
title_full_unstemmed	EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS
title_sort	effect of honey from north of iran on candida albicans
publisher	Babol University of Medical Sciences
publishDate	2008
url	https://doaj.org/article/d6fe34238eaa46339f20e8792115cb26
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EFFECT OF HONEY FROM NORTH OF IRAN ON CANDIDA ALBICANS

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