Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation

Liew et al. describe an efficient monoclonal antibody (mAb) production method and as a proof-of-concept, develop mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for...

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Autores principales:	Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d6fe975bf6344cbab8916f95a52e69a6
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spelling	oai:doaj.org-article:d6fe975bf6344cbab8916f95a52e69a62021-12-02T14:37:38ZEpitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation10.1038/s42003-021-01965-x2399-3642https://doaj.org/article/d6fe975bf6344cbab8916f95a52e69a62021-04-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01965-xhttps://doaj.org/toc/2399-3642Liew et al. describe an efficient monoclonal antibody (mAb) production method and as a proof-of-concept, develop mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.Oi Wah LiewSamantha S. M. LingShera LilyannaYue ZhouPeipei WangJenny P. C. ChongYan Xia NgAngeline E. S. LimEliot R. Y. LeongQifeng LinTeck Kwang LimQinsong LinEnoch M. W. NgTuck Wah NgA. Mark RichardsNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Oi Wah Liew Samantha S. M. Ling Shera Lilyanna Yue Zhou Peipei Wang Jenny P. C. Chong Yan Xia Ng Angeline E. S. Lim Eliot R. Y. Leong Qifeng Lin Teck Kwang Lim Qinsong Lin Enoch M. W. Ng Tuck Wah Ng A. Mark Richards Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
description	Liew et al. describe an efficient monoclonal antibody (mAb) production method and as a proof-of-concept, develop mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
format	article
author	Oi Wah Liew Samantha S. M. Ling Shera Lilyanna Yue Zhou Peipei Wang Jenny P. C. Chong Yan Xia Ng Angeline E. S. Lim Eliot R. Y. Leong Qifeng Lin Teck Kwang Lim Qinsong Lin Enoch M. W. Ng Tuck Wah Ng A. Mark Richards
author_facet	Oi Wah Liew Samantha S. M. Ling Shera Lilyanna Yue Zhou Peipei Wang Jenny P. C. Chong Yan Xia Ng Angeline E. S. Lim Eliot R. Y. Leong Qifeng Lin Teck Kwang Lim Qinsong Lin Enoch M. W. Ng Tuck Wah Ng A. Mark Richards
author_sort	Oi Wah Liew
title	Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
title_short	Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
title_full	Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
title_fullStr	Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
title_full_unstemmed	Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
title_sort	epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d6fe975bf6344cbab8916f95a52e69a6
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Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation

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