Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy

Pinus Koraiensis seeds have physiological dormancy. Cold stratification releases seed dormancy. The changes in metabolite profiles of dormant seeds and cold stratified seeds during shorter incubation time in a favorable condition for seed germination have been studied. However, a more-long-term dete...

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Autores principales:	Yuan Song, Xiaoye Gao, Yunjie Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Korean pine dormancy germination metabolomics embryo megagametophyte Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d6ffc4e6519141348e787209aefb2dfc
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spelling	oai:doaj.org-article:d6ffc4e6519141348e787209aefb2dfc2021-11-30T10:10:11ZKey Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy1664-462X10.3389/fpls.2021.767108https://doaj.org/article/d6ffc4e6519141348e787209aefb2dfc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.767108/fullhttps://doaj.org/toc/1664-462XPinus Koraiensis seeds have physiological dormancy. Cold stratification releases seed dormancy. The changes in metabolite profiles of dormant seeds and cold stratified seeds during shorter incubation time in a favorable condition for seed germination have been studied. However, a more-long-term detection of the changes in metabolites in dormant seeds can identify the real metabolic pathways responsible for dormancy. Metabolite composition was investigated in embryo and megagametophyte of primary physiological dormant seeds (DS) of P. Koraiensis collected at 0, 1, 2, 4, and 6 weeks of incubation and of non-primary physiological dormant seeds (NDS) sampled at 0 and 1 week of incubation, seed coat rupture stage, and radicle protrusion stage. Embryos contained higher levels of most metabolites than megagametophyte. Strong accumulation of most metabolites in DS occurred at 1 and 4 weeks of incubation. A larger reduction in the relative levels of most phosphorylated sugars and amino acids in NDS was found between 1-week-incubation and seed coat rupture stage. The relative levels of metabolites involved in carbohydrate metabolism, especially the pentose phosphate pathway (PPP) and tricarboxylic acid (TCA) cycle, were higher in the embryos of 4-week-incubated DS, but the relative contents of intermediate metabolites of most amino acid metabolism were lower compared to 1-week-incubated NDS. We suggested that the disturbed carbohydrate metabolism and amino acid metabolism in the embryos of DS after 4 weeks of incubation maybe related to primary dormancy. Our study provides information for a better understanding of the mechanism of seed dormancy.Yuan SongYuan SongXiaoye GaoYunjie WuFrontiers Media S.A.articleKorean pinedormancygerminationmetabolomicsembryomegagametophytePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Korean pine dormancy germination metabolomics embryo megagametophyte Plant culture SB1-1110
spellingShingle	Korean pine dormancy germination metabolomics embryo megagametophyte Plant culture SB1-1110 Yuan Song Yuan Song Xiaoye Gao Yunjie Wu Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
description	Pinus Koraiensis seeds have physiological dormancy. Cold stratification releases seed dormancy. The changes in metabolite profiles of dormant seeds and cold stratified seeds during shorter incubation time in a favorable condition for seed germination have been studied. However, a more-long-term detection of the changes in metabolites in dormant seeds can identify the real metabolic pathways responsible for dormancy. Metabolite composition was investigated in embryo and megagametophyte of primary physiological dormant seeds (DS) of P. Koraiensis collected at 0, 1, 2, 4, and 6 weeks of incubation and of non-primary physiological dormant seeds (NDS) sampled at 0 and 1 week of incubation, seed coat rupture stage, and radicle protrusion stage. Embryos contained higher levels of most metabolites than megagametophyte. Strong accumulation of most metabolites in DS occurred at 1 and 4 weeks of incubation. A larger reduction in the relative levels of most phosphorylated sugars and amino acids in NDS was found between 1-week-incubation and seed coat rupture stage. The relative levels of metabolites involved in carbohydrate metabolism, especially the pentose phosphate pathway (PPP) and tricarboxylic acid (TCA) cycle, were higher in the embryos of 4-week-incubated DS, but the relative contents of intermediate metabolites of most amino acid metabolism were lower compared to 1-week-incubated NDS. We suggested that the disturbed carbohydrate metabolism and amino acid metabolism in the embryos of DS after 4 weeks of incubation maybe related to primary dormancy. Our study provides information for a better understanding of the mechanism of seed dormancy.
format	article
author	Yuan Song Yuan Song Xiaoye Gao Yunjie Wu
author_facet	Yuan Song Yuan Song Xiaoye Gao Yunjie Wu
author_sort	Yuan Song
title	Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
title_short	Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
title_full	Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
title_fullStr	Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
title_full_unstemmed	Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy
title_sort	key metabolite differences between korean pine (pinus koraiensis) seeds with primary physiological dormancy and no-dormancy
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d6ffc4e6519141348e787209aefb2dfc
work_keys_str_mv	AT yuansong keymetabolitedifferencesbetweenkoreanpinepinuskoraiensisseedswithprimaryphysiologicaldormancyandnodormancy AT yuansong keymetabolitedifferencesbetweenkoreanpinepinuskoraiensisseedswithprimaryphysiologicaldormancyandnodormancy AT xiaoyegao keymetabolitedifferencesbetweenkoreanpinepinuskoraiensisseedswithprimaryphysiologicaldormancyandnodormancy AT yunjiewu keymetabolitedifferencesbetweenkoreanpinepinuskoraiensisseedswithprimaryphysiologicaldormancyandnodormancy
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Key Metabolite Differences Between Korean Pine (Pinus koraiensis) Seeds With Primary Physiological Dormancy and No-Dormancy

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