DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE

Forensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases o...

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Autores principales:	A. M. Lazitskaya, N. V. Chmelevskaya, E. A. Illarionova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	chemical and toxicological analysis isolation tofisopam Science Q
Acceso en línea:	https://doaj.org/article/d7012c13891d44c49079b742a1b0f797
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spelling	oai:doaj.org-article:d7012c13891d44c49079b742a1b0f7972021-11-23T06:14:37ZDEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE2541-94202587-959610.12737/article_5955e6b4db2812.30407534https://doaj.org/article/d7012c13891d44c49079b742a1b0f7972017-02-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/362https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Forensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases of tofisopam poisoning. And there are no descriptions of tofisopam isolation from biological fluids in the literature. Liquid extraction, an effective and commonly used method of isolation in the chemical-toxicological analysis, is used when extracting substances from blood, lymph, saliva, urine and stomach washings. The process of isolation may be influenced by the nature of the organic solvent, pH, the presence of an electrolyte, time and frequency of extraction. We have studied the effect of each factor on tofisopam isolation from aqueous solutions. It has been found that the optimal organic solvent for the extraction of the solutions is dichloroethane, which extracts maximum amount of the analyte at pH = 5.0. Saturated sodium sulphate has a salting-in effect for tofisopam. The optimum conditions for tofisopam isolating are 7 minutes with double extraction. Based on the research method of tofisopam isolating the model mixture of urine was developed. We found that this model urine mixture isolates 66.51-67.9l % of tofisopam. We performed validation assessment of the developed method for evaluation of precision and accuracy. The assessment showed the suitability of techniques for the analysis.A. M. LazitskayaN. V. ChmelevskayaE. A. IllarionovaScientific Сentre for Family Health and Human Reproduction Problemsarticlechemical and toxicological analysisisolationtofisopamScienceQRUActa Biomedica Scientifica, Vol 2, Iss 1, Pp 52-55 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	chemical and toxicological analysis isolation tofisopam Science Q
spellingShingle	chemical and toxicological analysis isolation tofisopam Science Q A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
description	Forensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases of tofisopam poisoning. And there are no descriptions of tofisopam isolation from biological fluids in the literature. Liquid extraction, an effective and commonly used method of isolation in the chemical-toxicological analysis, is used when extracting substances from blood, lymph, saliva, urine and stomach washings. The process of isolation may be influenced by the nature of the organic solvent, pH, the presence of an electrolyte, time and frequency of extraction. We have studied the effect of each factor on tofisopam isolation from aqueous solutions. It has been found that the optimal organic solvent for the extraction of the solutions is dichloroethane, which extracts maximum amount of the analyte at pH = 5.0. Saturated sodium sulphate has a salting-in effect for tofisopam. The optimum conditions for tofisopam isolating are 7 minutes with double extraction. Based on the research method of tofisopam isolating the model mixture of urine was developed. We found that this model urine mixture isolates 66.51-67.9l % of tofisopam. We performed validation assessment of the developed method for evaluation of precision and accuracy. The assessment showed the suitability of techniques for the analysis.
format	article
author	A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova
author_facet	A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova
author_sort	A. M. Lazitskaya
title	DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
title_short	DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
title_full	DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
title_fullStr	DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
title_full_unstemmed	DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
title_sort	development of methods of tofisopam isolation in model urine mixture
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/d7012c13891d44c49079b742a1b0f797
work_keys_str_mv	AT amlazitskaya developmentofmethodsoftofisopamisolationinmodelurinemixture AT nvchmelevskaya developmentofmethodsoftofisopamisolationinmodelurinemixture AT eaillarionova developmentofmethodsoftofisopamisolationinmodelurinemixture
_version_	1718417037234012160

DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE

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