Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies

Abstract Eosinophilic esophagitis (EoE) is a chronic, immune-mediated inflammatory esophageal disease triggered by food antigens. Cumulative evidence supports the implication of microbiota and the innate immune system in the pathogenesis of EoE. Changes in the esophageal microbiome were investigated...

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Autores principales:	E. J. Laserna-Mendieta, J. A. FitzGerald, L. Arias-Gonzalez, J. M. Ollala, D. Bernardo, M. J. Claesson, A. J. Lucendo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d705523ff13b43148c3bae3a919eeaee
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spelling	oai:doaj.org-article:d705523ff13b43148c3bae3a919eeaee2021-12-02T14:25:21ZEsophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies10.1038/s41598-021-86464-z2045-2322https://doaj.org/article/d705523ff13b43148c3bae3a919eeaee2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86464-zhttps://doaj.org/toc/2045-2322Abstract Eosinophilic esophagitis (EoE) is a chronic, immune-mediated inflammatory esophageal disease triggered by food antigens. Cumulative evidence supports the implication of microbiota and the innate immune system in the pathogenesis of EoE. Changes in the esophageal microbiome were investigated by applying 16S rRNA gene sequencing on esophageal biopsies of adult patients with active EoE at baseline (n = 30), and after achieving remission with either proton pump inhibitors (PPI, n = 10), swallowed topical corticosteroids (STC, n = 10) or food-elimination diets (FED, n = 10). Ten non-EoE biopsies were also characterized as controls. Compared to controls, no differences in alpha (intra-sample) diversity were found in EoE microbiota overall. However, it decreased significantly among patients who underwent FED. As for beta (inter-sample) diversity, non-EoE controls separated from EoE baseline samples. Post-treatment samples from patients treated with PPI and FED had a more similar microbiota composition, while those receiving STC were closer to controls. Differential testing of microbial relative abundance displayed significant changes for Filifactor, Parvimonas and Porphyromonas genera. Analysis of predicted functions indicated alterations in metabolic pathways and abundance of sulphur-cytochrome oxidoreductases. Our findings demonstrate changes in microbiota associated with EoE, as well as a treatment effect on the microbiome.E. J. Laserna-MendietaJ. A. FitzGeraldL. Arias-GonzalezJ. M. OllalaD. BernardoM. J. ClaessonA. J. LucendoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. J. Laserna-Mendieta J. A. FitzGerald L. Arias-Gonzalez J. M. Ollala D. Bernardo M. J. Claesson A. J. Lucendo Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
description	Abstract Eosinophilic esophagitis (EoE) is a chronic, immune-mediated inflammatory esophageal disease triggered by food antigens. Cumulative evidence supports the implication of microbiota and the innate immune system in the pathogenesis of EoE. Changes in the esophageal microbiome were investigated by applying 16S rRNA gene sequencing on esophageal biopsies of adult patients with active EoE at baseline (n = 30), and after achieving remission with either proton pump inhibitors (PPI, n = 10), swallowed topical corticosteroids (STC, n = 10) or food-elimination diets (FED, n = 10). Ten non-EoE biopsies were also characterized as controls. Compared to controls, no differences in alpha (intra-sample) diversity were found in EoE microbiota overall. However, it decreased significantly among patients who underwent FED. As for beta (inter-sample) diversity, non-EoE controls separated from EoE baseline samples. Post-treatment samples from patients treated with PPI and FED had a more similar microbiota composition, while those receiving STC were closer to controls. Differential testing of microbial relative abundance displayed significant changes for Filifactor, Parvimonas and Porphyromonas genera. Analysis of predicted functions indicated alterations in metabolic pathways and abundance of sulphur-cytochrome oxidoreductases. Our findings demonstrate changes in microbiota associated with EoE, as well as a treatment effect on the microbiome.
format	article
author	E. J. Laserna-Mendieta J. A. FitzGerald L. Arias-Gonzalez J. M. Ollala D. Bernardo M. J. Claesson A. J. Lucendo
author_facet	E. J. Laserna-Mendieta J. A. FitzGerald L. Arias-Gonzalez J. M. Ollala D. Bernardo M. J. Claesson A. J. Lucendo
author_sort	E. J. Laserna-Mendieta
title	Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
title_short	Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
title_full	Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
title_fullStr	Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
title_full_unstemmed	Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
title_sort	esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d705523ff13b43148c3bae3a919eeaee
work_keys_str_mv	AT ejlasernamendieta esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT jafitzgerald esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT lariasgonzalez esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT jmollala esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT dbernardo esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT mjclaesson esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies AT ajlucendo esophagealmicrobiomeinactiveeosinophilicesophagitisandchangesinducedbydifferenttherapies
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Esophageal microbiome in active eosinophilic esophagitis and changes induced by different therapies

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