Abundance and size of hyaluronan in naked mole-rat tissues and plasma

Abstract Large amounts of ultra-high molecular weight hyaluronan (HA) have been described as the main cause of cancer resistance in naked mole-rats (Heterocephalus glaber, NMR). Our work examined HA metabolism in these rodents more closely. HA was localized and quantified using HA binding proteins....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Delphine del Marmol, Susanne Holtze, Nadia Kichler, Arne Sahm, Benoit Bihin, Virginie Bourguignon, Sophie Dogné, Karol Szafranski, Thomas Bernd Hildebrandt, Bruno Flamion
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7085e041e044033971449e3ea7d50e2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d7085e041e044033971449e3ea7d50e2
record_format	dspace
spelling	oai:doaj.org-article:d7085e041e044033971449e3ea7d50e22021-12-02T14:26:12ZAbundance and size of hyaluronan in naked mole-rat tissues and plasma10.1038/s41598-021-86967-92045-2322https://doaj.org/article/d7085e041e044033971449e3ea7d50e22021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86967-9https://doaj.org/toc/2045-2322Abstract Large amounts of ultra-high molecular weight hyaluronan (HA) have been described as the main cause of cancer resistance in naked mole-rats (Heterocephalus glaber, NMR). Our work examined HA metabolism in these rodents more closely. HA was localized and quantified using HA binding proteins. Its molecular weight was determined using size exclusion chromatography and gel electrophoresis, HA family gene expression using RNAseq analysis, and hyaluronidase activity using zymography. Guinea pigs (Cavia porcellus) and mice (Mus musculus) were used as controls for some experiments. We found that HA localization was similar in NMR, guinea pig, and mouse tissues but NMR had larger amounts and higher molecular weight (maximum, around 2.5 MDa) of HA in serum and almost all tissues tested. We could not find ultra-high molecular weight HA (≥ 4 MDa) in NMR samples, in contrast to previous descriptions. Hyaluronidase-1 had lower expression and activity in NMR than mouse lymph nodes. RNAseq results showed that, among HA family genes, Tnfaip6 and hyaluronidase-3 (Hyal3) were systematically overexpressed in NMR tissues. In conclusion, NMR samples, contrary to expectations, do not harbor ultra-high molecular weight HA, although its amount and average molecular weight are higher in NMR than in guinea pig tissues and serum. Although hyaluronidase expression and activity are lower in NMR than mouse lymph nodes, this not sufficient to explain the presence of high molecular weight HA. A different activity of the NMR HA synthases remains possible. These characteristics, together with extremely high Hyal3 and Tnfaip6 expression, may provide the NMR with a bespoke, and perhaps protective, HA metabolism.Delphine del MarmolSusanne HoltzeNadia KichlerArne SahmBenoit BihinVirginie BourguignonSophie DognéKarol SzafranskiThomas Bernd HildebrandtBruno FlamionNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Delphine del Marmol Susanne Holtze Nadia Kichler Arne Sahm Benoit Bihin Virginie Bourguignon Sophie Dogné Karol Szafranski Thomas Bernd Hildebrandt Bruno Flamion Abundance and size of hyaluronan in naked mole-rat tissues and plasma
description	Abstract Large amounts of ultra-high molecular weight hyaluronan (HA) have been described as the main cause of cancer resistance in naked mole-rats (Heterocephalus glaber, NMR). Our work examined HA metabolism in these rodents more closely. HA was localized and quantified using HA binding proteins. Its molecular weight was determined using size exclusion chromatography and gel electrophoresis, HA family gene expression using RNAseq analysis, and hyaluronidase activity using zymography. Guinea pigs (Cavia porcellus) and mice (Mus musculus) were used as controls for some experiments. We found that HA localization was similar in NMR, guinea pig, and mouse tissues but NMR had larger amounts and higher molecular weight (maximum, around 2.5 MDa) of HA in serum and almost all tissues tested. We could not find ultra-high molecular weight HA (≥ 4 MDa) in NMR samples, in contrast to previous descriptions. Hyaluronidase-1 had lower expression and activity in NMR than mouse lymph nodes. RNAseq results showed that, among HA family genes, Tnfaip6 and hyaluronidase-3 (Hyal3) were systematically overexpressed in NMR tissues. In conclusion, NMR samples, contrary to expectations, do not harbor ultra-high molecular weight HA, although its amount and average molecular weight are higher in NMR than in guinea pig tissues and serum. Although hyaluronidase expression and activity are lower in NMR than mouse lymph nodes, this not sufficient to explain the presence of high molecular weight HA. A different activity of the NMR HA synthases remains possible. These characteristics, together with extremely high Hyal3 and Tnfaip6 expression, may provide the NMR with a bespoke, and perhaps protective, HA metabolism.
format	article
author	Delphine del Marmol Susanne Holtze Nadia Kichler Arne Sahm Benoit Bihin Virginie Bourguignon Sophie Dogné Karol Szafranski Thomas Bernd Hildebrandt Bruno Flamion
author_facet	Delphine del Marmol Susanne Holtze Nadia Kichler Arne Sahm Benoit Bihin Virginie Bourguignon Sophie Dogné Karol Szafranski Thomas Bernd Hildebrandt Bruno Flamion
author_sort	Delphine del Marmol
title	Abundance and size of hyaluronan in naked mole-rat tissues and plasma
title_short	Abundance and size of hyaluronan in naked mole-rat tissues and plasma
title_full	Abundance and size of hyaluronan in naked mole-rat tissues and plasma
title_fullStr	Abundance and size of hyaluronan in naked mole-rat tissues and plasma
title_full_unstemmed	Abundance and size of hyaluronan in naked mole-rat tissues and plasma
title_sort	abundance and size of hyaluronan in naked mole-rat tissues and plasma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7085e041e044033971449e3ea7d50e2
work_keys_str_mv	AT delphinedelmarmol abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT susanneholtze abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT nadiakichler abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT arnesahm abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT benoitbihin abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT virginiebourguignon abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT sophiedogne abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT karolszafranski abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT thomasberndhildebrandt abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma AT brunoflamion abundanceandsizeofhyaluronaninnakedmolerattissuesandplasma
_version_	1718391399668252672

Abundance and size of hyaluronan in naked mole-rat tissues and plasma

Ejemplares similares