Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.

We used RNAseq to generate a comprehensive transcriptome of Brown Adipose Tissue (BAT) over the course of a year in the naturally hibernating thirteen-lined ground squirrel, Ictidomys tridecemlineatus. During hibernation ground squirrels do not feed and use fat stored in White Adipose Tissue (WAT) a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marshall Hampton, Richard G Melvin, Matthew T Andrews
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d70b5aa567a04934a1c0f4a5fcee5071
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d70b5aa567a04934a1c0f4a5fcee5071
record_format	dspace
spelling	oai:doaj.org-article:d70b5aa567a04934a1c0f4a5fcee50712021-11-18T08:39:44ZTranscriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.1932-620310.1371/journal.pone.0085157https://doaj.org/article/d70b5aa567a04934a1c0f4a5fcee50712013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386461/?tool=EBIhttps://doaj.org/toc/1932-6203We used RNAseq to generate a comprehensive transcriptome of Brown Adipose Tissue (BAT) over the course of a year in the naturally hibernating thirteen-lined ground squirrel, Ictidomys tridecemlineatus. During hibernation ground squirrels do not feed and use fat stored in White Adipose Tissue (WAT) as their primary source of fuel. Stored lipid is consumed at high rates by BAT to generate heat at specific points during the hibernation season. The highest rate of BAT activity occurs during periodic arousals from hypothermic torpor bouts, referred to as Interbout Arousals (IBAs). IBAs are characterized by whole body re-warming (from 5 to 37 °C) in 2-3 hours, and provide a unique opportunity to determine the genes responsible for the highly efficient lipid oxidation and heat generation that drives the arousal process. Illumina HighSeq sequencing identified 14,573 distinct BAT mRNAs and quantified their levels at four points: active ground squirrels in April and October, and hibernating animals during both torpor and IBA. Based on significant changes in mRNA levels across the four collection points, 2,083 genes were shown to be differentially expressed. In addition to providing detail on the expression of nuclear genes encoding mitochondrial proteins, and genes involved in beta-adrenergic and lipolytic pathways, we identified differentially expressed genes encoding various transcription factors and other regulatory proteins which may play critical roles in high efficiency fat catabolism, non-shivering thermogenesis, and transitions into and out of the torpid state.Marshall HamptonRichard G MelvinMatthew T AndrewsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e85157 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marshall Hampton Richard G Melvin Matthew T Andrews Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
description	We used RNAseq to generate a comprehensive transcriptome of Brown Adipose Tissue (BAT) over the course of a year in the naturally hibernating thirteen-lined ground squirrel, Ictidomys tridecemlineatus. During hibernation ground squirrels do not feed and use fat stored in White Adipose Tissue (WAT) as their primary source of fuel. Stored lipid is consumed at high rates by BAT to generate heat at specific points during the hibernation season. The highest rate of BAT activity occurs during periodic arousals from hypothermic torpor bouts, referred to as Interbout Arousals (IBAs). IBAs are characterized by whole body re-warming (from 5 to 37 °C) in 2-3 hours, and provide a unique opportunity to determine the genes responsible for the highly efficient lipid oxidation and heat generation that drives the arousal process. Illumina HighSeq sequencing identified 14,573 distinct BAT mRNAs and quantified their levels at four points: active ground squirrels in April and October, and hibernating animals during both torpor and IBA. Based on significant changes in mRNA levels across the four collection points, 2,083 genes were shown to be differentially expressed. In addition to providing detail on the expression of nuclear genes encoding mitochondrial proteins, and genes involved in beta-adrenergic and lipolytic pathways, we identified differentially expressed genes encoding various transcription factors and other regulatory proteins which may play critical roles in high efficiency fat catabolism, non-shivering thermogenesis, and transitions into and out of the torpid state.
format	article
author	Marshall Hampton Richard G Melvin Matthew T Andrews
author_facet	Marshall Hampton Richard G Melvin Matthew T Andrews
author_sort	Marshall Hampton
title	Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
title_short	Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
title_full	Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
title_fullStr	Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
title_full_unstemmed	Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
title_sort	transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d70b5aa567a04934a1c0f4a5fcee5071
work_keys_str_mv	AT marshallhampton transcriptomicanalysisofbrownadiposetissueacrossthephysiologicalextremesofnaturalhibernation AT richardgmelvin transcriptomicanalysisofbrownadiposetissueacrossthephysiologicalextremesofnaturalhibernation AT matthewtandrews transcriptomicanalysisofbrownadiposetissueacrossthephysiologicalextremesofnaturalhibernation
_version_	1718421529922895872

Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation.

Ejemplares similares