Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation

Abstract Recent trials demonstrate that systemic anti-inflammatory therapy reduces cardiovascular events in coronary artery disease (CAD) patients. We recently demonstrated Lactobacillus plantarum 299v (Lp299v) supplementation improved vascular endothelial function in men with stable CAD. Whether th...

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Autores principales:	Benjamin C. Hofeld, Venkata K. Puppala, Sudhi Tyagi, Kwang Woo Ahn, Amberly Anger, Shuang Jia, Nita H. Salzman, Martin J. Hessner, Michael E. Widlansky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7166fea3e3149f68f5acd9747714a2b
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spelling	oai:doaj.org-article:d7166fea3e3149f68f5acd9747714a2b2021-12-02T12:11:45ZLactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation10.1038/s41598-021-83252-72045-2322https://doaj.org/article/d7166fea3e3149f68f5acd9747714a2b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83252-7https://doaj.org/toc/2045-2322Abstract Recent trials demonstrate that systemic anti-inflammatory therapy reduces cardiovascular events in coronary artery disease (CAD) patients. We recently demonstrated Lactobacillus plantarum 299v (Lp299v) supplementation improved vascular endothelial function in men with stable CAD. Whether this favorable effect is in part due to anti-inflammatory action remains unknown. Testing this hypothesis, we exposed plasma obtained before and after Lp299v supplementation from these subjects to a healthy donor’s PBMCs and measured differences in the PBMC transciptome, performed gene ontological analyses, and compared Lp299v-induced transcriptome changes with changes in vascular function. Daily alcohol users (DAUs) (n = 4) had a significantly different response to Lp299v and were separated from the main analyses. Non-DAUs- (n = 15) showed improved brachial flow-mediated dilation (FMD) and reduced circulating IL-8, IL-12, and leptin. 997 genes were significantly changed. I.I.com decreased (1.01 ± 0.74 vs. 0.22 ± 0.51; P < 0.0001), indicating strong anti-inflammatory effects. Pathway analyses revealed downregulation of IL-1β, interferon-stimulated pathways, and toll-like receptor signaling, and an increase in regulator T-cell (Treg) activity. Reductions in GBP1, JAK2, and TRAIL expression correlated with improved FMD. In non-DAU men with stable CAD, post-Lp299v supplementation plasma induced anti-inflammatory transcriptome changes in human PBMCs that could benefit CAD patients. Future studies should delineate changes in circulating metabolites responsible for these effects.Benjamin C. HofeldVenkata K. PuppalaSudhi TyagiKwang Woo AhnAmberly AngerShuang JiaNita H. SalzmanMartin J. HessnerMichael E. WidlanskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benjamin C. Hofeld Venkata K. Puppala Sudhi Tyagi Kwang Woo Ahn Amberly Anger Shuang Jia Nita H. Salzman Martin J. Hessner Michael E. Widlansky Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
description	Abstract Recent trials demonstrate that systemic anti-inflammatory therapy reduces cardiovascular events in coronary artery disease (CAD) patients. We recently demonstrated Lactobacillus plantarum 299v (Lp299v) supplementation improved vascular endothelial function in men with stable CAD. Whether this favorable effect is in part due to anti-inflammatory action remains unknown. Testing this hypothesis, we exposed plasma obtained before and after Lp299v supplementation from these subjects to a healthy donor’s PBMCs and measured differences in the PBMC transciptome, performed gene ontological analyses, and compared Lp299v-induced transcriptome changes with changes in vascular function. Daily alcohol users (DAUs) (n = 4) had a significantly different response to Lp299v and were separated from the main analyses. Non-DAUs- (n = 15) showed improved brachial flow-mediated dilation (FMD) and reduced circulating IL-8, IL-12, and leptin. 997 genes were significantly changed. I.I.com decreased (1.01 ± 0.74 vs. 0.22 ± 0.51; P < 0.0001), indicating strong anti-inflammatory effects. Pathway analyses revealed downregulation of IL-1β, interferon-stimulated pathways, and toll-like receptor signaling, and an increase in regulator T-cell (Treg) activity. Reductions in GBP1, JAK2, and TRAIL expression correlated with improved FMD. In non-DAU men with stable CAD, post-Lp299v supplementation plasma induced anti-inflammatory transcriptome changes in human PBMCs that could benefit CAD patients. Future studies should delineate changes in circulating metabolites responsible for these effects.
format	article
author	Benjamin C. Hofeld Venkata K. Puppala Sudhi Tyagi Kwang Woo Ahn Amberly Anger Shuang Jia Nita H. Salzman Martin J. Hessner Michael E. Widlansky
author_facet	Benjamin C. Hofeld Venkata K. Puppala Sudhi Tyagi Kwang Woo Ahn Amberly Anger Shuang Jia Nita H. Salzman Martin J. Hessner Michael E. Widlansky
author_sort	Benjamin C. Hofeld
title	Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
title_short	Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
title_full	Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
title_fullStr	Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
title_full_unstemmed	Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
title_sort	lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7166fea3e3149f68f5acd9747714a2b
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Lactobacillus plantarum 299v probiotic supplementation in men with stable coronary artery disease suppresses systemic inflammation

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