Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

While the analysis of SARS-CoV-2 RNA in stool samples has led to important insights regarding the disease, quantification is currently challenging. Here the authors use patient samples to benchmark preservation, extraction and quantification methods to optimise detection of viral RNA.

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Autores principales: Aravind Natarajan, Alvin Han, Soumaya Zlitni, Erin F. Brooks, Summer E. Vance, Marlene Wolfe, Upinder Singh, Prasanna Jagannathan, Benjamin A. Pinsky, Alexandria Boehm, Ami S. Bhatt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/d728658748924f6fab1daf529898ddfd
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spelling oai:doaj.org-article:d728658748924f6fab1daf529898ddfd2021-12-02T17:37:42ZStandardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA10.1038/s41467-021-25576-62041-1723https://doaj.org/article/d728658748924f6fab1daf529898ddfd2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25576-6https://doaj.org/toc/2041-1723While the analysis of SARS-CoV-2 RNA in stool samples has led to important insights regarding the disease, quantification is currently challenging. Here the authors use patient samples to benchmark preservation, extraction and quantification methods to optimise detection of viral RNA.Aravind NatarajanAlvin HanSoumaya ZlitniErin F. BrooksSummer E. VanceMarlene WolfeUpinder SinghPrasanna JagannathanBenjamin A. PinskyAlexandria BoehmAmi S. BhattNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Aravind Natarajan
Alvin Han
Soumaya Zlitni
Erin F. Brooks
Summer E. Vance
Marlene Wolfe
Upinder Singh
Prasanna Jagannathan
Benjamin A. Pinsky
Alexandria Boehm
Ami S. Bhatt
Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
description While the analysis of SARS-CoV-2 RNA in stool samples has led to important insights regarding the disease, quantification is currently challenging. Here the authors use patient samples to benchmark preservation, extraction and quantification methods to optimise detection of viral RNA.
format article
author Aravind Natarajan
Alvin Han
Soumaya Zlitni
Erin F. Brooks
Summer E. Vance
Marlene Wolfe
Upinder Singh
Prasanna Jagannathan
Benjamin A. Pinsky
Alexandria Boehm
Ami S. Bhatt
author_facet Aravind Natarajan
Alvin Han
Soumaya Zlitni
Erin F. Brooks
Summer E. Vance
Marlene Wolfe
Upinder Singh
Prasanna Jagannathan
Benjamin A. Pinsky
Alexandria Boehm
Ami S. Bhatt
author_sort Aravind Natarajan
title Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
title_short Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
title_full Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
title_fullStr Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
title_full_unstemmed Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
title_sort standardized preservation, extraction and quantification techniques for detection of fecal sars-cov-2 rna
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/d728658748924f6fab1daf529898ddfd
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