Evaluation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in Pyrus pyrifolia using different tissue samples and seasonal conditions.
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α, Glyceraldehyde-3-phosphate dehydrogenase, together wit...
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Auteurs principaux: | Tsuyoshi Imai, Benjamin E Ubi, Takanori Saito, Takaya Moriguchi |
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Format: | article |
Langue: | EN |
Publié: |
Public Library of Science (PLoS)
2014
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Accès en ligne: | https://doaj.org/article/d72d7c0e7e3043c4b93771593da45d44 |
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