High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain

The prevalence of <i>Escherichia coli</i> was analysed in poultry products from different Spanish retailers and determined its antibiotic resistance capability by phenotypic (ampicillin, amoxicillin, chloramphenicol, gentamicin, imipenem, cefotaxime, tetracycline, ciprofloxacin, trimetho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Beatriz García-Béjar, Izan García de Blas Martín, María Arévalo-Villena, Ana Briones Pérez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotic resistance enterobacteriaceae <i>Escherichia coli</i> poultry products retailers Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/d72dff633e8447be9603f408a2f6f705
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d72dff633e8447be9603f408a2f6f705
record_format	dspace
spelling	oai:doaj.org-article:d72dff633e8447be9603f408a2f6f7052021-11-25T16:18:51ZHigh Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain10.3390/ani111131972076-2615https://doaj.org/article/d72dff633e8447be9603f408a2f6f7052021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3197https://doaj.org/toc/2076-2615The prevalence of <i>Escherichia coli</i> was analysed in poultry products from different Spanish retailers and determined its antibiotic resistance capability by phenotypic (ampicillin, amoxicillin, chloramphenicol, gentamicin, imipenem, cefotaxime, tetracycline, ciprofloxacin, trimethoprim, and colistin) and genotypic assays. A total of 30 samples (hindquarters or livers) were collected from supermarkets and butchers. Enterobacteriaceae counts ranged between 3.2 and 6.5 log colony-forming units (CFU)/g, and the highest values were found in livers and in samples from supermarkets. <i>E. coli</i> was detected in 83% of the samples tested, and the highest prevalence was observed in livers (100%) and supermarkets (91%). Regarding the antibiotic sensitivity test, 100% of the <i>E. coli</i> showed resistance to at least one antibiotic. The highest resistance rates were detected for colistin (87%) and gentamicin (79%), while only two antibiotics (chloramphenicol and cefotaxime) showed a resistance lower than 10%. Furthermore, the resistance genes of tetracycline and beta-lactams were analysed by multiplex PCR, revealing that <i>tet</i>(<i>A</i>) and <i>blaTEM</i> were the majority genes, respectively.Beatriz García-BéjarIzan García de Blas MartínMaría Arévalo-VillenaAna Briones PérezMDPI AGarticleantibiotic resistanceenterobacteriaceae<i>Escherichia coli</i>poultry productsretailersVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3197, p 3197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance enterobacteriaceae <i>Escherichia coli</i> poultry products retailers Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	antibiotic resistance enterobacteriaceae <i>Escherichia coli</i> poultry products retailers Veterinary medicine SF600-1100 Zoology QL1-991 Beatriz García-Béjar Izan García de Blas Martín María Arévalo-Villena Ana Briones Pérez High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
description	The prevalence of <i>Escherichia coli</i> was analysed in poultry products from different Spanish retailers and determined its antibiotic resistance capability by phenotypic (ampicillin, amoxicillin, chloramphenicol, gentamicin, imipenem, cefotaxime, tetracycline, ciprofloxacin, trimethoprim, and colistin) and genotypic assays. A total of 30 samples (hindquarters or livers) were collected from supermarkets and butchers. Enterobacteriaceae counts ranged between 3.2 and 6.5 log colony-forming units (CFU)/g, and the highest values were found in livers and in samples from supermarkets. <i>E. coli</i> was detected in 83% of the samples tested, and the highest prevalence was observed in livers (100%) and supermarkets (91%). Regarding the antibiotic sensitivity test, 100% of the <i>E. coli</i> showed resistance to at least one antibiotic. The highest resistance rates were detected for colistin (87%) and gentamicin (79%), while only two antibiotics (chloramphenicol and cefotaxime) showed a resistance lower than 10%. Furthermore, the resistance genes of tetracycline and beta-lactams were analysed by multiplex PCR, revealing that <i>tet</i>(<i>A</i>) and <i>blaTEM</i> were the majority genes, respectively.
format	article
author	Beatriz García-Béjar Izan García de Blas Martín María Arévalo-Villena Ana Briones Pérez
author_facet	Beatriz García-Béjar Izan García de Blas Martín María Arévalo-Villena Ana Briones Pérez
author_sort	Beatriz García-Béjar
title	High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
title_short	High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
title_full	High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
title_fullStr	High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
title_full_unstemmed	High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain
title_sort	high prevalence of antibiotic-resistant <i>escherichia coli</i> isolates from retail poultry products in spain
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d72dff633e8447be9603f408a2f6f705
work_keys_str_mv	AT beatrizgarciabejar highprevalenceofantibioticresistantiescherichiacoliiisolatesfromretailpoultryproductsinspain AT izangarciadeblasmartin highprevalenceofantibioticresistantiescherichiacoliiisolatesfromretailpoultryproductsinspain AT mariaarevalovillena highprevalenceofantibioticresistantiescherichiacoliiisolatesfromretailpoultryproductsinspain AT anabrionesperez highprevalenceofantibioticresistantiescherichiacoliiisolatesfromretailpoultryproductsinspain
_version_	1718413285304303616

High Prevalence of Antibiotic-Resistant <i>Escherichia coli</i> Isolates from Retail Poultry Products in Spain

Ejemplares similares