CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells

Abstract Angiogenesis is the process by which new vessels form from existing vascular networks. Human umbilical vein endothelial cells (HUVECs) may contribute to the study of vascular repair and angiogenesis. The chemokine CXCL12 regulates multiple cell functions, including angiogenesis, mainly thro...

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Autores principales:	Min Zhang, Lisha Qiu, Yanyan Zhang, Dongsheng Xu, Jialin C. Zheng, Li Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7352edfa3f743dfacbb77c34bfc3a60
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spelling	oai:doaj.org-article:d7352edfa3f743dfacbb77c34bfc3a602021-12-02T15:05:24ZCXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells10.1038/s41598-017-08840-y2045-2322https://doaj.org/article/d7352edfa3f743dfacbb77c34bfc3a602017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08840-yhttps://doaj.org/toc/2045-2322Abstract Angiogenesis is the process by which new vessels form from existing vascular networks. Human umbilical vein endothelial cells (HUVECs) may contribute to the study of vascular repair and angiogenesis. The chemokine CXCL12 regulates multiple cell functions, including angiogenesis, mainly through its receptor CXCR4. In contrast to CXCL12/CXCR4, few studies have described roles for CXCR7 in vascular biology, and the downstream mechanism of CXCR7 in angiogenesis remains unclear. The results of the present study showed that CXCL12 dose-dependently enhanced angiogenesis in chorioallantoic membranes (CAMs) and HUVECs. The specific activation of CXCR7 with TC14012 (a CXCR7 agonist) resulted in the significant induction of tube formation in HUVECs and in vivo. Further evidence suggested that CXCL12 induced directional polarization and migration in the HUVECs, which is necessary for tube formation. Moreover, CXCR7 translocalization was observed during the polarization of HUVECs in stripe assays. Finally, treatment with TC14012 also significantly increased PI3K/Akt phosphorylation, and tube formation was blocked by treating HUVECs with an Akt inhibitor. Overall, this study indicated that CXCL12-stimulated CXCR7 acts as a functional receptor to activate Akt for angiogenesis in HUVECs and that CXCR7 may be a potential target molecule for endothelial regeneration and repair after vascular injury.Min ZhangLisha QiuYanyan ZhangDongsheng XuJialin C. ZhengLi JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Zhang Lisha Qiu Yanyan Zhang Dongsheng Xu Jialin C. Zheng Li Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
description	Abstract Angiogenesis is the process by which new vessels form from existing vascular networks. Human umbilical vein endothelial cells (HUVECs) may contribute to the study of vascular repair and angiogenesis. The chemokine CXCL12 regulates multiple cell functions, including angiogenesis, mainly through its receptor CXCR4. In contrast to CXCL12/CXCR4, few studies have described roles for CXCR7 in vascular biology, and the downstream mechanism of CXCR7 in angiogenesis remains unclear. The results of the present study showed that CXCL12 dose-dependently enhanced angiogenesis in chorioallantoic membranes (CAMs) and HUVECs. The specific activation of CXCR7 with TC14012 (a CXCR7 agonist) resulted in the significant induction of tube formation in HUVECs and in vivo. Further evidence suggested that CXCL12 induced directional polarization and migration in the HUVECs, which is necessary for tube formation. Moreover, CXCR7 translocalization was observed during the polarization of HUVECs in stripe assays. Finally, treatment with TC14012 also significantly increased PI3K/Akt phosphorylation, and tube formation was blocked by treating HUVECs with an Akt inhibitor. Overall, this study indicated that CXCL12-stimulated CXCR7 acts as a functional receptor to activate Akt for angiogenesis in HUVECs and that CXCR7 may be a potential target molecule for endothelial regeneration and repair after vascular injury.
format	article
author	Min Zhang Lisha Qiu Yanyan Zhang Dongsheng Xu Jialin C. Zheng Li Jiang
author_facet	Min Zhang Lisha Qiu Yanyan Zhang Dongsheng Xu Jialin C. Zheng Li Jiang
author_sort	Min Zhang
title	CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
title_short	CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
title_full	CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
title_fullStr	CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
title_full_unstemmed	CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
title_sort	cxcl12 enhances angiogenesis through cxcr7 activation in human umbilical vein endothelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d7352edfa3f743dfacbb77c34bfc3a60
work_keys_str_mv	AT minzhang cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells AT lishaqiu cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells AT yanyanzhang cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells AT dongshengxu cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells AT jialinczheng cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells AT lijiang cxcl12enhancesangiogenesisthroughcxcr7activationinhumanumbilicalveinendothelialcells
_version_	1718388860043395072

CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells

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