Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria

Introduction. The functional basis of protopectin complex can be represented as a network of regions that consist of homogalacturonan sequences and a base of rhamnogalacturonans-I, i.e. rhamnosyl-containing branching sites. Enzymatic isolation of these regions is possible only at a certain minimal...

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Autores principales:	Vladimir V. Kondratenko, Tatyana Yu. Kondratenko, Andrey N. Petrov
Formato:	article
Lenguaje:	EN
Publicado:	Kemerovo State University 2021
Materias:	protopectin complex rhamnogalacturonan-i homogalacturonan transformation criterion assessment Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/d738baaa74804cbb99c148c09236f4be
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spelling	oai:doaj.org-article:d738baaa74804cbb99c148c09236f4be2021-11-19T04:02:56ZDirected homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria2308-40572310-959910.21603/2308-4057-2021-2-254-261https://doaj.org/article/d738baaa74804cbb99c148c09236f4be2021-11-01T00:00:00Zhttp://jfrm.ru/en/issues/1879/1908/https://doaj.org/toc/2308-4057https://doaj.org/toc/2310-9599Introduction. The functional basis of protopectin complex can be represented as a network of regions that consist of homogalacturonan sequences and a base of rhamnogalacturonans-I, i.e. rhamnosyl-containing branching sites. Enzymatic isolation of these regions is possible only at a certain minimal native degree of polymerization. The research objective was to develop a system of criteria for assessing the potential applicability of the enzymatic transformation of plant protopectin complex. Study objects and methods. The research featured the polymerization degree of the homogalacturonan regions of the protopectin complex and produced a system of assessment criteria for the enzymatic fragmentation potential of the protopectin complex. The theoretical calculations were based on the values of the mass fractions of rhamnosyl and galacturonide residues in plant cell walls. The result was a new polymerization degree analytical function. Results and discussion. The ratio of the mass fractions of rhamnosyl and galacturonide residues in the water-insoluble plant tissue served as a dimensionless criterion of applicability. The rational condition for the dimensionless criterion of applicability was based on the fundamental constraint for homogalacturonan regions in the protopectin complex. It was expressed by a fundamental inequation. The rational area for determining the numerical values of the applicability criterion was presented as . The functional dependence was reduced to a two-dimensional criteria space as “width of rhamnosyl branches vs. the criterion of applicability”, where each pectin-containing raw material was given a single uniquely defined position. The boundary conditions for the criteria space were determined analytically. Conclusion. The new approach offers an assessment of the enzymatic fragmentation potential of the plant protopectin complex by homoenzyme preparations. The approach is in fact the second stage of the decision tree in the science-based technology for pectin and its products.Vladimir V. KondratenkoTatyana Yu. KondratenkoAndrey N. PetrovKemerovo State Universityarticleprotopectin complexrhamnogalacturonan-ihomogalacturonantransformationcriterion assessmentFood processing and manufactureTP368-456ENFoods and Raw Materials, Vol 9, Iss 2, Pp 254-261 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	protopectin complex rhamnogalacturonan-i homogalacturonan transformation criterion assessment Food processing and manufacture TP368-456
spellingShingle	protopectin complex rhamnogalacturonan-i homogalacturonan transformation criterion assessment Food processing and manufacture TP368-456 Vladimir V. Kondratenko Tatyana Yu. Kondratenko Andrey N. Petrov Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
description	Introduction. The functional basis of protopectin complex can be represented as a network of regions that consist of homogalacturonan sequences and a base of rhamnogalacturonans-I, i.e. rhamnosyl-containing branching sites. Enzymatic isolation of these regions is possible only at a certain minimal native degree of polymerization. The research objective was to develop a system of criteria for assessing the potential applicability of the enzymatic transformation of plant protopectin complex. Study objects and methods. The research featured the polymerization degree of the homogalacturonan regions of the protopectin complex and produced a system of assessment criteria for the enzymatic fragmentation potential of the protopectin complex. The theoretical calculations were based on the values of the mass fractions of rhamnosyl and galacturonide residues in plant cell walls. The result was a new polymerization degree analytical function. Results and discussion. The ratio of the mass fractions of rhamnosyl and galacturonide residues in the water-insoluble plant tissue served as a dimensionless criterion of applicability. The rational condition for the dimensionless criterion of applicability was based on the fundamental constraint for homogalacturonan regions in the protopectin complex. It was expressed by a fundamental inequation. The rational area for determining the numerical values of the applicability criterion was presented as . The functional dependence was reduced to a two-dimensional criteria space as “width of rhamnosyl branches vs. the criterion of applicability”, where each pectin-containing raw material was given a single uniquely defined position. The boundary conditions for the criteria space were determined analytically. Conclusion. The new approach offers an assessment of the enzymatic fragmentation potential of the plant protopectin complex by homoenzyme preparations. The approach is in fact the second stage of the decision tree in the science-based technology for pectin and its products.
format	article
author	Vladimir V. Kondratenko Tatyana Yu. Kondratenko Andrey N. Petrov
author_facet	Vladimir V. Kondratenko Tatyana Yu. Kondratenko Andrey N. Petrov
author_sort	Vladimir V. Kondratenko
title	Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
title_short	Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
title_full	Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
title_fullStr	Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
title_full_unstemmed	Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria
title_sort	directed homoenzymatic fragmentation of the plant protopectin complex: assessment criteria
publisher	Kemerovo State University
publishDate	2021
url	https://doaj.org/article/d738baaa74804cbb99c148c09236f4be
work_keys_str_mv	AT vladimirvkondratenko directedhomoenzymaticfragmentationoftheplantprotopectincomplexassessmentcriteria AT tatyanayukondratenko directedhomoenzymaticfragmentationoftheplantprotopectincomplexassessmentcriteria AT andreynpetrov directedhomoenzymaticfragmentationoftheplantprotopectincomplexassessmentcriteria
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Directed homoenzymatic fragmentation of the plant protopectin complex: Assessment criteria

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