MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation

Abstract During pregnancy, the maternal immune system must tolerate the developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on the MR1 molecule, but their pres...

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Autores principales:	Martin Solders, Laia Gorchs, Tom Erkers, Anna-Carin Lundell, Silvia Nava, Sebastian Gidlöf, Eleonor Tiblad, Isabelle Magalhaes, Helen Kaipe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d73deead6e84476ebda71dc86fb4d4a7
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spelling	oai:doaj.org-article:d73deead6e84476ebda71dc86fb4d4a72021-12-02T12:30:43ZMAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation10.1038/s41598-017-06430-62045-2322https://doaj.org/article/d73deead6e84476ebda71dc86fb4d4a72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06430-6https://doaj.org/toc/2045-2322Abstract During pregnancy, the maternal immune system must tolerate the developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on the MR1 molecule, but their presence and function at the foetal-maternal interface is not known. We here isolated mononuclear cells from paired samples of peripheral blood (PB), intervillous blood (IVB), and decidua parietalis (DP) following uncomplicated term pregnancies. Interestingly, MAIT cells were highly enriched in IVB compared to PB and DP. The activation status of IVB MAIT cells was similar to that of PB MAIT cells, except for a lower expression of PD-1. Both IVB MAIT cells and conventional T cells were more dominated by an effector memory phenotype compared to PB MAIT cells and T cells. IVB MAIT cells also responded more vigorously with expression of IFN-γ, granzyme B, and perforin in response to Escherichia coli stimulation compared to PB. MR1 was not expressed in syncytiotrophoblasts, but in placental villous and decidual macrophages. These data indicate that maternal MAIT cells accumulate in the intervillous space of the placenta and that they are highly armed to quickly respond if bacteria are encountered at the foetal-maternal interface.Martin SoldersLaia GorchsTom ErkersAnna-Carin LundellSilvia NavaSebastian GidlöfEleonor TibladIsabelle MagalhaesHelen KaipeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Solders Laia Gorchs Tom Erkers Anna-Carin Lundell Silvia Nava Sebastian Gidlöf Eleonor Tiblad Isabelle Magalhaes Helen Kaipe MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
description	Abstract During pregnancy, the maternal immune system must tolerate the developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on the MR1 molecule, but their presence and function at the foetal-maternal interface is not known. We here isolated mononuclear cells from paired samples of peripheral blood (PB), intervillous blood (IVB), and decidua parietalis (DP) following uncomplicated term pregnancies. Interestingly, MAIT cells were highly enriched in IVB compared to PB and DP. The activation status of IVB MAIT cells was similar to that of PB MAIT cells, except for a lower expression of PD-1. Both IVB MAIT cells and conventional T cells were more dominated by an effector memory phenotype compared to PB MAIT cells and T cells. IVB MAIT cells also responded more vigorously with expression of IFN-γ, granzyme B, and perforin in response to Escherichia coli stimulation compared to PB. MR1 was not expressed in syncytiotrophoblasts, but in placental villous and decidual macrophages. These data indicate that maternal MAIT cells accumulate in the intervillous space of the placenta and that they are highly armed to quickly respond if bacteria are encountered at the foetal-maternal interface.
format	article
author	Martin Solders Laia Gorchs Tom Erkers Anna-Carin Lundell Silvia Nava Sebastian Gidlöf Eleonor Tiblad Isabelle Magalhaes Helen Kaipe
author_facet	Martin Solders Laia Gorchs Tom Erkers Anna-Carin Lundell Silvia Nava Sebastian Gidlöf Eleonor Tiblad Isabelle Magalhaes Helen Kaipe
author_sort	Martin Solders
title	MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
title_short	MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
title_full	MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
title_fullStr	MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
title_full_unstemmed	MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
title_sort	mait cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d73deead6e84476ebda71dc86fb4d4a7
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MAIT cells accumulate in placental intervillous space and display a highly cytotoxic phenotype upon bacterial stimulation

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