Carbon Dots’ Antiviral Functions Against Noroviruses

Abstract This study reported the first assessment of carbon dots’ (CDots) antiviral activity to human norovirus virus-like-particles (VLPs), GI.1 and GII.4 VLPs. CDots with different surface passivation molecules, 2,2′-(ethylenedioxy)bis(ethylamine) (EDA)-CDots and 3-ethoxypropylamine (EPA)-CDots, w...

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Autores principales:	Xiuli Dong, Marsha M. Moyer, Fan Yang, Ya-Ping Sun, Liju Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d74d13a7fe62424a9a3ccc31cc69a865
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spelling	oai:doaj.org-article:d74d13a7fe62424a9a3ccc31cc69a8652021-12-02T16:06:29ZCarbon Dots’ Antiviral Functions Against Noroviruses10.1038/s41598-017-00675-x2045-2322https://doaj.org/article/d74d13a7fe62424a9a3ccc31cc69a8652017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00675-xhttps://doaj.org/toc/2045-2322Abstract This study reported the first assessment of carbon dots’ (CDots) antiviral activity to human norovirus virus-like-particles (VLPs), GI.1 and GII.4 VLPs. CDots with different surface passivation molecules, 2,2′-(ethylenedioxy)bis(ethylamine) (EDA)-CDots and 3-ethoxypropylamine (EPA)-CDots, were synthesized and evaluated. The results indicated both EDA- and EPA- CDots were highly effective to inhibit both strains of VLPs’ bindings to histo-blood group antigens (HBGA) receptors on human cells at CDots concentration of 5 µg/mL, with EDA-CDots achieving 100% inhibition and EPA CDots achieving 85–99% inhibition. At low CDots concentration (2 µg/mL), positively charged EDA-CDots exhibited higher inhibitory effect (~82%) than non-charged EPA-CDots (~60%), suggesting the surface charge status of CDots played a role in the interactions between CDots and the negatively charged VLPs. Both types of CDots also exhibited inhibitory effect on VLP’s binding to their respective antibodies, but much less effective than those to HBGA binding. After CDots treatments, VLPs remained intact, and no degradation was observed on VLPs’ capsid proteins. Taken together, the observed antiviral effects of CDots on noroviruses were mainly through the effective inhibition of VLPs’ binding to HBGA receptors and moderate inhibition of VLPs’ binding to their antibodies, without affecting the integrity of viral capsid protein and the viral particle.Xiuli DongMarsha M. MoyerFan YangYa-Ping SunLiju YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiuli Dong Marsha M. Moyer Fan Yang Ya-Ping Sun Liju Yang Carbon Dots’ Antiviral Functions Against Noroviruses
description	Abstract This study reported the first assessment of carbon dots’ (CDots) antiviral activity to human norovirus virus-like-particles (VLPs), GI.1 and GII.4 VLPs. CDots with different surface passivation molecules, 2,2′-(ethylenedioxy)bis(ethylamine) (EDA)-CDots and 3-ethoxypropylamine (EPA)-CDots, were synthesized and evaluated. The results indicated both EDA- and EPA- CDots were highly effective to inhibit both strains of VLPs’ bindings to histo-blood group antigens (HBGA) receptors on human cells at CDots concentration of 5 µg/mL, with EDA-CDots achieving 100% inhibition and EPA CDots achieving 85–99% inhibition. At low CDots concentration (2 µg/mL), positively charged EDA-CDots exhibited higher inhibitory effect (~82%) than non-charged EPA-CDots (~60%), suggesting the surface charge status of CDots played a role in the interactions between CDots and the negatively charged VLPs. Both types of CDots also exhibited inhibitory effect on VLP’s binding to their respective antibodies, but much less effective than those to HBGA binding. After CDots treatments, VLPs remained intact, and no degradation was observed on VLPs’ capsid proteins. Taken together, the observed antiviral effects of CDots on noroviruses were mainly through the effective inhibition of VLPs’ binding to HBGA receptors and moderate inhibition of VLPs’ binding to their antibodies, without affecting the integrity of viral capsid protein and the viral particle.
format	article
author	Xiuli Dong Marsha M. Moyer Fan Yang Ya-Ping Sun Liju Yang
author_facet	Xiuli Dong Marsha M. Moyer Fan Yang Ya-Ping Sun Liju Yang
author_sort	Xiuli Dong
title	Carbon Dots’ Antiviral Functions Against Noroviruses
title_short	Carbon Dots’ Antiviral Functions Against Noroviruses
title_full	Carbon Dots’ Antiviral Functions Against Noroviruses
title_fullStr	Carbon Dots’ Antiviral Functions Against Noroviruses
title_full_unstemmed	Carbon Dots’ Antiviral Functions Against Noroviruses
title_sort	carbon dots’ antiviral functions against noroviruses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d74d13a7fe62424a9a3ccc31cc69a865
work_keys_str_mv	AT xiulidong carbondotsantiviralfunctionsagainstnoroviruses AT marshammoyer carbondotsantiviralfunctionsagainstnoroviruses AT fanyang carbondotsantiviralfunctionsagainstnoroviruses AT yapingsun carbondotsantiviralfunctionsagainstnoroviruses AT lijuyang carbondotsantiviralfunctionsagainstnoroviruses
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Carbon Dots’ Antiviral Functions Against Noroviruses

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