Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model

BACKGROUND AND OBJECTIVE: Human umbilical cord blood (HUCB) is a rich source of stem cells. The aim of this study was the investigation of the therapeutic effects of these cells on cerebral ischemia in rat. METHODS: This study was carried out on 30 male Wistar rats (100-150 g). They were divided int...

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Autores principales:	MR Nikravesh, H Ghaffaripour, M Jalali, D Hamidi Alamdari, J Sanchooli, M Seghatoleslam
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	stem cell hypoxic ischemia brain damage Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d750851cec0b454ba35b8eaad01b9d73
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spelling	oai:doaj.org-article:d750851cec0b454ba35b8eaad01b9d732021-11-10T08:53:19ZTherapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model1561-41072251-7170https://doaj.org/article/d750851cec0b454ba35b8eaad01b9d732012-07-01T00:00:00Zhttp://jbums.org/article-1-4149-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Human umbilical cord blood (HUCB) is a rich source of stem cells. The aim of this study was the investigation of the therapeutic effects of these cells on cerebral ischemia in rat. METHODS: This study was carried out on 30 male Wistar rats (100-150 g). They were divided into three groups. Firstly, to create a laboratory model of ischemic stroke, right carotid artery of animals was occluded for 30 minutes in two groups of experimental and sham. Then, mononuclear cells of cord blood were isolated and labeled using bromodeoxyuridine (BrdU) and then 2×105 cells were injected into the experimental group via the tail vein seven day after surgery. Rats in sham group didn’t receive any injection. Control group considered as intact animals. The animals were evaluated for 14 days with modified limb placing and corner turn tests. The transplanted HUCB cells were also detected by immunohistochemistry. FINDINGS: Seven days after surgery, there was a significant recovery in the behavioral performance in the experimental group (12.7± 0.26) compared with sham group (10±0.4) and this significant difference continued by the day 14 (15.3±0.31 vs. 11.9±0.53, p<0.05). Postural and motor asymmetries, at the days 7 and 14 the experimental group showed a significant decrease in the percentage of right turns in comparison to the sham group (75%±3.07 and 59%±3.14 vs. 96%±2.21 and 97%±2.13, p<0.05). In addition, injured volume measurements disclosed a significant decrease in the experimental group compared with sham group (p<0.05). CONCLUSION: Intravenously transplanted HUCB stem cell can accelerate neurological function recovery and diminish the lesion size after the hypoxic ischemia. Thus these cells may provide a potential cell candidate for cell-based therapy in stroke.MR Nikravesh,H GhaffaripourM Jalali,D Hamidi Alamdari,J SanchooliM SeghatoleslamBabol University of Medical Sciencesarticlestem cellhypoxic ischemiabrain damageMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 4, Pp 22-29 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	stem cell hypoxic ischemia brain damage Medicine R Medicine (General) R5-920
spellingShingle	stem cell hypoxic ischemia brain damage Medicine R Medicine (General) R5-920 MR Nikravesh, H Ghaffaripour M Jalali, D Hamidi Alamdari, J Sanchooli M Seghatoleslam Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
description	BACKGROUND AND OBJECTIVE: Human umbilical cord blood (HUCB) is a rich source of stem cells. The aim of this study was the investigation of the therapeutic effects of these cells on cerebral ischemia in rat. METHODS: This study was carried out on 30 male Wistar rats (100-150 g). They were divided into three groups. Firstly, to create a laboratory model of ischemic stroke, right carotid artery of animals was occluded for 30 minutes in two groups of experimental and sham. Then, mononuclear cells of cord blood were isolated and labeled using bromodeoxyuridine (BrdU) and then 2×105 cells were injected into the experimental group via the tail vein seven day after surgery. Rats in sham group didn’t receive any injection. Control group considered as intact animals. The animals were evaluated for 14 days with modified limb placing and corner turn tests. The transplanted HUCB cells were also detected by immunohistochemistry. FINDINGS: Seven days after surgery, there was a significant recovery in the behavioral performance in the experimental group (12.7± 0.26) compared with sham group (10±0.4) and this significant difference continued by the day 14 (15.3±0.31 vs. 11.9±0.53, p<0.05). Postural and motor asymmetries, at the days 7 and 14 the experimental group showed a significant decrease in the percentage of right turns in comparison to the sham group (75%±3.07 and 59%±3.14 vs. 96%±2.21 and 97%±2.13, p<0.05). In addition, injured volume measurements disclosed a significant decrease in the experimental group compared with sham group (p<0.05). CONCLUSION: Intravenously transplanted HUCB stem cell can accelerate neurological function recovery and diminish the lesion size after the hypoxic ischemia. Thus these cells may provide a potential cell candidate for cell-based therapy in stroke.
format	article
author	MR Nikravesh, H Ghaffaripour M Jalali, D Hamidi Alamdari, J Sanchooli M Seghatoleslam
author_facet	MR Nikravesh, H Ghaffaripour M Jalali, D Hamidi Alamdari, J Sanchooli M Seghatoleslam
author_sort	MR Nikravesh,
title	Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
title_short	Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
title_full	Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
title_fullStr	Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
title_full_unstemmed	Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model
title_sort	therapeutic potential of cord blood stem cell in brain damage of an animal model
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/d750851cec0b454ba35b8eaad01b9d73
work_keys_str_mv	AT mrnikravesh therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel AT hghaffaripour therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel AT mjalali therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel AT dhamidialamdari therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel AT jsanchooli therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel AT mseghatoleslam therapeuticpotentialofcordbloodstemcellinbraindamageofananimalmodel
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Therapeutic Potential of Cord Blood Stem Cell in Brain Damage of an Animal Model

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