CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma

Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [bo...

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Autores principales:	Jun Lu, Gaojie Lou, Lin Jiang, Xiaoxing Liu, Jianxin Jiang, Xiaolin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glioblastoma circNUP98 miR-519a proliferation maturation Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/d751a5cce656474eb94a922f41b22bce
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spelling	oai:doaj.org-article:d751a5cce656474eb94a922f41b22bce2021-11-18T04:44:52ZCircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma1664-229510.3389/fneur.2021.679745https://doaj.org/article/d751a5cce656474eb94a922f41b22bce2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.679745/fullhttps://doaj.org/toc/1664-2295Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [both the mature and pre-mature microRNA (miRNA)] by quantitative real-time PCR (RT-qPCR) and heatmap analysis. The subcellular location that expresses circNUP98 was analyzed by nuclear fractionation assay. RNA pull-down assay was performed to evaluate the interaction between circNUP98 and pre-mature miR-519a-3p. Overexpression assays were performed to investigate the role of circNUP98 in the regulation of both the mature and pre-mature miR-519a-3p. The role of circNUP98 and miR-519a-3p in GB cell proliferation was explored by 5-bromo-2-deoxyuridine (BrdU) assay and was assessed in mouse xenograft model. Heatmap analysis showed that circNUP98 and pre-mature miR-519a-3p were upregulated in GB, while mature miR-519a-3p was downregulated in GB. Across the cancer tissues, circNUP98 was inversely correlated with mature miR-519a-3p, but positively correlated with pre-mature miR-519a-3p. In GB cells, circNUP98 was localized to both the nucleus and cytoplasm and it interacted with pre-mature miR-519a-3p. In GB cells, circNUP98 increased the expression levels of pre-mature miR-519a-3p and decreased the expression levels of mature miR-519a-3p. BrdU and cholecystokinin octapeptide (CCK-8) assays illustrated that overexpression of circNUP98 reduced the inhibitory effects of miR-519a-3p on cell proliferation. CircNUP98 contributed to larger tumors, which resulted in significantly reduced mice survival. CircNUP98 suppresses the maturation of miR-519a-3p to promote GB cell proliferation.Jun LuGaojie LouLin JiangXiaoxing LiuJianxin JiangXiaolin WangFrontiers Media S.A.articleglioblastomacircNUP98miR-519aproliferationmaturationNeurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma circNUP98 miR-519a proliferation maturation Neurology. Diseases of the nervous system RC346-429
spellingShingle	glioblastoma circNUP98 miR-519a proliferation maturation Neurology. Diseases of the nervous system RC346-429 Jun Lu Gaojie Lou Lin Jiang Xiaoxing Liu Jianxin Jiang Xiaolin Wang CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
description	Circular RNA (circNUP98) has been reported to promote renal cancer; however, its role in other cancers is unknown. The function of circNUP98 in glioblastoma (GB) cancer was explored in this study. A total of 58 GB tissue samples were collected to study the expression of circNUP98 and miR-519a-3p [both the mature and pre-mature microRNA (miRNA)] by quantitative real-time PCR (RT-qPCR) and heatmap analysis. The subcellular location that expresses circNUP98 was analyzed by nuclear fractionation assay. RNA pull-down assay was performed to evaluate the interaction between circNUP98 and pre-mature miR-519a-3p. Overexpression assays were performed to investigate the role of circNUP98 in the regulation of both the mature and pre-mature miR-519a-3p. The role of circNUP98 and miR-519a-3p in GB cell proliferation was explored by 5-bromo-2-deoxyuridine (BrdU) assay and was assessed in mouse xenograft model. Heatmap analysis showed that circNUP98 and pre-mature miR-519a-3p were upregulated in GB, while mature miR-519a-3p was downregulated in GB. Across the cancer tissues, circNUP98 was inversely correlated with mature miR-519a-3p, but positively correlated with pre-mature miR-519a-3p. In GB cells, circNUP98 was localized to both the nucleus and cytoplasm and it interacted with pre-mature miR-519a-3p. In GB cells, circNUP98 increased the expression levels of pre-mature miR-519a-3p and decreased the expression levels of mature miR-519a-3p. BrdU and cholecystokinin octapeptide (CCK-8) assays illustrated that overexpression of circNUP98 reduced the inhibitory effects of miR-519a-3p on cell proliferation. CircNUP98 contributed to larger tumors, which resulted in significantly reduced mice survival. CircNUP98 suppresses the maturation of miR-519a-3p to promote GB cell proliferation.
format	article
author	Jun Lu Gaojie Lou Lin Jiang Xiaoxing Liu Jianxin Jiang Xiaolin Wang
author_facet	Jun Lu Gaojie Lou Lin Jiang Xiaoxing Liu Jianxin Jiang Xiaolin Wang
author_sort	Jun Lu
title	CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_short	CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_full	CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_fullStr	CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_full_unstemmed	CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma
title_sort	circnup98 suppresses the maturation of mir-519a-3p in glioblastoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d751a5cce656474eb94a922f41b22bce
work_keys_str_mv	AT junlu circnup98suppressesthematurationofmir519a3pinglioblastoma AT gaojielou circnup98suppressesthematurationofmir519a3pinglioblastoma AT linjiang circnup98suppressesthematurationofmir519a3pinglioblastoma AT xiaoxingliu circnup98suppressesthematurationofmir519a3pinglioblastoma AT jianxinjiang circnup98suppressesthematurationofmir519a3pinglioblastoma AT xiaolinwang circnup98suppressesthematurationofmir519a3pinglioblastoma
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CircNUP98 Suppresses the Maturation of miR-519a-3p in Glioblastoma

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