Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications

Nikesh Gupta,1 Anju Shrivastava,2 Rakesh K Sharma11Nanotechnology and Drug Delivery Research Lab, Department of Chemistry, 2Department of Zoology, University of Delhi, Delhi, IndiaAbstract: Mesoporous silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase (HRP) have been sy...

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Autores principales:	Gupta N, Shrivastava A, Sharma RK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d7546ca648d64cb5ba2e53ced8b0f21b
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spelling	oai:doaj.org-article:d7546ca648d64cb5ba2e53ced8b0f21b2021-12-02T00:30:23ZSilica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications1176-91141178-2013https://doaj.org/article/d7546ca648d64cb5ba2e53ced8b0f21b2012-12-01T00:00:00Zhttp://www.dovepress.com/silica-nanoparticles-coencapsulating-gadolinium-oxide-and-horseradish--a11653https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Nikesh Gupta,1 Anju Shrivastava,2 Rakesh K Sharma11Nanotechnology and Drug Delivery Research Lab, Department of Chemistry, 2Department of Zoology, University of Delhi, Delhi, IndiaAbstract: Mesoporous silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase (HRP) have been synthesized in the aqueous core of sodium bis-(2-ethylhexyl)sulfosuccinate (AOT)&ndash;hexane&ndash;water reverse micelle. The average diameter of these silica particles is around 25 nm and the particles are spherical and highly monodispersed as depicted using transmission electron microscopy. The entrapment efficiency of HRP was found to be as high as 95%. Practically, the entrapped enzyme shows zero leachability up to 90 days. The enzyme entrapped in these silica nanoparticles follows Michaelis&ndash;Menten kinetics. Peroxidase entrapped in silica nanoparticles shows higher stability towards temperature and pH change as compared to free enzymes. The gadolinium oxide-doped silica nanoparticles are paramagnetic as observed from the nuclear magnetic resonance line-broadening effect on the proton spectrum of the surrounding water molecule. The entrapped enzyme, HRP, has been used to convert a benign prodrug, indole-3-acetic acid (IAA), to a toxic oxidized product and its toxic effect has been tested on cancerous cell lines through thiazolyl blue tetrazolium blue (MTT) assay. In vitro studies on different cancerous cell lines show that the enzyme has been entrapped and retains its activity inside the silica nanoparticles. IAA alone has no cytotoxic effect and it becomes active only after oxidative decarboxylation by HRP.Keywords: indole-3-acetic acid, HRP, free radical, cytotoxicity, MRIGupta NShrivastava ASharma RKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5491-5500 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Gupta N Shrivastava A Sharma RK Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
description	Nikesh Gupta,1 Anju Shrivastava,2 Rakesh K Sharma11Nanotechnology and Drug Delivery Research Lab, Department of Chemistry, 2Department of Zoology, University of Delhi, Delhi, IndiaAbstract: Mesoporous silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase (HRP) have been synthesized in the aqueous core of sodium bis-(2-ethylhexyl)sulfosuccinate (AOT)&ndash;hexane&ndash;water reverse micelle. The average diameter of these silica particles is around 25 nm and the particles are spherical and highly monodispersed as depicted using transmission electron microscopy. The entrapment efficiency of HRP was found to be as high as 95%. Practically, the entrapped enzyme shows zero leachability up to 90 days. The enzyme entrapped in these silica nanoparticles follows Michaelis&ndash;Menten kinetics. Peroxidase entrapped in silica nanoparticles shows higher stability towards temperature and pH change as compared to free enzymes. The gadolinium oxide-doped silica nanoparticles are paramagnetic as observed from the nuclear magnetic resonance line-broadening effect on the proton spectrum of the surrounding water molecule. The entrapped enzyme, HRP, has been used to convert a benign prodrug, indole-3-acetic acid (IAA), to a toxic oxidized product and its toxic effect has been tested on cancerous cell lines through thiazolyl blue tetrazolium blue (MTT) assay. In vitro studies on different cancerous cell lines show that the enzyme has been entrapped and retains its activity inside the silica nanoparticles. IAA alone has no cytotoxic effect and it becomes active only after oxidative decarboxylation by HRP.Keywords: indole-3-acetic acid, HRP, free radical, cytotoxicity, MRI
format	article
author	Gupta N Shrivastava A Sharma RK
author_facet	Gupta N Shrivastava A Sharma RK
author_sort	Gupta N
title	Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
title_short	Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
title_full	Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
title_fullStr	Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
title_full_unstemmed	Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
title_sort	silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/d7546ca648d64cb5ba2e53ced8b0f21b
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Silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications

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